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Characterization of the magnitude and mechanism of aldehyde oxidase-mediated nitric oxide production from nitrite

Li, H.; Kundu, T.K.; Zweier, J.L.; J. Biol. Chem. 284, 33850-33858 (2009)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.3.1
raloxifene
inhibits substrate binding at the molybdenum site of the enzyme
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.3.1
0.0096
-
4-(dimethylamino)cinnamaldehyde
in the presence of 1 mM nitrite, at pH 6.0 and 37C
Rattus norvegicus
1.2.3.1
0.024
-
NADH
in the presence of 1 mM nitrite, at pH 6.0 and 37C
Rattus norvegicus
1.2.3.1
2.7
-
nitrite
in the presence of 0.1 mM NADH, at pH 6.0 and 37C
Rattus norvegicus
1.2.3.1
3.3
-
nitrite
in the presence of 0.05 mM 4-(dimethylamino)cinnamaldehyde, at pH 6.0 and 37C
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.2.3.1
cytosol
-
Rattus norvegicus
5829
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.3.1
Rattus norvegicus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.2.3.1
liver
-
Rattus norvegicus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.3.1
4-(dimethylamino)cinnamaldehyde + H2O + O2
-
712399
Rattus norvegicus
?
-
-
-
?
1.2.3.1
4-(dimethylamino)cinnamaldehyde + nitrite + H2O + O2
in the presence of typical aldehyde substrates like 4-(dimethylamino)cinnamaldehyde or NADH, aldehyde oxidase reduces nitrite to nitric oxide
712399
Rattus norvegicus
? + nitric oxide
-
-
-
?
1.2.3.1
NADH + nitrite + H2O + O2
in the presence of typical aldehyde substrates like 4-(dimethylamino)cinnamaldehyde or NADH, aldehyde oxidase reduces nitrite to nitric oxide. NADH reacts with aldehyde oxidase at the FAD site of the enzyme
712399
Rattus norvegicus
? + nitric oxide
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.3.1
6
-
maximum aldehyde oxidase-catalyzed nitric oxide generation occurs at pH 6.0
Rattus norvegicus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.2.3.1
5
8
maximum aldehyde oxidase-catalyzed nitric oxide generation occurs at pH 6.0. When the pH is decreased to 5.0 or increased above 8.0, a decrease in the rate of nitric oxide generation is observed
Rattus norvegicus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.2.3.1
FAD
-
Rattus norvegicus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.2.3.1
FAD
-
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.3.1
raloxifene
inhibits substrate binding at the molybdenum site of the enzyme
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.3.1
0.0096
-
4-(dimethylamino)cinnamaldehyde
in the presence of 1 mM nitrite, at pH 6.0 and 37C
Rattus norvegicus
1.2.3.1
0.024
-
NADH
in the presence of 1 mM nitrite, at pH 6.0 and 37C
Rattus norvegicus
1.2.3.1
2.7
-
nitrite
in the presence of 0.1 mM NADH, at pH 6.0 and 37C
Rattus norvegicus
1.2.3.1
3.3
-
nitrite
in the presence of 0.05 mM 4-(dimethylamino)cinnamaldehyde, at pH 6.0 and 37C
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.2.3.1
cytosol
-
Rattus norvegicus
5829
-
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.2.3.1
liver
-
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.3.1
4-(dimethylamino)cinnamaldehyde + H2O + O2
-
712399
Rattus norvegicus
?
-
-
-
?
1.2.3.1
4-(dimethylamino)cinnamaldehyde + nitrite + H2O + O2
in the presence of typical aldehyde substrates like 4-(dimethylamino)cinnamaldehyde or NADH, aldehyde oxidase reduces nitrite to nitric oxide
712399
Rattus norvegicus
? + nitric oxide
-
-
-
?
1.2.3.1
NADH + nitrite + H2O + O2
in the presence of typical aldehyde substrates like 4-(dimethylamino)cinnamaldehyde or NADH, aldehyde oxidase reduces nitrite to nitric oxide. NADH reacts with aldehyde oxidase at the FAD site of the enzyme
712399
Rattus norvegicus
? + nitric oxide
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.3.1
6
-
maximum aldehyde oxidase-catalyzed nitric oxide generation occurs at pH 6.0
Rattus norvegicus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.2.3.1
5
8
maximum aldehyde oxidase-catalyzed nitric oxide generation occurs at pH 6.0. When the pH is decreased to 5.0 or increased above 8.0, a decrease in the rate of nitric oxide generation is observed
Rattus norvegicus