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Literature summary extracted from

  • Vomund, A.N.; Majerus, E.M.
    ADAMTS13 bound to endothelial cells exhibits enhanced cleavage of von Willebrand factor (2009), J. Biol. Chem., 284, 30925-30932.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.87 expression of His-tagged ADAMTS13 Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.87 heparin inhibits ADAMTS13 activity through inhibition of enzyme binding to endothelial cell surfaces, overview Homo sapiens
3.4.24.87 Trypsin inhibits ADAMTS13 activity through inhibition of enzyme binding to endothelial cell surfaces, overview Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.87 cell surface ADAMTS13 binds to endothelial cells in a specific, reversible, and time-dependent manner with a Kd of 58 nM. Binding requires the COOH-terminal thrombospondin type 1 repeats of the protease. Binding is inhibited in the presence of heparin and by trypsin treatment of the cells Homo sapiens 9986
-
3.4.24.87 extracellular
-
 Homo sapiens
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.87 von Willebrand factor + H2O Homo sapiens cleavage can also occur at the surface of endothelial cells. ADAMTS13 that is prebound to endothelial cells exhibits increased proteolysis of VWF as compared with ADAMTS13 present only in solution. Thus, cleavage of VWF occurs mainly at the endothelial cell surface von Willebrand factor fragments
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.87 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.87 recombinant His-tagged ADAMTS13 by nickel affinity chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.87 endothelial cell at the surface of endothelial cells Homo sapiens
-
3.4.24.87 plasma
-
 Homo sapiens
-
3.4.24.87 umbilical vein endothelial cell at the surface of endothelial cells Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.87 von Willebrand factor + H2O
-
 Homo sapiens von Willebrand factor fragments
-
 ?
3.4.24.87 von Willebrand factor + H2O cleavage can also occur at the surface of endothelial cells. ADAMTS13 that is prebound to endothelial cells exhibits increased proteolysis of VWF as compared with ADAMTS13 present only in solution. Thus, cleavage of VWF occurs mainly at the endothelial cell surface Homo sapiens von Willebrand factor fragments
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.24.87 ADAMTS13
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.87 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.87 7.5
-
 assay at Homo sapiens

General Information

EC Number General Information Comment Organism
3.4.24.87 physiological function ADAMTS13 is a plasma metalloprotease that cleaves ultra-large von Willebrand factor multimers to generate less thrombogenic fragments Homo sapiens