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Literature summary extracted from

  • De Pascale, G.; Lloyd, A.J.; Schouten, J.A.; Gilbey, a.M.; Roper, D.I.; Dowson, C.G.; Bugg, T.D.H.
    Kinetic characterization of lipid II-Ala:alanyl-tRNA ligase (MurN) from Streptococcus pneumoniae using semisynthetic aminoacyl-lipid II substrates (2008), J. Biol. Chem., 283, 34751-34759.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.B2 expression in Escherichia coli Streptococcus pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.2.B2 33.1
-
 UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine pH 6.8, 37°C Streptococcus pneumoniae
2.3.2.B2 40
-
 UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine pH 6.8, 37°C Streptococcus pneumoniae
2.3.2.B2 125
-
 UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryl)-D-alanyl-D-alanine pH 6.8, 37°C Streptococcus pneumoniae
2.3.2.B2 146
-
 UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryl)-D-alanyl-D-alanine pH 6.8, 37°C Streptococcus pneumoniae

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.B2 Streptococcus pneumoniae
-
 penicillin-resistant strain
-
2.3.2.B2 Streptococcus pneumoniae
-
 penicillin-sensitive strain
-
2.3.2.B2 Streptococcus pneumoniae 159
-
 penicillin-resistant strain
-
2.3.2.B2 Streptococcus pneumoniae Pn16
-
 penicillin-sensitive strain
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.2.B2 0.7
-
 substrate UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryl)-D-alanyl-D-alanine, pH 6.8, 37°C Streptococcus pneumoniae
2.3.2.B2 0.9
-
 substrate UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryl)-D-alanyl-D-alanine, pH 6.8, 37°C Streptococcus pneumoniae
2.3.2.B2 8.7
-
 substrate UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine, pH 6.8, 37°C Streptococcus pneumoniae
2.3.2.B2 11
-
 substrate UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine, pH 6.8, 37°C Streptococcus pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.B2 additional information no substrate: UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine, i.e. lipid I. No addition of L-serine to alanyl-lipid I Streptococcus pneumoniae ?
-
 ?
2.3.2.B2 additional information no substrate: UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine, i.e. lipid I. No addition of L-serine to alanyl-lipid I Streptococcus pneumoniae 159 ?
-
 ?
2.3.2.B2 additional information no substrate: UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine, i.e. lipid I. No addition of L-serine to alanyl-lipid I Streptococcus pneumoniae Pn16 ?
-
 ?
2.3.2.B2 UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine + L-alanyl-tRNA
-
 Streptococcus pneumoniae UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl-L-alanyl)-D-alanyl-D-alanine + tRNA
-
 ?
2.3.2.B2 UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine + L-alanyl-tRNA i.e. N6-alanyl-lipid I Streptococcus pneumoniae UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl-L-alanyl)-D-alanyl-D-alanine + tRNA
-
 ?
2.3.2.B2 UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine + L-alanyl-tRNA
-
 Streptococcus pneumoniae 159 UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl-L-alanyl)-D-alanyl-D-alanine + tRNA
-
 ?
2.3.2.B2 UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine + L-alanyl-tRNA i.e. N6-alanyl-lipid I Streptococcus pneumoniae 159 UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl-L-alanyl)-D-alanyl-D-alanine + tRNA
-
 ?
2.3.2.B2 UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine + L-alanyl-tRNA
-
 Streptococcus pneumoniae Pn16 UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl-L-alanyl)-D-alanyl-D-alanine + tRNA
-
 ?
2.3.2.B2 UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine + L-alanyl-tRNA i.e. N6-alanyl-lipid I Streptococcus pneumoniae Pn16 UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl-L-alanyl)-D-alanyl-D-alanine + tRNA
-
 ?
2.3.2.B2 UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryl)-D-alanyl-D-alanine + L-alanyl-tRNA
-
 Streptococcus pneumoniae UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryll-L-alanyl)-D-alanyl-D-alanine + tRNA
-
 ?
2.3.2.B2 UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryl)-D-alanyl-D-alanine + L-alanyl-tRNA i.e. N6-seryl-lipid I Streptococcus pneumoniae UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryll-L-alanyl)-D-alanyl-D-alanine + tRNA
-
 ?
2.3.2.B2 UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryl)-D-alanyl-D-alanine + L-alanyl-tRNA
-
 Streptococcus pneumoniae 159 UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryll-L-alanyl)-D-alanyl-D-alanine + tRNA
-
 ?
2.3.2.B2 UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryl)-D-alanyl-D-alanine + L-alanyl-tRNA i.e. N6-seryl-lipid I Streptococcus pneumoniae 159 UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryll-L-alanyl)-D-alanyl-D-alanine + tRNA
-
 ?
2.3.2.B2 UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryl)-D-alanyl-D-alanine + L-alanyl-tRNA
-
 Streptococcus pneumoniae Pn16 UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryll-L-alanyl)-D-alanyl-D-alanine + tRNA
-
 ?
2.3.2.B2 UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryl)-D-alanyl-D-alanine + L-alanyl-tRNA i.e. N6-seryl-lipid I Streptococcus pneumoniae Pn16 UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryll-L-alanyl)-D-alanyl-D-alanine + tRNA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.2.B2 lipid II-Ala:alanyl-tRNA ligase
-
 Streptococcus pneumoniae
2.3.2.B2 MurN
-
 Streptococcus pneumoniae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.2.B2 0.02
-
 UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryl)-D-alanyl-D-alanine pH 6.8, 37°C Streptococcus pneumoniae
2.3.2.B2 0.06
-
 UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine pH 6.8, 37°C Streptococcus pneumoniae
2.3.2.B2 0.07
-
 UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine pH 6.8, 37°C Streptococcus pneumoniae

General Information

EC Number General Information Comment Organism
2.3.2.B2 physiological function enzyme is involved in formation of branched peptidoglycan peptide cross-bridges L-Ala-L-Ala or L-Ser-L-Ala in Streptococcus pneumoniae Streptococcus pneumoniae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.2.B2 0.112
-
 UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryl)-D-alanyl-D-alanine pH 6.8, 37°C Streptococcus pneumoniae
2.3.2.B2 0.138
-
 UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryl)-D-alanyl-D-alanine pH 6.8, 37°C Streptococcus pneumoniae
2.3.2.B2 1.579
-
 UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine pH 6.8, 37°C Streptococcus pneumoniae
2.3.2.B2 2.186
-
 UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine pH 6.8, 37°C Streptococcus pneumoniae