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Literature summary extracted from

  • Shao, Z.; Blodgett, J.A.V.; Circello, B.T.; Eliot, A.C.; Woodyer, R.; Li, G.; van der Donk, W.A.; Metcalf, W.W.; Zhao, H.
    Biosynthesis of 2-hydroxyethylphosphonate, an unexpected intermediate common to multiple phosphonate biosynthetic pathways (2008), J. Biol. Chem., 283, 23161-23168.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.99.B6 expressed in Escherichia coli BL21(DE3) cells as His6-tagged enzyme Streptomyces wedmorensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.309 EDTA approximately 0.02-0.025 mM PhpC is incubated with 20-25 mM EDTA at 4°C until the activity is completely abolished (usually 1-2 h) Streptomyces viridochromogenes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.309 0.0193
-
 NADH in 50 mM HEPES, 200 mM NaCl, pH 7.5, temperature not specified in the publication Streptomyces viridochromogenes
1.1.1.309 0.185
-
 phosphonoacetaldehyde in 50 mM HEPES, 200 mM NaCl, pH 7.5, temperature not specified in the publication Streptomyces viridochromogenes
1.1.99.B6 0.0098
-
 NADPH in 50 mM HEPES, 200 mM NaCl, pH 7.5, temperature not specified in the publication Streptomyces wedmorensis
1.1.99.B6 0.031
-
 phosphonoacetaldehyde in 50 mM HEPES, 200 mM NaCl, pH 7.5, temperature not specified in the publication Streptomyces wedmorensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.309 Fe2+ the iron content of PhpC is less than 5% Streptomyces viridochromogenes
1.1.1.309 Zn2+ Zn2+ is required for enzymatic activity Streptomyces viridochromogenes

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.309 Streptomyces viridochromogenes
-
-
-
1.1.99.B6 Streptomyces wedmorensis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.309
-
 Streptomyces viridochromogenes
1.1.99.B6 Talon immobilized metal affinity chromatography and Superdex 200 gel filtration Streptomyces wedmorensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.309 2-hydroxyethylphosphonate + NAD+ the catalytic efficiency of phosphonoacetaldehyde reduction is 377fold greater than that of the reverse reaction, and even at pH 9.0, the catalytic efficiency of the forward reaction is still 11fold greater than that of the reverse reaction Streptomyces viridochromogenes phosphonoacetaldehyde + NADH + H+
-
 r
1.1.1.309 additional information no reaction is observed with hydroxymethylphosphonate and 3-hydroxypropylphosphonate Streptomyces viridochromogenes ?
-
 ?
1.1.99.B6 2-hydroxyethylphosphonate + NADP+
-
 Streptomyces wedmorensis phosphonoacetaldehyde + NADPH + H+
-
 r
1.1.99.B6 additional information no reaction is observed with hydroxymethylphosphonate and 3-hydroxypropylphosphonate Streptomyces wedmorensis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.309 monomer gel filtration Streptomyces viridochromogenes

Synonyms

EC Number Synonyms Comment Organism
1.1.1.309 PhpC
-
 Streptomyces viridochromogenes
1.1.99.B6 FomC
-
 Streptomyces wedmorensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.309 0.41
-
 phosphonoacetaldehyde in 50 mM HEPES, 200 mM NaCl, pH 7.5, temperature not specified in the publication Streptomyces viridochromogenes
1.1.99.B6 1.3
-
 phosphonoacetaldehyde in 50 mM HEPES, 200 mM NaCl, pH 7.5, temperature not specified in the publication Streptomyces wedmorensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.309 7
-
 phosphonoacetaldehyde reduction is more favorable under neutral conditions (optimal pH at 7.0) Streptomyces viridochromogenes
1.1.1.309 9
-
 2-hydroxyethylphosphonate oxidation is favored under basic conditions (optimal pH at 9.0) Streptomyces viridochromogenes
1.1.99.B6 7
-
 phosphonoacetaldehyde reduction is more favorable under neutral conditions (optimal pH at 7.0) Streptomyces wedmorensis
1.1.99.B6 9
-
 2-hydroxyethylphosphonate oxidation is favored under basic conditions (optimal pH at 9.0) Streptomyces wedmorensis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.309 NADH PhpC is an NADH-dependent enzyme Streptomyces viridochromogenes
1.1.99.B6 NADPH FomC is an NADPH-dependent enzyme Streptomyces wedmorensis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.309 2.2
-
 phosphonoacetaldehyde in 50 mM HEPES, 200 mM NaCl, pH 7.5, temperature not specified in the publication Streptomyces viridochromogenes
1.1.99.B6 41
-
 phosphonoacetaldehyde in 50 mM HEPES, 200 mM NaCl, pH 7.5, temperature not specified in the publication Streptomyces wedmorensis