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Literature summary extracted from

  • Hanano, A.; Burcklen, M.; Flenet, M.; Ivancich, A.; Louwagie, M.; Garin, J.; Blee, E.
    Plant seed peroxygenase is an original heme-oxygenase with an EF-hand calcium binding motif (2006), J. Biol. Chem., 281, 33140-33151.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.2.3 expressed in Saccharomyces cerevisiae Arabidopsis thaliana
1.11.2.3 expressed in Saccharomyces cerevisiae Avena sativa

Protein Variants

EC Number Protein Variants Comment Organism
1.11.2.3 H138V the mutant possess only 4% of the heme content wild type isoform PXG1 Arabidopsis thaliana
1.11.2.3 H70V the heme content of the mutant is barely measurable (less than 1%) as compared to the wild type isoform PXG1 Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.2.3 2-mercaptoethanol the sulfoxidation of thiobenzamide, catalyzed by isoform PXG1, is completely abolished in the presence of 1 mM 2-mercaptoethanol (competitive inhibitor) Arabidopsis thaliana
1.11.2.3 terbufos a suicide substrate for plant peroxygenases, and at a concentration of 3 mM, it effectively inactivates the activity of isoform PXG1 Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.11.2.3 membrane integral membrane protein Avena sativa 16020
-
1.11.2.3 membrane integral membrane protein Arabidopsis thaliana 16020
-
1.11.2.3 microsome
-
 Avena sativa
-
-
1.11.2.3 microsome
-
 Arabidopsis thaliana
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.11.2.3 Ca2+ the enzyme contains a Ca2+ binding motif, calcium is crucial for its peroxygenase activity Arabidopsis thaliana
1.11.2.3 Ca2+ the enzyme contains a Ca2+ binding motif, calcium is crucial for peroxygenase activity Avena sativa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.2.3 27000
-
 SDS-PAGE Avena sativa
1.11.2.3 32000
-
 SDS-PAGE Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.11.2.3 aniline + cumene hydroperoxide Avena sativa
-
 ?
-
 ?
1.11.2.3 aniline + cumene hydroperoxide Arabidopsis thaliana
-
 ?
-
 ?
1.11.2.3 linoleic acid + cumene hydroperoxide Avena sativa
-
 ?
-
 ?
1.11.2.3 linoleic acid + cumene hydroperoxide Arabidopsis thaliana
-
 ?
-
 ?
1.11.2.3 methyl 4-tolyl sulfide + cumene hydroperoxide Avena sativa
-
 ?
-
 ?
1.11.2.3 methyl 4-tolyl sulfide + cumene hydroperoxide Arabidopsis thaliana
-
 ?
-
 ?
1.11.2.3 oleic acid + cumene hydroperoxide Avena sativa
-
 cis-9,10-epoxystearic acid + ?
-
 ?
1.11.2.3 oleic acid + cumene hydroperoxide Arabidopsis thaliana
-
 cis-9,10-epoxystearic acid + ?
-
 ?
1.11.2.3 thiobenzamide + cumene hydroperoxide Avena sativa
-
 ?
-
 ?
1.11.2.3 thiobenzamide + cumene hydroperoxide Arabidopsis thaliana
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.11.2.3 Arabidopsis thaliana
-
-
-
1.11.2.3 Avena sativa
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.11.2.3 glycoprotein
-
 Avena sativa
1.11.2.3 glycoprotein
-
 Arabidopsis thaliana
1.11.2.3 sumoylation isoform PXG1 possesses a high sumoylation potentiality at the position Lys-196 Arabidopsis thaliana

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.2.3 DEAE-Trisacryl 300 gel filtration and CM-Sepharose column chromatography Arabidopsis thaliana
1.11.2.3 Ni-NTA column chromatography Avena sativa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.11.2.3 seed
-
 Avena sativa
-
1.11.2.3 seed
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.2.3 aniline + cumene hydroperoxide
-
 Avena sativa ?
-
 ?
1.11.2.3 aniline + cumene hydroperoxide
-
 Arabidopsis thaliana ?
-
 ?
1.11.2.3 linoleic acid + cumene hydroperoxide
-
 Avena sativa ?
-
 ?
1.11.2.3 linoleic acid + cumene hydroperoxide
-
 Arabidopsis thaliana ?
-
 ?
1.11.2.3 methyl 4-tolyl sulfide + cumene hydroperoxide
-
 Avena sativa ?
-
 ?
1.11.2.3 methyl 4-tolyl sulfide + cumene hydroperoxide
-
 Arabidopsis thaliana ?
-
 ?
1.11.2.3 oleic acid + cumene hydroperoxide
-
 Avena sativa cis-9,10-epoxystearic acid + ?
-
 ?
1.11.2.3 oleic acid + cumene hydroperoxide
-
 Arabidopsis thaliana cis-9,10-epoxystearic acid + ?
-
 ?
1.11.2.3 thiobenzamide + cumene hydroperoxide
-
 Avena sativa ?
-
 ?
1.11.2.3 thiobenzamide + cumene hydroperoxide
-
 Arabidopsis thaliana ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.11.2.3 caleosin
-
 Avena sativa
1.11.2.3 caleosin
-
 Arabidopsis thaliana
1.11.2.3 plant peroxygenase
-
 Avena sativa
1.11.2.3 plant peroxygenase
-
 Arabidopsis thaliana
1.11.2.3 PXG
-
 Avena sativa
1.11.2.3 PXG1 isoform Arabidopsis thaliana
1.11.2.3 PXG2 isoform Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.2.3 heme
-
 Avena sativa
1.11.2.3 heme isoform PXG1 is a hemoprotein Arabidopsis thaliana