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Literature summary extracted from

  • Frohlich, K.M.; Roberts, R.A.; Housley, N.A.; Audia, J.P.
    Rickettsia prowazekii uses an sn-glycerol-3-phosphate dehydrogenase and a novel dihydroxyacetone phosphate transport system to supply triose phosphate for phospholipid biosynthesis (2010), J. Bacteriol., 192, 4281-4288.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.94 expression in Escherichia coli Rickettsia prowazekii

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.94 additional information heterologous expression in Escherichia coli complements an Escherichia coli gpsA mutant Rickettsia prowazekii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.94 0.048
-
 NADPH pH 7.4, temperature not specified in the publication Rickettsia prowazekii
1.1.1.94 0.528
-
 glycerone phosphate cosubstrate NADPH, pH 7.4, temperature not specified in the publication Rickettsia prowazekii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.94 36600
-
 x * 36600, SDS-PAGE and calculated for His-tagged protein Rickettsia prowazekii

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.94 Rickettsia prowazekii Q9ZDA0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.94 recombinant protein Rickettsia prowazekii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.94 glycerone phosphate + NADH + H+
-
 Rickettsia prowazekii sn-glycerol 3-phosphate + NAD+
-
 r
1.1.1.94 glycerone phosphate + NADPH + H+ favoured reaction direction Rickettsia prowazekii sn-glycerol 3-phosphate + NADP+
-
 r
1.1.1.94 sn-glycerol 3-phosphate + NADP+
-
 Rickettsia prowazekii glycerone phosphate + NADPH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.94 ? x * 36600, SDS-PAGE and calculated for His-tagged protein Rickettsia prowazekii

Synonyms

EC Number Synonyms Comment Organism
1.1.1.94 G3PDH
-
 Rickettsia prowazekii
1.1.1.94 GpsA
-
 Rickettsia prowazekii
1.1.1.94 RP442
-
 Rickettsia prowazekii

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.94 NADH NADPH is preferred over NADH as the cofactor Rickettsia prowazekii
1.1.1.94 NADPH NADPH is preferred over NADH as the cofactor Rickettsia prowazekii

General Information

EC Number General Information Comment Organism
1.1.1.94 physiological function Rickettsia prowazekii is unable to synthesize glycerone phosphate as a substrate for the GpsA enzymatic reaction. Purified Rickettsia prowazekii transports and incorporates glycerone phosphate into phospholipids, implicating a role for GpsA in vivo as part of a rickettsial G3P acquisition pathway for phospholipid biosynthesis Rickettsia prowazekii