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Literature summary extracted from

  • Seffernick, J.L.; Dodge, A.G.; Sadowsky, M.J.; Bumpus, J.A.; Wackett, L.P.
    Bacterial ammeline metabolism via guanine deaminase (2010), J. Bacteriol., 192, 1106-1112.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.4.3 expressed in Escherichia BL21(DE3) pLysS cells Bradyrhizobium japonicum

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.3 Bradyrhizobium japonicum
-
-
-
3.5.4.3 Bradyrhizobium japonicum USDA 110
-
-
-
3.5.4.3 Escherichia coli
-
-
-
3.5.4.3 Homo sapiens Q9Y2T3
-
-
3.5.4.3 Pseudomonas putida
-
-
-
3.5.4.3 Pseudomonas putida KT 2240
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.3 HiTrap IMAC column chromatography Bradyrhizobium japonicum
3.5.4.3 HiTrap IMAC column chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.3 ammeline + H2O
-
 Escherichia coli ammelide + NH3
-
 ir
3.5.4.3 ammeline + H2O
-
 Bradyrhizobium japonicum ammelide + NH3
-
 ir
3.5.4.3 ammeline + H2O
-
 Pseudomonas putida ammelide + NH3
-
 ir
3.5.4.3 ammeline + H2O
-
 Homo sapiens ammelide + NH3
-
 ir
3.5.4.3 ammeline + H2O
-
 Bradyrhizobium japonicum USDA 110 ammelide + NH3
-
 ir
3.5.4.3 ammeline + H2O
-
 Pseudomonas putida KT 2240 ammelide + NH3
-
 ir
3.5.4.3 guanine + H2O
-
 Escherichia coli xanthine + NH3
-
 ?
3.5.4.3 guanine + H2O
-
 Bradyrhizobium japonicum xanthine + NH3
-
 ?
3.5.4.3 guanine + H2O
-
 Pseudomonas putida xanthine + NH3
-
 ?
3.5.4.3 guanine + H2O
-
 Homo sapiens xanthine + NH3
-
 ?
3.5.4.3 guanine + H2O
-
 Bradyrhizobium japonicum USDA 110 xanthine + NH3
-
 ?
3.5.4.3 guanine + H2O
-
 Pseudomonas putida KT 2240 xanthine + NH3
-
 ?
3.5.4.3 additional information the enzyme does not metabolize ammelide or melamine Escherichia coli ?
-
 ?
3.5.4.3 additional information the enzyme does not metabolize ammelide or melamine Bradyrhizobium japonicum ?
-
 ?
3.5.4.3 additional information the enzyme does not metabolize ammelide or melamine Pseudomonas putida ?
-
 ?
3.5.4.3 additional information the enzyme does not metabolize ammelide or melamine Homo sapiens ?
-
 ?
3.5.4.3 additional information the enzyme does not metabolize ammelide or melamine Bradyrhizobium japonicum USDA 110 ?
-
 ?
3.5.4.3 additional information the enzyme does not metabolize ammelide or melamine Pseudomonas putida KT 2240 ?
-
 ?

General Information

EC Number General Information Comment Organism
3.5.4.3 malfunction an guanine deaminase deletion mutant is deficient in ammeline deaminase activity Escherichia coli
3.5.4.3 physiological function guanine deaminase catalyzes ammeline deamination at rates sufficient to support bacterial growth on ammeline as a sole nitrogen source Escherichia coli
3.5.4.3 physiological function guanine deaminase catalyzes ammeline deamination at rates sufficient to support bacterial growth on ammeline as a sole nitrogen source Bradyrhizobium japonicum
3.5.4.3 physiological function guanine deaminase catalyzes ammeline deamination at rates sufficient to support bacterial growth on ammeline as a sole nitrogen source Pseudomonas putida