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The iron-hydrogenase of Thermotoga maritima utilizes ferredoxin and NADH synergistically: a new perspective on anaerobic hydrogen production

Schut, G.J.; Adams, M.W.; J. Bacteriol. 191, 4451-4457 (2009)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.12.1.2
0.00025
-
reduced ferredoxin
pH and temperature not specified in the publication
Thermotoga maritima
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.12.1.2
cytoplasm
-
Thermotoga maritima
5737
-
1.12.1.4
cytoplasm
-
Thermotoga maritima
5737
-
1.12.7.2
cytoplasm
-
Thermotoga maritima
5737
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.12.1.4
NADH + H+ + reduced ferredoxin
Thermotoga maritima
the iron hydrogenase requires the presence of both electron carriers, NADH and ferredoxin, for catalysis of H2 production
H2 + NAD+ + oxidized ferredoxin
-
-
?
1.12.1.4
NADH + H+ + reduced ferredoxin
Thermotoga maritima DSM 3109
the iron hydrogenase requires the presence of both electron carriers, NADH and ferredoxin, for catalysis of H2 production
H2 + NAD+ + oxidized ferredoxin
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.12.1.2
Thermotoga maritima
-
-
-
1.12.1.4
Thermotoga maritima
-
-
-
1.12.1.4
Thermotoga maritima DSM 3109
-
-
-
1.12.7.2
Thermotoga maritima
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.12.1.2
H+ + NADH + reduced ferredoxin
the hydrogenase requires the presence of both electron carriers, NADH and reduced ferredoxin, synergistically in a 1:1 ratio for catalysis of H2 production
712281
Thermotoga maritima
H2 + NAD+ + oxidized ferredoxin
-
-
-
?
1.12.1.2
H+ + NADH + reduced ferredoxin
the hydrogenase requires the presence of both electron carriers, NADH and reduced ferredoxin, synergistically in a 1:1 ratio for catalysis of H2 production
712281
Thermotoga maritima DSM 3109
H2 + NAD+ + oxidized ferredoxin
-
-
-
?
1.12.1.2
H+ + reduced dithionite
-
712281
Thermotoga maritima
H2 + oxidized dithionite
-
-
-
?
1.12.1.2
H+ + reduced dithionite
-
712281
Thermotoga maritima DSM 3109
H2 + oxidized dithionite
-
-
-
?
1.12.1.2
H+ + reduced methyl viologen
-
712281
Thermotoga maritima
H2 + oxidized methyl viologen
-
-
-
?
1.12.1.2
H+ + reduced methyl viologen
-
712281
Thermotoga maritima DSM 3109
H2 + oxidized methyl viologen
-
-
-
?
1.12.1.4
NADH + H+ + reduced ferredoxin
the iron hydrogenase requires the presence of both electron carriers, NADH and ferredoxin, for catalysis of H2 production
712281
Thermotoga maritima
H2 + NAD+ + oxidized ferredoxin
-
-
-
?
1.12.1.4
NADH + H+ + reduced ferredoxin
the iron hydrogenase requires the presence of both electron carriers, NADH and ferredoxin, for catalysis of H2 production
712281
Thermotoga maritima DSM 3109
H2 + NAD+ + oxidized ferredoxin
-
-
-
?
1.12.1.4
NADH + H+ + reduced methyl viologen
-
712281
Thermotoga maritima
H2 + NAD+ + oxidized methyl viologen
-
-
-
?
1.12.7.2
H2 + oxidized ferredoxin
-
712281
Thermotoga maritima
H+ + reduced ferredoxin
-
-
-
?
1.12.7.2
H2 + oxidized ferredoxin
-
712281
Thermotoga maritima DSM 3109
H+ + reduced ferredoxin
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.12.1.4
heterotrimer
-
Thermotoga maritima
1.12.7.2
monomer
-
Thermotoga maritima
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.12.1.2
Ferredoxin
the hydrogenase requires the presence of both electron carriers, NADH and reduced ferredoxin, synergistically for catalysis of H2 production
Thermotoga maritima
1.12.1.2
FMN
-
Thermotoga maritima
1.12.1.2
NADH
the hydrogenase requires the presence of both electron carriers, NADH and reduced ferredoxin, synergistically for catalysis of H2 production
Thermotoga maritima
1.12.1.4
FMN
-
Thermotoga maritima
1.12.1.4
iron-sulfur centre
the alpha subunit contains two [2Fe-2S] clusters and three [4Fe-4S] clusters, the beta subunit contains three [4Fe-4S] clusters and one [2Fe-2S] clusters, the gamma subunit contains one [2Fe-2S] cluster
Thermotoga maritima
1.12.7.2
Ferredoxin
-
Thermotoga maritima
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.12.1.2
Ferredoxin
the hydrogenase requires the presence of both electron carriers, NADH and reduced ferredoxin, synergistically for catalysis of H2 production
Thermotoga maritima
1.12.1.2
FMN
-
Thermotoga maritima
1.12.1.2
NADH
the hydrogenase requires the presence of both electron carriers, NADH and reduced ferredoxin, synergistically for catalysis of H2 production
Thermotoga maritima
1.12.1.4
FMN
-
Thermotoga maritima
1.12.1.4
iron-sulfur centre
the alpha subunit contains two [2Fe-2S] clusters and three [4Fe-4S] clusters, the beta subunit contains three [4Fe-4S] clusters and one [2Fe-2S] clusters, the gamma subunit contains one [2Fe-2S] cluster
Thermotoga maritima
1.12.7.2
Ferredoxin
-
Thermotoga maritima
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.12.1.2
0.00025
-
reduced ferredoxin
pH and temperature not specified in the publication
Thermotoga maritima
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.12.1.2
cytoplasm
-
Thermotoga maritima
5737
-
1.12.1.4
cytoplasm
-
Thermotoga maritima
5737
-
1.12.7.2
cytoplasm
-
Thermotoga maritima
5737
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.12.1.4
NADH + H+ + reduced ferredoxin
Thermotoga maritima
the iron hydrogenase requires the presence of both electron carriers, NADH and ferredoxin, for catalysis of H2 production
H2 + NAD+ + oxidized ferredoxin
-
-
?
1.12.1.4
NADH + H+ + reduced ferredoxin
Thermotoga maritima DSM 3109
the iron hydrogenase requires the presence of both electron carriers, NADH and ferredoxin, for catalysis of H2 production
H2 + NAD+ + oxidized ferredoxin
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.12.1.2
H+ + NADH + reduced ferredoxin
the hydrogenase requires the presence of both electron carriers, NADH and reduced ferredoxin, synergistically in a 1:1 ratio for catalysis of H2 production
712281
Thermotoga maritima
H2 + NAD+ + oxidized ferredoxin
-
-
-
?
1.12.1.2
H+ + NADH + reduced ferredoxin
the hydrogenase requires the presence of both electron carriers, NADH and reduced ferredoxin, synergistically in a 1:1 ratio for catalysis of H2 production
712281
Thermotoga maritima DSM 3109
H2 + NAD+ + oxidized ferredoxin
-
-
-
?
1.12.1.2
H+ + reduced dithionite
-
712281
Thermotoga maritima
H2 + oxidized dithionite
-
-
-
?
1.12.1.2
H+ + reduced dithionite
-
712281
Thermotoga maritima DSM 3109
H2 + oxidized dithionite
-
-
-
?
1.12.1.2
H+ + reduced methyl viologen
-
712281
Thermotoga maritima
H2 + oxidized methyl viologen
-
-
-
?
1.12.1.2
H+ + reduced methyl viologen
-
712281
Thermotoga maritima DSM 3109
H2 + oxidized methyl viologen
-
-
-
?
1.12.1.4
NADH + H+ + reduced ferredoxin
the iron hydrogenase requires the presence of both electron carriers, NADH and ferredoxin, for catalysis of H2 production
712281
Thermotoga maritima
H2 + NAD+ + oxidized ferredoxin
-
-
-
?
1.12.1.4
NADH + H+ + reduced ferredoxin
the iron hydrogenase requires the presence of both electron carriers, NADH and ferredoxin, for catalysis of H2 production
712281
Thermotoga maritima DSM 3109
H2 + NAD+ + oxidized ferredoxin
-
-
-
?
1.12.1.4
NADH + H+ + reduced methyl viologen
-
712281
Thermotoga maritima
H2 + NAD+ + oxidized methyl viologen
-
-
-
?
1.12.7.2
H2 + oxidized ferredoxin
-
712281
Thermotoga maritima
H+ + reduced ferredoxin
-
-
-
?
1.12.7.2
H2 + oxidized ferredoxin
-
712281
Thermotoga maritima DSM 3109
H+ + reduced ferredoxin
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.12.1.4
heterotrimer
-
Thermotoga maritima
1.12.7.2
monomer
-
Thermotoga maritima