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Literature summary extracted from

  • Wischgoll, S.; Taubert, M.; Peters, F.; Jehmlich, N.; von Bergen, M.; Boll, M.
    Decarboxylating and nondecarboxylating glutaryl-coenzyme A dehydrogenases in the aromatic metabolism of obligately anaerobic bacteria (2009), J. Bacteriol., 191, 4401-4409.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.99.32 screening of a cosmid gene library, DNA and amino acid sequence determination and analysis, expression of His6-tagged GDHDes in Escherichia coli strain BL21(DE3) Desulfococcus multivorans

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.8.6 additional information no inhibition by glutaconyl-CoA Geobacter metallireducens
1.3.99.32 glutaconyl-CoA product inhibition Desulfococcus multivorans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.8.6 0.03
-
 glutaryl-CoA pH 7.8, 30°C Geobacter metallireducens
1.3.99.32 0.052
-
 glutaryl-CoA pH 7.8, 30°C Desulfococcus multivorans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.8.6 mitochondrial inner membrane
-
 Geobacter metallireducens 5743
-
1.3.99.32 mitochondrial inner membrane
-
 Desulfococcus multivorans 5743
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.8.6 glutaryl-CoA + FAD Geobacter metallireducens via intermediate glutaconyl-CoA crotonoyl-CoA + CO2 + FADH2
-
 ?
1.3.99.32 glutaryl-CoA + FAD Desulfococcus multivorans
-
 glutaconyl-CoA + FADH2
-
 ?
1.3.99.32 additional information Desulfococcus multivorans GDHGeo contains highly conserved amino acid residues that are specifically involved in the decarboxylation of the intermediate glutaconyl-CoA ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.8.6 Geobacter metallireducens
-
 an FeIII reducing organism
-
1.3.99.32 Desulfococcus multivorans C3UVB0 a sulfate reducing organism
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.8.6 native enzyme GDHGeo from benzoate-grown cells by two different steps of anion exchange chromatography and gel filtration Geobacter metallireducens
1.3.99.32 recombinant His6-tagged GDHDes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Desulfococcus multivorans

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.8.6 culture condition:benzoate-grown cell
-
 Geobacter metallireducens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.8.6 glutaryl-CoA + FAD via intermediate glutaconyl-CoA Geobacter metallireducens crotonoyl-CoA + CO2 + FADH2
-
 ?
1.3.8.6 glutaryl-CoA + FAD via intermediate glutaconyl-CoA, detection using ferrocenium hexafluorophosphate Geobacter metallireducens crotonoyl-CoA + CO2 + FADH2
-
 ?
1.3.99.32 glutaryl-CoA + FAD
-
 Desulfococcus multivorans glutaconyl-CoA + FADH2
-
 ?
1.3.99.32 additional information GDHGeo contains highly conserved amino acid residues that are specifically involved in the decarboxylation of the intermediate glutaconyl-CoA Desulfococcus multivorans ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.8.6 homotetramer 4 * 43000-44000 Geobacter metallireducens
1.3.99.32 homotetramer 4 * 43000-44000 Desulfococcus multivorans

Synonyms

EC Number Synonyms Comment Organism
1.3.8.6 decarboxylating glutaryl-coenzyme A dehydrogenase
-
 Geobacter metallireducens
1.3.8.6 GDH
-
 Geobacter metallireducens
1.3.8.6 GDHGeo
-
 Geobacter metallireducens
1.3.99.32 GDH
-
 Desulfococcus multivorans
1.3.99.32 GDHDes
-
 Desulfococcus multivorans
1.3.99.32 nondecarboxylating glutaryl-coenzyme A dehydrogenase
-
 Desulfococcus multivorans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.8.6 30
-
 assay at Geobacter metallireducens
1.3.99.32 30
-
 assay at Desulfococcus multivorans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.8.6 7.8
-
 assay at Geobacter metallireducens
1.3.99.32 7.8
-
 assay at Desulfococcus multivorans

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.8.6 FAD GDHGeo contains 0.6-0.7 FAD/monomer Geobacter metallireducens
1.3.99.32 FAD GDHDes contains 0.6-0.7 FAD/monomer Desulfococcus multivorans