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Literature summary extracted from

  • Ding, B.; Schmeling, S.; Fuchs, G.
    Anaerobic metabolism of catechol by the denitrifying bacterium Thauera aromatica - a result of promiscuous enzymes and regulators? (2007), J. Bacteriol., 190, 1620-1630.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.27 expression of the C-terminal His6-tagged protein in Escherichia coli BL21 Thauera aromatica
6.2.1.27 the gene product is expressed as a C-terminal His10-tagged protein in Escherichia coli BL21(DE3) Azoarcus sp.
6.2.1.37 expression of the C-terminal His6-tagged protein in Escherichia coli BL21 Thauera aromatica
6.2.1.37 the gene product is expressed as a C-terminal His10-tagged protein in Escherichia coli BL21(DE3) Azoarcus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.27 0.34
-
 4-hydroxybenzoate pH and temperature not specified in the publication Thauera aromatica
6.2.1.37 0.1
-
 3-hydroxybenzoate pH and temperature not specified in the publication Thauera aromatica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.27 54000
-
 x * 54000, SDS-PAGE Azoarcus sp.
6.2.1.27 57500
-
 x * 57500, SDS-PAGE Thauera aromatica
6.2.1.37 53694
-
 x * 53694, calculated from sequence Azoarcus sp.
6.2.1.37 54000
-
 x * 54000, SDS-PAGE Azoarcus sp.
6.2.1.37 57500
-
 x * 57500, SDS-PAGE Thauera aromatica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.27 ATP + 4-hydroxybenzoate + CoA Azoarcus sp.
-
 AMP + diphosphate + 4-hydroxybenzoyl-CoA
-
 ?
6.2.1.27 ATP + 4-hydroxybenzoate + CoA Thauera aromatica the bifunctional enzyme is also active with 3-hydroxybenzoate (cf. EC 6.2.1.37) AMP + diphosphate + 4-hydroxybenzoyl-CoA
-
 ?
6.2.1.27 ATP + 4-hydroxybenzoate + CoA Azoarcus sp. EbN1
-
 AMP + diphosphate + 4-hydroxybenzoyl-CoA
-
 ?
6.2.1.37 ATP + 3-hydroxybenzoate + CoA Thauera aromatica the bifunctional enzyme is also active with 4-hydroxybenzoate (cf. EC 6.2.1.27) AMP + diphosphate + 3-hydroxybenzoyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.27 Azoarcus sp. Q5P0J2
-
-
6.2.1.27 Azoarcus sp. EbN1 Q5P0J2
-
-
6.2.1.27 Thauera aromatica Q9AJS8
-
-
6.2.1.37 Azoarcus sp. Q5P0J2
-
-
6.2.1.37 Azoarcus sp. EbN1 Q5P0J2
-
-
6.2.1.37 Thauera aromatica Q9AJS8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.27
-
 Thauera aromatica
6.2.1.27
-
 Azoarcus sp.
6.2.1.37
-
 Thauera aromatica
6.2.1.37
-
 Azoarcus sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.2.1.27 cell suspension culture
-
 Thauera aromatica
-
6.2.1.37 cell suspension culture 3-hydroxybenzoate-CoA ligase is detected not only in 3-hydroxybenzoate-grown cells but also in catechol- and protocatechuate-grown cells. The enzyme is not detected in phenol-, 4-hydroxybenzoate-, and benzoate-grown cells Thauera aromatica
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.2.1.27 4.8
-
 pH and temperature not specified in the publication Azoarcus sp.
6.2.1.37 14.3
-
 pH and temperature not specified in the publication Thauera aromatica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.27 ATP + 4-hydroxybenzoate + CoA
-
 Azoarcus sp. AMP + diphosphate + 4-hydroxybenzoyl-CoA
-
 ?
6.2.1.27 ATP + 4-hydroxybenzoate + CoA the bifunctional enzyme is also active with 3-hydroxybenzoate (cf. EC 6.2.1.37) Thauera aromatica AMP + diphosphate + 4-hydroxybenzoyl-CoA
-
 ?
6.2.1.27 ATP + 4-hydroxybenzoate + CoA the bifunctional enzyme is also active with 3-hydroxybenzoate (at 44% of the activity with 4-hydroxybenzoate) (cf. EC 6.2.1.37) Azoarcus sp. AMP + diphosphate + 4-hydroxybenzoyl-CoA
-
 ?
6.2.1.27 ATP + 4-hydroxybenzoate + CoA the bifunctional enzyme is also active with 3-hydroxybenzoate (cf. EC 6.2.1.37). kcat/Km for 4-hydroxybenzoate is 75% compared to the value for 3-hydroxybenzoate Thauera aromatica AMP + diphosphate + 4-hydroxybenzoyl-CoA
-
 ?
6.2.1.27 ATP + 4-hydroxybenzoate + CoA
-
 Azoarcus sp. EbN1 AMP + diphosphate + 4-hydroxybenzoyl-CoA
-
 ?
6.2.1.27 ATP + 4-hydroxybenzoate + CoA the bifunctional enzyme is also active with 3-hydroxybenzoate (at 44% of the activity with 4-hydroxybenzoate) (cf. EC 6.2.1.37) Azoarcus sp. EbN1 AMP + diphosphate + 4-hydroxybenzoyl-CoA
-
 ?
6.2.1.37 ATP + 3-hydroxybenzoate + CoA the bifunctional enzyme is also active with 4-hydroxybenzoate (cf. EC 6.2.1.27) Thauera aromatica AMP + diphosphate + 3-hydroxybenzoyl-CoA
-
 ?
6.2.1.37 ATP + 3-hydroxybenzoate + CoA the bifunctional enzyme is also active with 4-hydroxybenzoate (cf. EC 6.2.1.27). kcat/Km for 4-hydroxybenzoate is 75% compared to the value for 3-hydroxybenzoate Thauera aromatica AMP + diphosphate + 3-hydroxybenzoyl-CoA
-
 ?
6.2.1.37 ATP + 3-hydroxybenzoate + CoA the bifunctional enzyme is also active with 4-hydroxybenzoate, with 2.3fold higher activity compared to 3-hydroxybenzoate (cf. EC 6.2.1.27) Azoarcus sp. AMP + diphosphate + 3-hydroxybenzoyl-CoA
-
 ?
6.2.1.37 ATP + 3-hydroxybenzoate + CoA the bifunctional enzyme is also active with 4-hydroxybenzoate, with 2.3fold higher activity compared to 3-hydroxybenzoate (cf. EC 6.2.1.27) Azoarcus sp. EbN1 AMP + diphosphate + 3-hydroxybenzoyl-CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
6.2.1.27 ? x * 54000, SDS-PAGE Azoarcus sp.
6.2.1.27 ? x * 57500, SDS-PAGE Thauera aromatica
6.2.1.37 ? x * 54000, SDS-PAGE Azoarcus sp.
6.2.1.37 ? x * 57500, SDS-PAGE Thauera aromatica
6.2.1.37 ? x * 53694, calculated from sequence Azoarcus sp.

Synonyms

EC Number Synonyms Comment Organism
6.2.1.27 3-hydroxybenzoate-CoA/4-hydroxybenzoate-CoA ligase bifunctional enzyme EC 6.2.1.27/6.2.1.37 Thauera aromatica
6.2.1.27 4-hydroxybenzoate-CoA ligase
-
 Azoarcus sp.
6.2.1.37 3-hydroxybenzoate-CoA/4-hydroxybenzoate-CoA ligase bifunctional enzyme EC 6.2.1.27/6.2.1.37 Thauera aromatica
6.2.1.37 4-hydroxybenzoate-CoA ligase
-
 Azoarcus sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.2.1.27 24
-
 4-hydroxybenzoate pH and temperature not specified in the publication Thauera aromatica
6.2.1.37 15
-
 3-hydroxybenzoate pH and temperature not specified in the publication Thauera aromatica

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.2.1.27 70
-
 4-hydroxybenzoate pH and temperature not specified in the publication Thauera aromatica
6.2.1.37 150
-
 3-hydroxybenzoate pH and temperature not specified in the publication Thauera aromatica