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Literature summary extracted from

  • Arakawa, T.; Kawano, Y.; Katayama, Y.; Nakayama, H.; Dohmae, N.; Yohda, M.; Odaka, M.
    Structural basis for catalytic activation of thiocyanate hydrolase involving metal-ligated cysteine modification (2009), J. Am. Chem. Soc., 131, 14838-14843.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.5.8 expressed in Escherichia coli BL21(DE3) cells Thiobacillus thioparus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.5.8 hanging drop vapor diffusion method, using 0.1 M potassium phosphate (pH 7.2) containing 2.0 M ammonium sulfate for purified SCNase or 0.1 M potassium phosphate (pH 6.9) containing 1.5 M Na/K tartrate for spontaneously activated SCNase and inactivated SCNase, respectively Thiobacillus thioparus

Organism

EC Number Organism UniProt Comment Textmining
3.5.5.8 Thiobacillus thioparus O66186 subunit beta
-
3.5.5.8 Thiobacillus thioparus O66187 subunit alpha
-
3.5.5.8 Thiobacillus thioparus O66188 subunit gamma
-

Oxidation Stability

EC Number Oxidation Stability Organism
3.5.5.8 when recombinant SCNase, containing gammaCys131-SO2 – and gammaCys133-SO(H) modifications in the cobalt center, is stored at 20°C under aerobic conditions for 4 months, no enzymatic activity is detected Thiobacillus thioparus
3.5.5.8 when recombinant SCNase, containing gammaCys131-SO2- and gammaCys133-SO(H) modifications in the cobalt center, is stored at 20°C under aerobic conditions for 4 months, no enzymatic activity is detected Thiobacillus thioparus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.5.8
-
 Thiobacillus thioparus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.5.8 thiocyanate + H2O
-
 Thiobacillus thioparus carbonyl sulfide + NH3 + OH-
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.5.8 SCNase
-
 Thiobacillus thioparus