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Literature summary extracted from

  • Lee, C.H.; Hsu, K.H.; Wang, S.Y.; Chang, T.T.; Chu, F.H.; Shaw, J.F.
    Cloning and characterization of the lanosterol 14alpha-demethylase gene from Antrodia cinnamomea (2010), J. Agric. Food Chem., 58, 4800-4807.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.154 gene CYP51, DNA and amino acid sequence determination and analysis, functional expression as GST-tagged enzyme in Escherichia coli Taiwanofungus camphoratus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.14.154 itraconazole a fungicide that targets CYP51 and as an inhibitor causes the accumulation of lanosterol and reduced ergosterol biosynthesis in fungi, also induces AcCYP51 expression after 24 h of treatment Taiwanofungus camphoratus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.14.154 61900
-
 x * 87900, full-length recombinant GST-tagged enzyme, SDS-PAGE, x * 84600, mature recombinant GST-tagged enzyme, SDS-PAGE, x * 61900, about, sequence calculation Taiwanofungus camphoratus
1.14.14.154 84600
-
 x * 87900, full-length recombinant GST-tagged enzyme, SDS-PAGE, x * 84600, mature recombinant GST-tagged enzyme, SDS-PAGE, x * 61900, about, sequence calculation Taiwanofungus camphoratus
1.14.14.154 87900
-
 x * 87900, full-length recombinant GST-tagged enzyme, SDS-PAGE, x * 84600, mature recombinant GST-tagged enzyme, SDS-PAGE, x * 61900, about, sequence calculation Taiwanofungus camphoratus

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.154 Taiwanofungus camphoratus A8DBU6 i.e. Antrodia camphorata, gene CYP51
-
1.14.14.154 Taiwanofungus camphoratus TFRIB 470 A8DBU6 i.e. Antrodia camphorata, gene CYP51
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.154 additional information expression analysis at different developmental and growth stages, overview Taiwanofungus camphoratus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.154 lanosterol + [reduced NADPH-hemoprotein reductase] + O2
-
 Taiwanofungus camphoratus 4,4-dimethylcholesta-8,14,24-trienol + formate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.154 lanosterol + [reduced NADPH-hemoprotein reductase] + O2
-
 Taiwanofungus camphoratus TFRIB 470 4,4-dimethylcholesta-8,14,24-trienol + formate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.14.154 ? x * 87900, full-length recombinant GST-tagged enzyme, SDS-PAGE, x * 84600, mature recombinant GST-tagged enzyme, SDS-PAGE, x * 61900, about, sequence calculation Taiwanofungus camphoratus

Synonyms

EC Number Synonyms Comment Organism
1.14.14.154 CYP51
-
 Taiwanofungus camphoratus
1.14.14.154 lanosterol 14alpha-demethylase
-
 Taiwanofungus camphoratus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.14.154 37
-
 assay at Taiwanofungus camphoratus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.154 7.4
-
 assay at Taiwanofungus camphoratus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.14.14.154 Taiwanofungus camphoratus sequence calculation
-
 6.12

Expression

EC Number Organism Comment Expression
1.14.14.154 Taiwanofungus camphoratus transcription of AcCYP51 increases in various culture conditions including adding squalene, lanosterol, itroconazole, and oleic acid as inducers. Itroconazol, a fungicide that targets CYP51 and as an inhibitor causes the accumulation of lanosterol and reduced ergosterol biosynthesis in fungi, also induces AcCYP51 expression after 24 h of treatment up

General Information

EC Number General Information Comment Organism
1.14.14.154 metabolism sterol 14alpha-demethylase is one of the key enzymes for sterol biosynthesis in fungi Taiwanofungus camphoratus