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Literature summary extracted from

  • Virador, V.M.; Reyes Grajeda, J.P.; Blanco-Labra, A.; Mendiola-Olaya, E.; Smith, G.M.; Moreno, A.; Whitaker, J.R.
    Cloning, sequencing, purification, and crystal structure of Grenache (Vitis vinifera) polyphenol oxidase (2010), J. Agric. Food Chem., 58, 1189-1201.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.10.3.1 hanging drop vapor diffusion method, using 30% (w/v) PEG-4000 in 100 mM buffer citrate pH 5.6 and 200 mM ammonium acetate, at 18°C Vitis vinifera

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.1 40000
-
 SDS-PAGE Vitis vinifera
1.10.3.1 56700
-
 calculated from amino acid sequence Vitis vinifera

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.1 Vitis vinifera P93622 cultivar Grenache
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.1 ammonium sulfate precipitation, Superose-R 12 column chromatography, and Sephadex G-75 gel filtration Vitis vinifera

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.10.3.1 fruit
-
 Vitis vinifera
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.1 2 catechol + O2
-
 Vitis vinifera 2 1,2-benzoquinone + 2 H2O
-
 ?
1.10.3.1 4-tert-butylcatechol + O2
-
 Vitis vinifera 4-tert-butyl-1,2-benzoquinone + H2O
-
 ?
1.10.3.1 additional information tyrosine is not a substrate Vitis vinifera ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.10.3.1 1,2-benzenediol:oxygen oxidoreductase
-
 Vitis vinifera
1.10.3.1 catecholase
-
 Vitis vinifera
1.10.3.1 polyphenol oxidase
-
 Vitis vinifera
1.10.3.1 PPO
-
 Vitis vinifera