EC Number | Cloned (Comment) | Organism |
---|---|---|
1.3.5.5 | expression in Escherichia coli | Synechococcus sp. |
1.3.5.5 | expression in Escherichia coli | Gentiana lutea |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.3.5.5 | norflurazon | 0.0001 mM, Vmax values are very little influenced by norflurazon, which reveals a competition between NADP+ or plastoquinone and norflurazon for the cofactor binding site of phytoene desaturase | Synechococcus sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.5.5 | 15-cis-phytoene + 2 plastoquinone | Synechococcus sp. | - |
9,15,9'-tricis-zeta-carotene + 2 plastoquinol | - |
? | |
1.3.5.5 | 15-cis-phytoene + 2 plastoquinone | Gentiana lutea | - |
9,15,9'-tricis-zeta-carotene + 2 plastoquinol | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.5.5 | Gentiana lutea | - |
- |
- |
1.3.5.5 | Synechococcus sp. | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.5.5 | 15-cis-phytoene + 2 decyl-plastoquinone | this quinone derivative is used in the study because the natural C45-plastoquinone is not available commercially | Synechococcus sp. | 9,15,9'-tricis-zeta-carotene + 2 decyl-plastoquinol | - |
? | |
1.3.5.5 | 15-cis-phytoene + 2 decyl-plastoquinone | this quinone derivative is used in the study because the natural C45-plastoquinone is not available commercially. Decyl-plastoquinone is very effective as a cofactor of desaturation. The simultanous presence of NADP+ has no influence on the activity | Gentiana lutea | 9,15,9'-tricis-zeta-carotene + 2 decyl-plastoquinol | - |
? | |
1.3.5.5 | 15-cis-phytoene + 2 decyl-ubiquinone | this quinone derivative is used in the study because the natural C45-ubiquinone is not available commercially | Synechococcus sp. | 9,15,9'-tricis-zeta-carotene + 2 decyl-plastoquinol | - |
? | |
1.3.5.5 | 15-cis-phytoene + 2 decylubiquinone | this quinone derivative is used in the study because the natural C45-ubiquinone is not available commercially | Gentiana lutea | 9,15,9'-tricis-zeta-carotene + 2 decyl-plastoquinol | - |
? | |
1.3.5.5 | 15-cis-phytoene + 2 plastoquinone | - |
Synechococcus sp. | 9,15,9'-tricis-zeta-carotene + 2 plastoquinol | - |
? | |
1.3.5.5 | 15-cis-phytoene + 2 plastoquinone | - |
Gentiana lutea | 9,15,9'-tricis-zeta-carotene + 2 plastoquinol | - |
? | |
1.3.5.5 | additional information | no activity with NADP+ | Gentiana lutea | ? | - |
? | |
1.3.5.5 | additional information | the enzyme also shows activity with NADP+ as cosubstrate, the affinity for NADP+ is lower than for decylplastoquinone or decylubioquinone | Synechococcus sp. | ? | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.3.5.5 | 28 | - |
assay at | Synechococcus sp. |
1.3.5.5 | 28 | - |
assay at | Gentiana lutea |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.3.5.5 | 7.2 | - |
assay at | Synechococcus sp. |
1.3.5.5 | 7.2 | - |
assay at | Gentiana lutea |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.3.5.5 | decyl-plastoquinone | this quinone derivative is used in the study because the natural C45-plastoquinone is not available commercially | Gentiana lutea | |
1.3.5.5 | additional information | the enzyme also shows activity with NADP+ as cosubstrate, the affinity for NADP+ is lower than for decylplastoquinone or decylubioquinone | Synechococcus sp. | |
1.3.5.5 | plastoquinone | decyl-plastoquinone or decyl-ubiquinone is used in the study because the natural C45-plastoquinone is not available commercially. The affinity of the enzyme for plastoquinone is higher than for NADP+ | Synechococcus sp. | |
1.3.5.5 | Ubiquinone-10 | this quinone derivative is used in the study because the natural C45-plastoquinone is not available commercially | Gentiana lutea |