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Literature summary extracted from

  • Tan, Q.Y.; Wang, N.; Yang, H.; Zhang, L.K.; Liu, S.; Chen, L.; Liu, J.; Zhang, L.; Hu, N.N.; Zhao, C.J.; Zhang, J.Q.
    Characterization, stabilization and activity of uricase loaded in lipid vesicles (2010), Int. J. Pharm., 384, 165-172.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.7.3.3 at the physiological pH, significant increase of enzyme activity is found for the uricase entrapped in the lipid vesicles (1.8times that of free uricase) at their respective optimum pH. Free uricase shows rapid decrease in its enzymatic activity with a half life of less than 20 min when incubated with trypsin. Uricase entrapped in the lipid vesicles gradually loses its activity but still 50% of the original activity remains after 60 min (remaining activity is 7.32% in case of free uricase) Cyberlindnera jadinii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.3.3 beta-mercaptoethanol about 60% residual activity after 1 h incubation with 0.5 mM beta-mercaptoethanol at pH 8.5 and 25°C Cyberlindnera jadinii
1.7.3.3 D-sorbitol about 70% residual activity after 1 h incubation with 0.5 mM D-sorbitol at pH 8.5 and 25°C Cyberlindnera jadinii
1.7.3.3 sodium deoxycholate about 90% residual activity after 1 h incubation with 0.5 mM sodium deoxycholate at pH 8.5 and 25°C Cyberlindnera jadinii

Organism

EC Number Organism UniProt Comment Textmining
1.7.3.3 Cyberlindnera jadinii
-
-
-

Storage Stability

EC Number Storage Stability Organism
1.7.3.3 4°C, free uricase in borate buffer (pH 8.5), 28 days, 70% loss of activity Cyberlindnera jadinii
1.7.3.3 4°C, uricase in lipid vesicles in borate buffer (pH 8.5), 28 days, no loss of activity Cyberlindnera jadinii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.3.3 urate + O2 + H2O
-
 Cyberlindnera jadinii 5-hydroxyisourate + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.7.3.3 Uox
-
 Cyberlindnera jadinii
1.7.3.3 uricase
-
 Cyberlindnera jadinii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.7.3.3 37
-
-
 Cyberlindnera jadinii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.7.3.3 20 70 free uricase shows at least 50% relative activity between 20 and 70°C Cyberlindnera jadinii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.7.3.3 55
-
 uricase entrapped in lipid vesicles at enzyme concentrations of 0.005 and 0.1 mg/ml shows no loss of activity after 5 h at 55°C. Uricase entrapped in lipid vesicles at enzyme concentrations of 0.01 mg/ml shows about 35% loss of activity after 5 h at 55°C. Uricase entrapped in lipid vesicles at enzyme concentrations of 0.005 mg/ml shows about 50% loss of activity after 5 h at 55°C. The free uricase at 0.005 mg/ml is rapidly deactivated to about 30% of the initial activity within an incubation time of 2 h, while more than 70% of the initial enzyme activity remains for the uricase at 0.1 mg/ml in the identical incubation time Cyberlindnera jadinii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.3.3 8.5
-
-
 Cyberlindnera jadinii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.7.3.3 6.5 9.5 free uricase shows at least 50% relative activity between pH 6.5 and 9.5, around pH 7.5, free uricase remains 81.16% of its maximum activity, while the uricase loaded in the lipid vesicles remains almost the same high activity (178.26%) as its optimum activity (179.72%) Cyberlindnera jadinii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.7.3.3 7 9.5 in case of uricase entrapped in lipid vesicles, the remaining activity keeps more than 90% during the pH of 7.0-9.5, and the maximum remaining activity is 98.04% at pH 8.0 when incubated at 40°C or 40 min. For the free uricase, the maximum remaining activity is 86.59% at pH 8.5 when incubated at 40°C or 40 min Cyberlindnera jadinii