Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Li, F.; Wu, Q.Y.; Sun, Y.L.; Ma, N.N.; Wang, X.Y.; Meng, Q.W.
    Evidence that the amino acid residue Cys117 of chloroplastic monodehydroascorbate reductase is involved in its activity and structural stability (2010), Int. J. Biol. Macromol., 46, 350-355.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.6.5.4 expressed as GST-tagged enzyme in Escherichia coli BL21 cells Solanum lycopersicum

Protein Variants

EC Number Protein Variants Comment Organism
1.6.5.4 C117A the binding affinity of NADH in the mutant MDAR is lower than that of wild type MDAR as indicated by about 1.5-3fold increase of the Km value for NADH, the mutant is less thermo-stable than the wild type enzyme Solanum lycopersicum
1.6.5.4 C117S the binding affinity of NADH in the mutant MDAR is lower than that of wild type MDAR as indicated by about 1.5-3fold increase of the Km value for NADH, the mutant is less thermo-stable than the wild type enzyme Solanum lycopersicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.5.4 0.00144
-
 monodehydroascorbate native wild type enzyme, in 50 mM Tris-HCl (pH 7.5), at 25°C Solanum lycopersicum
1.6.5.4 0.0015
-
 monodehydroascorbate recombinant wild type enzyme, in 50 mM Tris-HCl (pH 7.5), at 25°C Solanum lycopersicum
1.6.5.4 0.00151
-
 monodehydroascorbate mutant enzyme C117S, in 50 mM Tris-HCl (pH 7.5), at 25°C Solanum lycopersicum
1.6.5.4 0.00155
-
 monodehydroascorbate mutant enzyme C117A, in 50 mM Tris-HCl (pH 7.5), at 25°C Solanum lycopersicum
1.6.5.4 0.00621
-
 NADH recombinant wild type enzyme, in 50 mM Tris-HCl (pH 7.5), at 25°C Solanum lycopersicum
1.6.5.4 0.0063
-
 NADH native wild type enzyme, in 50 mM Tris-HCl (pH 7.5), at 25°C Solanum lycopersicum
1.6.5.4 0.00962
-
 NADH mutant enzyme C117S, in 50 mM Tris-HCl (pH 7.5), at 25°C Solanum lycopersicum
1.6.5.4 0.01461
-
 NADH mutant enzyme C117A, in 50 mM Tris-HCl (pH 7.5), at 25°C Solanum lycopersicum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.6.5.4 chloroplast
-
 Solanum lycopersicum 9507
-

Organism

EC Number Organism UniProt Comment Textmining
1.6.5.4 Solanum lycopersicum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.5.4 glutathione Sepharose column chromatography Solanum lycopersicum

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.6.5.4 using 0.8 M guanidine-HCl at 25°C for 24 h Solanum lycopersicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.5.4 NADH + monodehydroascorbate
-
 Solanum lycopersicum NAD+ + ascorbate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.6.5.4 MDAR
-
 Solanum lycopersicum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.6.5.4 35 62 the activity of wild type MDAR has no much change after heat treatment at temperatures below 35°C, steep decrease of activity is observed between 35 and 55°C, and a complete loss in activity occurs above 62°C. The midpoint of thermal inactivation of wild type MDAR is about 52.1°C Solanum lycopersicum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.6.5.4 62.67
-
 NADH mutant enzyme C117A, in 50 mM Tris-HCl (pH 7.5), at 25°C Solanum lycopersicum
1.6.5.4 89.89
-
 NADH mutant enzyme C117S, in 50 mM Tris-HCl (pH 7.5), at 25°C Solanum lycopersicum
1.6.5.4 121.4
-
 NADH recombinant wild type enzyme, in 50 mM Tris-HCl (pH 7.5), at 25°C Solanum lycopersicum
1.6.5.4 126.6
-
 NADH native wild type enzyme, in 50 mM Tris-HCl (pH 7.5), at 25°C Solanum lycopersicum

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.5.4 NADH
-
 Solanum lycopersicum

General Information

EC Number General Information Comment Organism
1.6.5.4 physiological function MDARis a key enzyme in the ascorbate-glutathione cycle, MDAR is crucial for ascorbate regeneration and essential for maintaining the reduced pool of ascorbate Solanum lycopersicum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.6.5.4 4290
-
 NADH mutant enzyme C117A, in 50 mM Tris-HCl (pH 7.5), at 25°C Solanum lycopersicum
1.6.5.4 9360
-
 NADH mutant enzyme C117S, in 50 mM Tris-HCl (pH 7.5), at 25°C Solanum lycopersicum
1.6.5.4 19270
-
 NADH recombinant wild type enzyme, in 50 mM Tris-HCl (pH 7.5), at 25°C Solanum lycopersicum
1.6.5.4 20420
-
 NADH native wild type enzyme, in 50 mM Tris-HCl (pH 7.5), at 25°C Solanum lycopersicum