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Literature summary extracted from
- Phosphorylation regulates copper-responsive trafficking of the Menkes copper transporting P-type ATPase (2009), Int. J. Biochem. Cell Biol., 41, 2403-2412.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 7.2.2.9 | expression of mutant enzymes in MDCK cells and in CHO cells | Homo sapiens |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 7.2.2.9 | S1432A | site-directed mutagenesis of a constitutively phosphorylated site, mutation leads to mislocation of the enzyme in presence of Cu2+ | Homo sapiens |
| 7.2.2.9 | S1469A | site-directed mutagenesis of a copper-responsive phosphorylation site, mutation leads to mislocation of the enzyme in presence of Cu2+ | Homo sapiens |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 7.2.2.9 | Golgi membrane | - |
Cricetulus griseus | 139 | - |
| 7.2.2.9 | Golgi membrane | - |
Homo sapiens | 139 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.2.2.9 | Cu2+ | copper stimulates both trafficking and phosphorylation of ATP7A | Cricetulus griseus |  |
| 7.2.2.9 | Cu2+ | copper stimulates both trafficking and phosphorylation of ATP7A | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.2.2.9 | ATP + H2O + Cu2+/in | Cricetulus griseus | copper stimulates both trafficking and phosphorylation of ATP7A | ADP + phosphate + Cu2+/out | - |
? | |
| 7.2.2.9 | ATP + H2O + Cu2+/in | Homo sapiens | copper stimulates both trafficking and phosphorylation of ATP7A | ADP + phosphate + Cu2+/out | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 7.2.2.9 | Cricetulus griseus | - |
gene ATP7A | - |
| 7.2.2.9 | Homo sapiens | - |
gene ATP7A | - |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 7.2.2.9 | phosphoprotein | copper stimulates both trafficking and phosphorylation of ATP7A, identification of eight in vivo phosphorylation sites in the hamster ATP7A, all clustered within the N- and C-terminal cytosolic domains, C- and N-terminal phosphorylation sites, overview | Cricetulus griseus |
| 7.2.2.9 | phosphoprotein | copper stimulates both trafficking and phosphorylation of ATP7A, identification of twenty in vivo phosphorylation sites in the human ATP7A, all clustered within the N- and C-terminal cytosolic domains, eight sites are copper-responsive and hence candidates for regulating copper-responsive trafficking or catalytic activity. A constitutively phosphorylated site is Ser1432, a copper-responsive phosphorylation site Ser1469. Other C-terminal phosphorylation sites are serine residues 1463, 1466, 1469, 1473, 1476 and 1480, C- and N-termninal phosphorylation sites, overview | Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 7.2.2.9 | CHO-CUR3 cell | - |
Cricetulus griseus | - |
| 7.2.2.9 | CHO-K1 cell | - |
Cricetulus griseus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.2.2.9 | ATP + H2O + Cu2+/in | - |
Cricetulus griseus | ADP + phosphate + Cu2+/out | - |
? | |
| 7.2.2.9 | ATP + H2O + Cu2+/in | - |
Homo sapiens | ADP + phosphate + Cu2+/out | - |
? | |
| 7.2.2.9 | ATP + H2O + Cu2+/in | copper stimulates both trafficking and phosphorylation of ATP7A | Cricetulus griseus | ADP + phosphate + Cu2+/out | - |
? | |
| 7.2.2.9 | ATP + H2O + Cu2+/in | copper stimulates both trafficking and phosphorylation of ATP7A | Homo sapiens | ADP + phosphate + Cu2+/out | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 7.2.2.9 | More | peptide mapping and identification of putative Cu-responsive peptides, overview | Cricetulus griseus |
| 7.2.2.9 | More | peptide mapping and identification of putative Cu-responsive peptides, overview | Homo sapiens |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 7.2.2.9 | ATP7A | - |
Cricetulus griseus |
| 7.2.2.9 | ATP7A | - |
Homo sapiens |
| 7.2.2.9 | copper transporting P-type ATPase | - |
Cricetulus griseus |
| 7.2.2.9 | Cu-translocating P-type ATPase | - |
Cricetulus griseus |
| 7.2.2.9 | Cu-translocating P-type ATPase | - |
Homo sapiens |
| 7.2.2.9 | Menkes copper transporting P-type ATPase | - |
Homo sapiens |
| 7.2.2.9 | MNK | - |
Homo sapiens |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 7.2.2.9 | additional information | specific serine residues in ATP7A regulates its sub-cellular localization and hence function and will facilitate identification of the kinases and signaling pathways involved in regulating this pivotal copper transporter | Cricetulus griseus |
| 7.2.2.9 | additional information | specific serine residues in ATP7A regulates its sub-cellular localization and hence function and will facilitate identification of the kinases and signaling pathways involved in regulating this pivotal copper transporter. C-terminal MNK phosphorylation is essential for copper-responsive trafficking | Homo sapiens |
| 7.2.2.9 | physiological function | Menkes copper-translocating P-type ATPase, ATP7A, is a critical copper transport protein functioning in systemic copper absorption and supply of copper to cuproenzymes in the secretory pathway. Mutations in ATP7A can lead to the usually lethal Menkes disease. ATP7A function is regulated by copper-responsive trafficking between the trans-Golgi Network and the plasma membrane | Homo sapiens |
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