EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.3.99.30 | NAD+ | more than 2fold stimulation of activity compared to control, assay with supernatant of Escherichia coli recombinantly expressing phytoene desaturase from Neurospora crassa | Neurospora crassa | |
1.3.99.30 | NADP+ | stimulation is lower than with NAD+, assay with supernatant of Escherichia coli recombinantly expressing phytoene desaturase from Neurospora crassa | Neurospora crassa |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.3.99.30 | expression in Escherichia coli | Neurospora crassa |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.3.99.30 | 62000 | - |
x * 62000, SDS-PAGE | Neurospora crassa |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.99.30 | 15-cis-phytoene + 5 acceptor | Neurospora crassa | the enzyme is involved in the carotenoid biosynthesis pathway to beta-carotene and torulene | all-trans-3,4-didehydrolycopene + 5 reduced acceptor | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.99.30 | Neurospora crassa | - |
- |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.3.99.30 | 15-cis-phytoene + 5 acceptor = all-trans-3,4-didehydrolycopene + 5 reduced acceptor | overall reaction | Neurospora crassa |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.99.30 | 1-hydroxylycopene + acceptor | - |
Neurospora crassa | 1-hydroxy-3',4'-didehydrolycopene + reduced acceptor | - |
? | |
1.3.99.30 | 1-hydroxyneurosporene + acceptor | - |
Neurospora crassa | 1-hydroxylycopene + reduced acceptor | - |
? | |
1.3.99.30 | 15-cis-phytoene + 5 acceptor | - |
Neurospora crassa | all-trans-3,4-didehydrolycopene + 5 reduced acceptor | - |
? | |
1.3.99.30 | 15-cis-phytoene + 5 acceptor | the enzyme is involved in the carotenoid biosynthesis pathway to beta-carotene and torulene | Neurospora crassa | all-trans-3,4-didehydrolycopene + 5 reduced acceptor | - |
? | |
1.3.99.30 | all-trans-lycopene + acceptor | - |
Neurospora crassa | all-trans-3,4-didehydrolycopene + reduced acceptor | - |
? | |
1.3.99.30 | all-trans-neurosporene + 2 acceptor | - |
Neurospora crassa | all-trans-3,4-didehydrolycopene + 2 reduced acceptor | - |
? | |
1.3.99.30 | all-trans-phytofluene + 4 acceptor | - |
Neurospora crassa | all-trans-3,4-didehydrolycopene + 4 reduced acceptor | - |
? | |
1.3.99.30 | all-trans-zeta-carotene + 3 acceptor | - |
Neurospora crassa | all-trans-3,4-didehydrolycopene + 3 reduced acceptor | - |
? | |
1.3.99.30 | additional information | gamma-carotene and 1,1'-dihydroxylycopene are not accepted as substrate | Neurospora crassa | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.3.99.30 | ? | x * 62000, SDS-PAGE | Neurospora crassa |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.3.99.30 | Al-1 | - |
Neurospora crassa |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.3.99.30 | 30 | - |
assay at | Neurospora crassa |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.3.99.30 | 8 | - |
assay at | Neurospora crassa |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.3.99.30 | additional information | addition of FAD results in product formation close to that of the control without additions | Neurospora crassa |