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Literature summary extracted from

  • Cardenas-Lopez, J.; Haard, N.
    Identification of a cysteine proteinase from Jumbo squid (Dosidicus gigas) hepatopancreas as cathepsin L (2009), Food Chem., 112, 442-447.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.15 chymostatin 32.24% residual activity at 0.2 mM Dosidicus gigas
3.4.22.15 E-64 i.e. L-trans-epoxysuccinyl-leucylamide-(4-guanido)-butane, 24.76% residual activity at 1 mg/ml Dosidicus gigas
3.4.22.15 leupeptin 11.06% residual activity at 0.001 mg/ml Dosidicus gigas
3.4.22.15 pefabloc 78.43% residual activity at 2 mg/ml Dosidicus gigas
3.4.22.15 pepstatin 58.06% residual activity at 0.001 mg/ml Dosidicus gigas
3.4.22.15 Soybean trypsin inhibitor 63.12% residual activity at 0.05 mg/ml Dosidicus gigas
3.4.22.15 tosyl phenylalanyl chloromethylketone 66.63% residual activity at 0.1 mg/ml Dosidicus gigas

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.15 23790
-
 MALDI-TOF mass spectrometry Dosidicus gigas
3.4.22.15 24000
-
 SDS-PAGE Dosidicus gigas

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.15 azocasein + H2O Dosidicus gigas
-
 ?
-
 ?
3.4.22.15 casein + H2O Dosidicus gigas
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.15 Dosidicus gigas
-
 jumbo squid
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.15 ammonium sulfate precipitation, column chromatography, Toyopearl HW-55 F gel filtration Dosidicus gigas

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.15 hepatopancreas
-
 Dosidicus gigas
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.22.15 additional information
-
 the crude extract has a specific activity of 110 units/mg protein, the 15fold purified enzyme has a specific activity of 1644 units/mg protein at pH 4.5 and 55°C, one enzyme unit is defined as the absorbance at 380 nm given by 0.001 mM of free 7-amino-4-methylcoumarin per min Dosidicus gigas

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.15 azocasein + H2O
-
 Dosidicus gigas ?
-
 ?
3.4.22.15 benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-trifluoromethylcoumarin + H2O cathepsin L specific synthetic substrate Dosidicus gigas benzyloxycarbonyl-L-Phe-L-Arg + 7-amino-4-trifluoromethylcoumarin
-
 ?
3.4.22.15 casein + H2O
-
 Dosidicus gigas ?
-
 ?
3.4.22.15 additional information the cathepsin B and H specific synthetic substrates benzyloxycarbonyl-L-Ala-L-Ala-7-amido-4-trifluoromethylcoumarin and L-Arg-7-amido-4-methylcoumarin do not show any hydrolysis with the partially purified enzyme Dosidicus gigas ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.15 ? x * 24000, SDS-PAGE Dosidicus gigas

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.22.15 55
-
 with the substrate benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-trifluoromethylcoumarin Dosidicus gigas

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.22.15 40 70 about 60% of the maximum activity at 40°C and at 70°C with the substrate benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-trifluoromethylcoumarin Dosidicus gigas

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.15 4.5
-
 with the substrate benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-trifluoromethylcoumarin Dosidicus gigas

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.22.15 3.5 6 about 70% of the maximum activity at pH 3.5 and about 45% of the maximum activity at pH 6.0 with the substrate benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-trifluoromethylcoumarin Dosidicus gigas