EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.1.210 | expression in Escherichia coli in an active form | Rhodobacter capsulatus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.210 | 0.0266 | - |
1'-hydroxydemethylspheroidene | pH 8.0, 28°C | Rhodobacter capsulatus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.1.1.210 | 43000 | - |
x * 43000, SDS-PAGE | Rhodobacter capsulatus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.210 | S-adenosyl-L-methionine + 1'-hydroxydemethylspheroidene | Rhodobacter capsulatus | the enzyme is involved in the biosynthesis of spheroidene and spirilloxanthin, essential components of the photosynthetic apparatus | S-adenosyl-L-homocysteine + 1'-hydroxyspheroidene | - |
? | |
2.1.1.210 | S-adenosyl-L-methionine + 1,1'-dihydroxyneurosporene | Rhodobacter capsulatus | the enzyme is involved in the biosynthesis of spheroidene and spirilloxanthin, essential components of the photosynthetic apparatus | S-adenosyl-L-homocysteine + 1-methoxy-1'-hydroxyneurosporene | - |
? | |
2.1.1.210 | S-adenosyl-L-methionine + 1-hydroxyneurosporene | Rhodobacter capsulatus | the enzyme is involved in the biosynthesis of spheroidene and spirilloxanthin, essential components of the photosynthetic apparatus | S-adenosyl-L-homocysteine + 1-methoxyneurosporene | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.210 | Rhodobacter capsulatus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.1.210 | - |
Rhodobacter capsulatus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.210 | additional information | the enzyme converts not only all possible 1-hydroxycarotenoids in the spheroidene/1'-hydroxyspheroidene biosynthetic pathway of Rhodobacter capsulatus but also carotenoid intermediates leading to the formation of spirilloxanthin in a pathway which is absent in Rhodobacter capsulatus but present in related species. Hydroxycarotenoids not accepted by CrtF are 1'-hydroxy-3,4-didehydrolycopene, 1-hydroxy-zeta-carotene, 1,1'-dihydroxy-zeta-carotene both without the 7,8- and 7',8'-double bond and cyclic zeaxanthin. From all these complementation results the structural requirements of a substrate for CrtF can be established. The 1-hydroxy-carotenoid should be acyclic with a 5,6-double bond in conjugation with the central polyene chain. A 3,4-double bond is optional. The other end group can be quite variable without a negative impact on methylation, provided that it is not a fully desaturated psi-end group | Rhodobacter capsulatus | ? | - |
? | |
2.1.1.210 | S-adenosyl-L-methionine + 1'-hydroxydemethylspheroidene | - |
Rhodobacter capsulatus | S-adenosyl-L-homocysteine + 1'-hydroxyspheroidene | - |
? | |
2.1.1.210 | S-adenosyl-L-methionine + 1'-hydroxydemethylspheroidene | the enzyme is involved in the biosynthesis of spheroidene and spirilloxanthin, essential components of the photosynthetic apparatus | Rhodobacter capsulatus | S-adenosyl-L-homocysteine + 1'-hydroxyspheroidene | - |
? | |
2.1.1.210 | S-adenosyl-L-methionine + 1,1'-dihydroxy-3,4,3',4'-tetradehydrolycopene | not involved in the carotenogenic pathway of Rhodobacter capsulatus | Rhodobacter capsulatus | S-adenosyl-L-homocysteine + 1,1'-dimethoxy-3,4,3',4'-tetradehydrolycopene | - |
? | |
2.1.1.210 | S-adenosyl-L-methionine + 1,1'-dihydroxy-3,4-didehydrolycopene | not involved in the carotenogenic pathway of Rhodobacter capsulatus | Rhodobacter capsulatus | S-adenosyl-L-homocysteine + 1,1'-dimethoxy-3,4-didehydrolycopene | - |
? | |
2.1.1.210 | S-adenosyl-L-methionine + 1,1'-dihydroxylycopene | not involved in the carotenogenic pathway of Rhodobacter capsulatus | Rhodobacter capsulatus | S-adenosyl-L-homocysteine + 1,1'-dimethoxylycopene | - |
? | |
2.1.1.210 | S-adenosyl-L-methionine + 1,1'-dihydroxyneurosporene | - |
Rhodobacter capsulatus | S-adenosyl-L-homocysteine + 1-methoxy-1'-hydroxyneurosporene | - |
? | |
2.1.1.210 | S-adenosyl-L-methionine + 1,1'-dihydroxyneurosporene | the enzyme is involved in the biosynthesis of spheroidene and spirilloxanthin, essential components of the photosynthetic apparatus | Rhodobacter capsulatus | S-adenosyl-L-homocysteine + 1-methoxy-1'-hydroxyneurosporene | - |
? | |
2.1.1.210 | S-adenosyl-L-methionine + 1-hydroxy-3,4-didehydrolycopene | not involved in the carotenogenic pathway of Rhodobacter capsulatus | Rhodobacter capsulatus | S-adenosyl-L-homocysteine + 1-methoxy-3,4-didehydrolycopene | - |
? | |
2.1.1.210 | S-adenosyl-L-methionine + 1-hydroxylycopene | not involved in the carotenogenic pathway of Rhodobacter capsulatus | Rhodobacter capsulatus | S-adenosyl-L-homocysteine + 1-methoxylycopene | - |
? | |
2.1.1.210 | S-adenosyl-L-methionine + 1-hydroxyneurosporene | - |
Rhodobacter capsulatus | S-adenosyl-L-homocysteine + 1-methoxyneurosporene | - |
? | |
2.1.1.210 | S-adenosyl-L-methionine + 1-hydroxyneurosporene | the enzyme is involved in the biosynthesis of spheroidene and spirilloxanthin, essential components of the photosynthetic apparatus | Rhodobacter capsulatus | S-adenosyl-L-homocysteine + 1-methoxyneurosporene | - |
? | |
2.1.1.210 | S-adenosyl-L-methionine + demethylspheroidene | - |
Rhodobacter capsulatus | S-adenosyl-L-homocysteine + spheroidene | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.1.1.210 | ? | x * 43000, SDS-PAGE | Rhodobacter capsulatus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.1.1.210 | 1-HO-carotenoid methylase | - |
Rhodobacter capsulatus |
2.1.1.210 | 1-hydroxycarotenoid methylase | - |
Rhodobacter capsulatus |
2.1.1.210 | 1-hydroxycarotenoid O-methylase | - |
Rhodobacter capsulatus |
2.1.1.210 | CrtF | - |
Rhodobacter capsulatus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.1.1.210 | 28 | - |
assay at | Rhodobacter capsulatus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.1.1.210 | 8 | - |
- |
Rhodobacter capsulatus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.210 | S-adenosyl-L-methionine | - |
Rhodobacter capsulatus |