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Literature summary extracted from

  • Barquera, B.; Hase, C.C.; Gennis, R.B.
    Expression and mutagenesis of the NqrC subunit of the NQR respiratory Na+ pump from Vibrio cholerae with covalently attached FMN (2001), FEBS Lett., 492, 45-49.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.2.1.1 subunit NqrC is expressed in Vibrio cholerae and in Escherichia coli TOP10 cells. Subunit NqrB expression is toxic for Vibrio cholerae Vibrio cholerae serotype O1

Protein Variants

EC Number Protein Variants Comment Organism
7.2.1.1 H216L the subunit NqrC mutant contains 0.2-0.3 mol of FMN per mol of protein, the mutation reduces the FMN content (30%) of the isolated subunit NqrC Vibrio cholerae serotype O1
7.2.1.1 T225L no covalent flavin is detected when threonine-225 is replaced by leucine, the mutation eliminates flavin binding by subunit NqrC Vibrio cholerae serotype O1

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
7.2.1.1 27600
-
 subunit NqrC, calculated from amino acid sequence Vibrio cholerae serotype O1
7.2.1.1 32000
-
 recombinant subunit NqrC, SDS-PAGE Vibrio cholerae serotype O1

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.2.1.1 NADH + H+ + ubiquinone + n Na+/in Vibrio cholerae serotype O1
-
 NAD+ + ubiquinol + n Na+/out
-
 ?
7.2.1.1 NADH + H+ + ubiquinone + n Na+/in Vibrio cholerae serotype O1 ATCC 39541
-
 NAD+ + ubiquinol + n Na+/out
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
7.2.1.1 Vibrio cholerae serotype O1 A5F5X1 and A5F5X0 and A5F5Y7 and A5F5Y6 and A5F5Y3 and A5F5Y4 A5F5X1: subunit NqrA, A5F5X0: subunit NqrB, A5F5Y7: subunit NqrC, A5F5Y6: subunit NqrD; A5F5Y3: subunit NqrE, A5F5Y4: subunit NqrF. The enzyme consists of six subunits encoded by the NQR operon.
-
7.2.1.1 Vibrio cholerae serotype O1 ATCC 39541 A5F5X1 and A5F5X0 and A5F5Y7 and A5F5Y6 and A5F5Y3 and A5F5Y4 A5F5X1: subunit NqrA, A5F5X0: subunit NqrB, A5F5Y7: subunit NqrC, A5F5Y6: subunit NqrD; A5F5Y3: subunit NqrE, A5F5Y4: subunit NqrF. The enzyme consists of six subunits encoded by the NQR operon.
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.2.1.1 Ni-NTA column chromatography and Sephadex G-25 gel filtration Vibrio cholerae serotype O1

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.2.1.1 NADH + H+ + ubiquinone + n Na+/in
-
 Vibrio cholerae serotype O1 NAD+ + ubiquinol + n Na+/out
-
 ?
7.2.1.1 NADH + H+ + ubiquinone + n Na+/in
-
 Vibrio cholerae serotype O1 ATCC 39541 NAD+ + ubiquinol + n Na+/out
-
 ?

Synonyms

EC Number Synonyms Comment Organism
7.2.1.1 Na+-NQR
-
 Vibrio cholerae serotype O1
7.2.1.1 Na+-translocating NADH:quinone oxidoreductase
-
 Vibrio cholerae serotype O1

Cofactor

EC Number Cofactor Comment Organism Structure
7.2.1.1 FAD
-
 Vibrio cholerae serotype O1
7.2.1.1 FMN subunits NqrB and NqrC contain covalently bound FMN, the wild type subunit NqrC contains approximately 1 mol of FMN per mol of protein Vibrio cholerae serotype O1
7.2.1.1 NADH
-
 Vibrio cholerae serotype O1
7.2.1.1 riboflavin
-
 Vibrio cholerae serotype O1
7.2.1.1 [2Fe-2S]-center
-
 Vibrio cholerae serotype O1