BRENDA - Enzyme Database

Expression and mutagenesis of the NqrC subunit of the NQR respiratory Na+ pump from Vibrio cholerae with covalently attached FMN

Barquera, B.; Hase, C.C.; Gennis, R.B.; FEBS Lett. 492, 45-49 (2001)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
7.2.1.1
subunit NqrC is expressed in Vibrio cholerae and in Escherichia coli TOP10 cells. Subunit NqrB expression is toxic for Vibrio cholerae
Vibrio cholerae
Engineering
EC Number
Amino acid exchange
Commentary
Organism
7.2.1.1
H216L
the subunit NqrC mutant contains 0.2-0.3 mol of FMN per mol of protein, the mutation reduces the FMN content (30%) of the isolated subunit NqrC
Vibrio cholerae
7.2.1.1
T225L
no covalent flavin is detected when threonine-225 is replaced by leucine, the mutation eliminates flavin binding by subunit NqrC
Vibrio cholerae
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
7.2.1.1
27600
-
subunit NqrC, calculated from amino acid sequence
Vibrio cholerae
7.2.1.1
32000
-
recombinant subunit NqrC, SDS-PAGE
Vibrio cholerae
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
7.2.1.1
NADH + H+ + ubiquinone + n Na+/in
Vibrio cholerae
-
NAD+ + ubiquinol + n Na+/out
-
-
?
7.2.1.1
NADH + H+ + ubiquinone + n Na+/in
Vibrio cholerae O395-N1
-
NAD+ + ubiquinol + n Na+/out
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
7.2.1.1
Vibrio cholerae
-
-
-
7.2.1.1
Vibrio cholerae O395-N1
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
7.2.1.1
Ni-NTA column chromatography and Sephadex G-25 gel filtration
Vibrio cholerae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7.2.1.1
NADH + H+ + ubiquinone + n Na+/in
-
711997
Vibrio cholerae
NAD+ + ubiquinol + n Na+/out
-
-
-
?
7.2.1.1
NADH + H+ + ubiquinone + n Na+/in
-
711997
Vibrio cholerae O395-N1
NAD+ + ubiquinol + n Na+/out
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
7.2.1.1
FAD
-
Vibrio cholerae
7.2.1.1
FMN
subunits NqrB and NqrC contain covalently bound FMN, the wild type subunit NqrC contains approximately 1 mol of FMN per mol of protein
Vibrio cholerae
7.2.1.1
NADH
-
Vibrio cholerae
7.2.1.1
riboflavin
-
Vibrio cholerae
7.2.1.1
[2Fe-2S]-center
-
Vibrio cholerae
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
7.2.1.1
subunit NqrC is expressed in Vibrio cholerae and in Escherichia coli TOP10 cells. Subunit NqrB expression is toxic for Vibrio cholerae
Vibrio cholerae
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
7.2.1.1
FAD
-
Vibrio cholerae
7.2.1.1
FMN
subunits NqrB and NqrC contain covalently bound FMN, the wild type subunit NqrC contains approximately 1 mol of FMN per mol of protein
Vibrio cholerae
7.2.1.1
NADH
-
Vibrio cholerae
7.2.1.1
riboflavin
-
Vibrio cholerae
7.2.1.1
[2Fe-2S]-center
-
Vibrio cholerae
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
7.2.1.1
H216L
the subunit NqrC mutant contains 0.2-0.3 mol of FMN per mol of protein, the mutation reduces the FMN content (30%) of the isolated subunit NqrC
Vibrio cholerae
7.2.1.1
T225L
no covalent flavin is detected when threonine-225 is replaced by leucine, the mutation eliminates flavin binding by subunit NqrC
Vibrio cholerae
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
7.2.1.1
27600
-
subunit NqrC, calculated from amino acid sequence
Vibrio cholerae
7.2.1.1
32000
-
recombinant subunit NqrC, SDS-PAGE
Vibrio cholerae
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
7.2.1.1
NADH + H+ + ubiquinone + n Na+/in
Vibrio cholerae
-
NAD+ + ubiquinol + n Na+/out
-
-
?
7.2.1.1
NADH + H+ + ubiquinone + n Na+/in
Vibrio cholerae O395-N1
-
NAD+ + ubiquinol + n Na+/out
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
7.2.1.1
Ni-NTA column chromatography and Sephadex G-25 gel filtration
Vibrio cholerae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7.2.1.1
NADH + H+ + ubiquinone + n Na+/in
-
711997
Vibrio cholerae
NAD+ + ubiquinol + n Na+/out
-
-
-
?
7.2.1.1
NADH + H+ + ubiquinone + n Na+/in
-
711997
Vibrio cholerae O395-N1
NAD+ + ubiquinol + n Na+/out
-
-
-
?