BRENDA - Enzyme Database

Cloning and sequencing of four structural genes for the Na+-translocating NADH-ubiquinone oxidoreductase of Vibrio alginolyticus

Beattie, P.; Tan, K.; Bourne, R.M.; Leach, D.; Rich, P.R.; Ward, F.B.; FEBS Lett. 356, 333-338 (1994)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
7.2.1.1
2-n-heptyl-4-hydroxyquinoline N-oxide
-
Vibrio alginolyticus
7.2.1.1
Ag+
-
Vibrio alginolyticus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
7.2.1.1
membrane
-
Vibrio alginolyticus
16020
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
7.2.1.1
22602
-
1 * 48622 + 1 * 46809 + 1 * 27672 + 1 * 22602, subunits NqrA, NqrB, NqrC and NqrD, calculated from amino acid sequence
Vibrio alginolyticus
7.2.1.1
27672
-
1 * 48622 + 1 * 46809 + 1 * 27672 + 1 * 22602, subunits NqrA, NqrB, NqrC and NqrD, calculated from amino acid sequence
Vibrio alginolyticus
7.2.1.1
46809
-
1 * 48622 + 1 * 46809 + 1 * 27672 + 1 * 22602, subunits NqrA, NqrB, NqrC and NqrD, calculated from amino acid sequence
Vibrio alginolyticus
7.2.1.1
48622
-
1 * 48622 + 1 * 46809 + 1 * 27672 + 1 * 22602, subunits NqrA, NqrB, NqrC and NqrD, calculated from amino acid sequence
Vibrio alginolyticus
7.2.1.1
254000
-
gel filtration
Vibrio alginolyticus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
7.2.1.1
deamino-NADH + H+ + ubiquinone + n Na+/in
Vibrio alginolyticus
-
deamino-NAD+ + ubiquinol + n Na+/out
-
-
?
7.2.1.1
deamino-NADH + H+ + ubiquinone + n Na+/in
Vibrio alginolyticus NCIMB 11038
-
deamino-NAD+ + ubiquinol + n Na+/out
-
-
?
7.2.1.1
NADH + H+ + ubiquinone + n Na+/in
Vibrio alginolyticus
-
NAD+ + ubiquinol + n Na+/out
-
-
?
7.2.1.1
NADH + H+ + ubiquinone + n Na+/in
Vibrio alginolyticus NCIMB 11038
-
NAD+ + ubiquinol + n Na+/out
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
7.2.1.1
Vibrio alginolyticus
-
-
-
7.2.1.1
Vibrio alginolyticus NCIMB 11038
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
7.2.1.1
partially purified by DEAE Sepharose column chromatography and preparative gel electrophoresis
Vibrio alginolyticus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7.2.1.1
deamino-NADH + H+ + ubiquinone + n Na+/in
-
711991
Vibrio alginolyticus
deamino-NAD+ + ubiquinol + n Na+/out
-
-
-
?
7.2.1.1
deamino-NADH + H+ + ubiquinone + n Na+/in
-
711991
Vibrio alginolyticus NCIMB 11038
deamino-NAD+ + ubiquinol + n Na+/out
-
-
-
?
7.2.1.1
NADH + H+ + ubiquinone + n Na+/in
-
711991
Vibrio alginolyticus
NAD+ + ubiquinol + n Na+/out
-
-
-
?
7.2.1.1
NADH + H+ + ubiquinone + n Na+/in
-
711991
Vibrio alginolyticus NCIMB 11038
NAD+ + ubiquinol + n Na+/out
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
7.2.1.1
heterotetramer
1 * 48622 + 1 * 46809 + 1 * 27672 + 1 * 22602, subunits NqrA, NqrB, NqrC and NqrD, calculated from amino acid sequence
Vibrio alginolyticus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
7.2.1.1
FAD
-
Vibrio alginolyticus
7.2.1.1
FMN
-
Vibrio alginolyticus
7.2.1.1
NADH
-
Vibrio alginolyticus
7.2.1.1
[2Fe-2S]-center
-
Vibrio alginolyticus
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
7.2.1.1
Vibrio alginolyticus
subunit NqrC, calculated from amino acid sequence
-
4.9
7.2.1.1
Vibrio alginolyticus
subunit NqrA, calculated from amino acid sequence
-
5.6
7.2.1.1
Vibrio alginolyticus
subunit NqrB, calculated from amino acid sequence
-
9.1
7.2.1.1
Vibrio alginolyticus
subunit NqrD, calculated from amino acid sequence
-
9.3
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
7.2.1.1
FAD
-
Vibrio alginolyticus
7.2.1.1
FMN
-
Vibrio alginolyticus
7.2.1.1
NADH
-
Vibrio alginolyticus
7.2.1.1
[2Fe-2S]-center
-
Vibrio alginolyticus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
7.2.1.1
2-n-heptyl-4-hydroxyquinoline N-oxide
-
Vibrio alginolyticus
7.2.1.1
Ag+
-
Vibrio alginolyticus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
7.2.1.1
membrane
-
Vibrio alginolyticus
16020
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
7.2.1.1
22602
-
1 * 48622 + 1 * 46809 + 1 * 27672 + 1 * 22602, subunits NqrA, NqrB, NqrC and NqrD, calculated from amino acid sequence
Vibrio alginolyticus
7.2.1.1
27672
-
1 * 48622 + 1 * 46809 + 1 * 27672 + 1 * 22602, subunits NqrA, NqrB, NqrC and NqrD, calculated from amino acid sequence
Vibrio alginolyticus
7.2.1.1
46809
-
1 * 48622 + 1 * 46809 + 1 * 27672 + 1 * 22602, subunits NqrA, NqrB, NqrC and NqrD, calculated from amino acid sequence
Vibrio alginolyticus
7.2.1.1
48622
-
1 * 48622 + 1 * 46809 + 1 * 27672 + 1 * 22602, subunits NqrA, NqrB, NqrC and NqrD, calculated from amino acid sequence
Vibrio alginolyticus
7.2.1.1
254000
-
gel filtration
Vibrio alginolyticus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
7.2.1.1
deamino-NADH + H+ + ubiquinone + n Na+/in
Vibrio alginolyticus
-
deamino-NAD+ + ubiquinol + n Na+/out
-
-
?
7.2.1.1
deamino-NADH + H+ + ubiquinone + n Na+/in
Vibrio alginolyticus NCIMB 11038
-
deamino-NAD+ + ubiquinol + n Na+/out
-
-
?
7.2.1.1
NADH + H+ + ubiquinone + n Na+/in
Vibrio alginolyticus
-
NAD+ + ubiquinol + n Na+/out
-
-
?
7.2.1.1
NADH + H+ + ubiquinone + n Na+/in
Vibrio alginolyticus NCIMB 11038
-
NAD+ + ubiquinol + n Na+/out
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
7.2.1.1
partially purified by DEAE Sepharose column chromatography and preparative gel electrophoresis
Vibrio alginolyticus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7.2.1.1
deamino-NADH + H+ + ubiquinone + n Na+/in
-
711991
Vibrio alginolyticus
deamino-NAD+ + ubiquinol + n Na+/out
-
-
-
?
7.2.1.1
deamino-NADH + H+ + ubiquinone + n Na+/in
-
711991
Vibrio alginolyticus NCIMB 11038
deamino-NAD+ + ubiquinol + n Na+/out
-
-
-
?
7.2.1.1
NADH + H+ + ubiquinone + n Na+/in
-
711991
Vibrio alginolyticus
NAD+ + ubiquinol + n Na+/out
-
-
-
?
7.2.1.1
NADH + H+ + ubiquinone + n Na+/in
-
711991
Vibrio alginolyticus NCIMB 11038
NAD+ + ubiquinol + n Na+/out
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
7.2.1.1
heterotetramer
1 * 48622 + 1 * 46809 + 1 * 27672 + 1 * 22602, subunits NqrA, NqrB, NqrC and NqrD, calculated from amino acid sequence
Vibrio alginolyticus
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
7.2.1.1
Vibrio alginolyticus
subunit NqrC, calculated from amino acid sequence
-
4.9
7.2.1.1
Vibrio alginolyticus
subunit NqrA, calculated from amino acid sequence
-
5.6
7.2.1.1
Vibrio alginolyticus
subunit NqrB, calculated from amino acid sequence
-
9.1
7.2.1.1
Vibrio alginolyticus
subunit NqrD, calculated from amino acid sequence
-
9.3