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Literature summary extracted from

  • Cai, Y.; Trodler, P.; Jiang, S.; Zhang, W.; Wu, Y.; Lu, Y.; Yang, S.; Jiang, W.
    Isolation and molecular characterization of a novel D-hydantoinase from Jannaschia sp. CCS1 (2009), FEBS J., 276, 3575-3588.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.2.2 expression in Escherichia coli, His-tag Jannaschia sp. CCS1

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.2.2 docking of the productive transition states of hydantoin, DL-p-hydroxyphenylhydantoin, 1-p-hydroxyphenylhydantoin and dihydrouracil into the active site to simulate the mode of substrate binding. The substrate binding pocket accommodates substrates with small side chains better than those with large ones. The bulky side chains of Phe63, Leu92, Phe150 and Tyr153 are in close contact with the exocyclic substituent of D-p-hydroxyphenylhydantoin Jannaschia sp. CCS1

Protein Variants

EC Number Protein Variants Comment Organism
3.5.2.2 F150C about 5% of wild-type activity Jannaschia sp. CCS1
3.5.2.2 F150D about 5% of wild-type activity Jannaschia sp. CCS1
3.5.2.2 F150E about 5% of wild-type activity Jannaschia sp. CCS1
3.5.2.2 F150G about 8% of wild-type activity Jannaschia sp. CCS1
3.5.2.2 F150H about 5% of wild-type activity Jannaschia sp. CCS1
3.5.2.2 F150I about 10% of wild-type activity Jannaschia sp. CCS1
3.5.2.2 F150K about 5% of wild-type activity Jannaschia sp. CCS1
3.5.2.2 F150L about 10% of wild-type activity Jannaschia sp. CCS1
3.5.2.2 F150M about 15% of wild-type activity Jannaschia sp. CCS1
3.5.2.2 F150N about 5% of wild-type activity Jannaschia sp. CCS1
3.5.2.2 F150P about 5% of wild-type activity Jannaschia sp. CCS1
3.5.2.2 F150Q about 5% of wild-type activity Jannaschia sp. CCS1
3.5.2.2 F150R about 5% of wild-type activity Jannaschia sp. CCS1
3.5.2.2 F150S about 5% of wild-type activity Jannaschia sp. CCS1
3.5.2.2 F150T about 5% of wild-type activity Jannaschia sp. CCS1
3.5.2.2 F150V about 5% of wild-type activity Jannaschia sp. CCS1
3.5.2.2 F150W about 5% of wild-type activity Jannaschia sp. CCS1
3.5.2.2 F150Y about 18% of wild-type activity Jannaschia sp. CCS1
3.5.2.2 F63C about 75% of wild-type activity Jannaschia sp. CCS1
3.5.2.2 F63D about 15% of wild-type activity Jannaschia sp. CCS1
3.5.2.2 F63E about 10% of wild-type activity Jannaschia sp. CCS1
3.5.2.2 F63G about 50% of wild-type activity Jannaschia sp. CCS1
3.5.2.2 F63H about 115% of wild-type activity Jannaschia sp. CCS1
3.5.2.2 F63I about 80% of wild-type activity Jannaschia sp. CCS1
3.5.2.2 F63K about 35% of wild-type activity Jannaschia sp. CCS1
3.5.2.2 F63L about 105% of wild-type activity Jannaschia sp. CCS1
3.5.2.2 F63M about 95% of wild-type activity Jannaschia sp. CCS1
3.5.2.2 F63N about 110% of wild-type activity Jannaschia sp. CCS1
3.5.2.2 F63P about 10% of wild-type activity Jannaschia sp. CCS1
3.5.2.2 F63Q about 25% of wild-type activity Jannaschia sp. CCS1
3.5.2.2 F63R about 30% of wild-type activity Jannaschia sp. CCS1
3.5.2.2 F63S about 75% of wild-type activity Jannaschia sp. CCS1
3.5.2.2 F63T about 70% of wild-type activity Jannaschia sp. CCS1
3.5.2.2 F63V about 70% of wild-type activity Jannaschia sp. CCS1
3.5.2.2 F63W about 95% of wild-type activity Jannaschia sp. CCS1
3.5.2.2 F63Y about 125% of wild-type activity Jannaschia sp. CCS1
3.5.2.2 L157A 33.6% of wild-type activity Jannaschia sp. CCS1
3.5.2.2 L157D 11% of wild-type activity Jannaschia sp. CCS1
3.5.2.2 L157I 94.0% of wild-type activity Jannaschia sp. CCS1
3.5.2.2 L157V 98.3% of wild-type activity Jannaschia sp. CCS1
3.5.2.2 L92C about 25% of wild-type activity Jannaschia sp. CCS1
3.5.2.2 L92D about 10% of wild-type activity Jannaschia sp. CCS1
3.5.2.2 L92E about 10% of wild-type activity Jannaschia sp. CCS1
3.5.2.2 L92F about 95% of wild-type activity Jannaschia sp. CCS1
3.5.2.2 L92G about 10% of wild-type activity Jannaschia sp. CCS1
3.5.2.2 L92H about 15% of wild-type activity Jannaschia sp. CCS1
3.5.2.2 L92I about 120% of wild-type activity Jannaschia sp. CCS1
3.5.2.2 L92K about 20% of wild-type activity Jannaschia sp. CCS1
3.5.2.2 L92M about 115% of wild-type activity Jannaschia sp. CCS1
3.5.2.2 L92N about 10% of wild-type activity Jannaschia sp. CCS1
3.5.2.2 L92P about 5% of wild-type activity Jannaschia sp. CCS1
3.5.2.2 L92Q about 15% of wild-type activity Jannaschia sp. CCS1
3.5.2.2 L92R about 5% of wild-type activity Jannaschia sp. CCS1
3.5.2.2 L92S about 10% of wild-type activity Jannaschia sp. CCS1
3.5.2.2 L92T about 35% of wild-type activity Jannaschia sp. CCS1
3.5.2.2 L92V about 70% of wild-type activity Jannaschia sp. CCS1
3.5.2.2 L92W about 10% of wild-type activity Jannaschia sp. CCS1
3.5.2.2 L92Y about 5% of wild-type activity Jannaschia sp. CCS1

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.2 18
-
 DL-p-hydroxyphenylhydantoin pH 8.0, 40°C Jannaschia sp. CCS1

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.2.2 56000
-
 4 * 56000, His-tagged recombinant protein Jannaschia sp. CCS1
3.5.2.2 253000
-
 gel filtration Jannaschia sp. CCS1

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.2 Jannaschia sp. CCS1 Q28SA7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.2 recombinant protein Jannaschia sp. CCS1

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.2.2 8.2
-
 40°C, pH 8.0 Jannaschia sp. CCS1

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.2 5,6-dihydrouracil + H2O
-
 Jannaschia sp. CCS1 3-ureidopropionate
-
 ?
3.5.2.2 dimethylhydantoin + H2O 1.4% of the activity with dihydrouracil Jannaschia sp. CCS1 ?
-
 ?
3.5.2.2 DL-5-phenylhydantoin + H2O 45% of the activity with dihydrouracil Jannaschia sp. CCS1 N-carbamoyl-D-phenylglycine
-
 ?
3.5.2.2 DL-p-hydroxyphenylhydantoin + H2O 7.2% of the activity with dihydrouracil Jannaschia sp. CCS1 N-carbamoyl-D-p-hydroxyphenylglycine
-
 ?
3.5.2.2 hydantoin + H2O 18.7% of the activity with dihydrouracil Jannaschia sp. CCS1 hydantoic acid
-
 ?
3.5.2.2 additional information no substrate: diphenylhydantoin, 5-(hydroxymethyl)uracil, benzylhydantoin, isopropylhydantoin Jannaschia sp. CCS1 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.2.2 tetramer 4 * 56000, His-tagged recombinant protein Jannaschia sp. CCS1

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.2.2 50
-
-
 Jannaschia sp. CCS1

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.2.2 4.6
-
 DL-p-hydroxyphenylhydantoin pH 8.0, 40°C Jannaschia sp. CCS1

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.2.2 7.6
-
-
 Jannaschia sp. CCS1

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5.2.2 0.25
-
 DL-p-hydroxyphenylhydantoin pH 8.0, 40°C Jannaschia sp. CCS1