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Literature summary extracted from

  • Omura, H.; Wieser, M.; Nagasawa, T.
    Pyrrole-2-carboxylate decarboxylase from Bacillus megaterium PYR2910, an organic-acid-requiring enzyme (1998), Eur. J. Biochem., 253, 480-484.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.1.93 acetate if a carboxylic acid, such as acetate, is omitted from the reaction using the pure enzyme, no enzyme activity is found. As soon as a carboxylic acid is added, the decarboxylation starts immediately. The enzyme activity is increased with the number of carbon atoms, rising from formate to butyrate. Above four carbon atoms, the activity decreases. Pimelate, butyrate and propionate are the strongest activators Priestia megaterium
4.1.1.93 Butyrate if a carboxylic acid, such as acetate, is omitted from the reaction using the pure enzyme, no enzyme activity is found. As soon as a carboxylic acid is added, the decarboxylation starts immediately. The enzyme activity is increased with the number of carbon atoms, rising from formate to butyrate. Above four carbon atoms, the activity decreases. Pimelate, butyrate and propionate are the strongest activators Priestia megaterium
4.1.1.93 formate if a carboxylic acid, such as acetate, is omitted from the reaction using the pure enzyme, no enzyme activity is found. As soon as a carboxylic acid is added, the decarboxylation starts immediately. The enzyme activity is increased with the number of carbon atoms, rising from formate to butyrate. Above four carbon atoms, the activity decreases. Pimelate, butyrate and propionate are the strongest activators Priestia megaterium
4.1.1.93 pimelate if a carboxylic acid, such as acetate, is omitted from the reaction using the pure enzyme, no enzyme activity is found. As soon as a carboxylic acid is added, the decarboxylation starts immediately. The enzyme activity is increased with the number of carbon atoms, rising from formate to butyrate. Above four carbon atoms, the activity decreases. Pimelate, butyrate and propionate are the strongest activators Priestia megaterium
4.1.1.93 propionate if a carboxylic acid, such as acetate, is omitted from the reaction using the pure enzyme, no enzyme activity is found. As soon as an carboxylic acid is added, the decarboxylation starts immediately. The enzyme activity is increased with the number of carbon atoms, rising from formate to butyrate. Above four carbon atoms, the activity decreases. Pimelate, butyrate and propionate are the strongest activators Priestia megaterium

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.93 3-methyl-2-benzothiazolinonehydrazone 1 mM, 26% inhibition Priestia megaterium
4.1.1.93 5,5'-dithiobis(2-nitrobenzoic acid) 1 mM, 40% inhibition Priestia megaterium
4.1.1.93 AgNO3 1 mM, complete inhibition Priestia megaterium
4.1.1.93 CuCl2 1 mM, 97% inhibition Priestia megaterium
4.1.1.93 cysteamine 1 mM, 25% inhibition Priestia megaterium
4.1.1.93 HgCl2 1 mM, complete inhibition Priestia megaterium
4.1.1.93 KCN 1 mM, 68% inhibition Priestia megaterium
4.1.1.93 NEM 1 mM, 15% inhibition Priestia megaterium
4.1.1.93 p-chloromercuribenzoate 1 mM, 75% inhibition Priestia megaterium
4.1.1.93 phenylhydrazine 1 mM, 84% inhibition Priestia megaterium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.93 52000
-
 2 * 52000, SDS-PAGE Priestia megaterium
4.1.1.93 98000
-
 gel filtration Priestia megaterium

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.93 Priestia megaterium
-
-
-
4.1.1.93 Priestia megaterium PYR2910
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.93
-
 Priestia megaterium

Storage Stability

EC Number Storage Stability Organism
4.1.1.93 -20°C, with dithiothreitol and glycerol, stable for 6 months Priestia megaterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.93 pyrrole-2-carboxylate the enzyme catalyses both the carboxylation and decarboxylation reactions. However, while bicarbonate is the preferred substrate for the carboxylation reaction, decarboxylation produces carbon dioxide Priestia megaterium pyrrole + CO2
-
 ?
4.1.1.93 pyrrole-2-carboxylate the enzyme catalyses both the carboxylation and decarboxylation reactions. However, while bicarbonate is the preferred substrate for the carboxylation reaction, decarboxylation produces carbon dioxide Priestia megaterium PYR2910 pyrrole + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.93 dimer 2 * 52000, SDS-PAGE Priestia megaterium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.93 30
-
 assay, decarboxylation Priestia megaterium
4.1.1.93 45
-
-
 Priestia megaterium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.1.93 50
-
 pH 7.0, stable below Priestia megaterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.93 6.5
-
-
 Priestia megaterium
4.1.1.93 7
-
 assay, decarboxylation Priestia megaterium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.1.93 4 8.5 pH 4.0: about 40% of maximal activity, pH 8.5: about 60% of maximal activity Priestia megaterium

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.1.1.93 6 9 stable Priestia megaterium