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Literature summary extracted from

  • Isobe, K.; Tamauchi, H.; Fuhshuku, K.; Nagasawa, S.; Asano, Y.
    A simple enzymatic method for production of a wide variety of D-amino acids using L-amino acid oxidase from Rhodococcus sp. AIU Z-35-1 (2010), Enzyme Res., 2010, 567210.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
1.4.3.2 additional information development of a simple enzymatic method for production of a wide variety of D-amino acids using L-amino acid oxidase from Rhodococcus sp. AIU Z-35-1, overview Rhodococcus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.3.2 L-lysine + H2O + O2 Rhodococcus sp.
-
 6-amino-2-oxohexanoic acid + NH3 + H2O2
-
 ?
1.4.3.2 L-lysine + H2O + O2 Rhodococcus sp. AIU Z-35-1
-
 6-amino-2-oxohexanoic acid + NH3 + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.2 Rhodococcus sp.
-
-
-
1.4.3.2 Rhodococcus sp. AIU Z-35-1
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.2 L-arginine + H2O + O2
-
 Rhodococcus sp. 2-oxo-5-guanidinopentanoic acid + NH3 + H2O2
-
 ?
1.4.3.2 L-arginine + H2O + O2
-
 Rhodococcus sp. AIU Z-35-1 2-oxo-5-guanidinopentanoic acid + NH3 + H2O2
-
 ?
1.4.3.2 L-citrulline + H2O + O2
-
 Rhodococcus sp. 2-oxo-5-ureidovaleric acid + NH3 + H2O2
-
 ?
1.4.3.2 L-glutamate + H2O + O2
-
 Rhodococcus sp. 2-oxoglutaric acid + NH3 + H2O2
-
 ?
1.4.3.2 L-glutamine + H2O + O2
-
 Rhodococcus sp. 2-oxo-4-carbamoylbutanoic acid + NH3 + H2O2
-
 ?
1.4.3.2 L-glutamine + H2O + O2
-
 Rhodococcus sp. AIU Z-35-1 2-oxo-4-carbamoylbutanoic acid + NH3 + H2O2
-
 ?
1.4.3.2 L-histidine + H2O + O2
-
 Rhodococcus sp. 2-oxo-4-imidazolepropionic acid + NH3 + H2O2
-
 ?
1.4.3.2 L-homoserine + H2O + O2
-
 Rhodococcus sp. 2-oxo-4-hydroxybutyric acid + NH3 + H2O2
-
 ?
1.4.3.2 L-leucine + H2O + O2
-
 Rhodococcus sp. 2-oxo-4-methylvaleric acid + NH3 + H2O2
-
 ?
1.4.3.2 L-lysine + H2O + O2
-
 Rhodococcus sp. 6-amino-2-oxohexanoic acid + NH3 + H2O2
-
 ?
1.4.3.2 L-lysine + H2O + O2
-
 Rhodococcus sp. AIU Z-35-1 6-amino-2-oxohexanoic acid + NH3 + H2O2
-
 ?
1.4.3.2 L-phenylalanine + H2O + O2
-
 Rhodococcus sp. phenylpyruvate + NH3 + H2O2
-
 ?
1.4.3.2 L-phenylalanine + H2O + O2
-
 Rhodococcus sp. AIU Z-35-1 phenylpyruvate + NH3 + H2O2
-
 ?
1.4.3.2 additional information the enzyme from Rhodococcus sp. AIU Z-35-1 shows broad substrate specificity Rhodococcus sp. ?
-
 ?
1.4.3.2 additional information the enzyme from Rhodococcus sp. AIU Z-35-1 shows broad substrate specificity Rhodococcus sp. AIU Z-35-1 ?
-
 ?
1.4.3.2 Nepsilon-acetyl-L-lysine + H2O + O2
-
 Rhodococcus sp. 2-oxo-6-acetylaminohexanoic acid + NH3 + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.4.3.2 L-AAO
-
 Rhodococcus sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.3.2 30
-
 assay at Rhodococcus sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.3.2 30 50 pH 7.0, 60 min, stable up to 30°C, 80% of the enzyme activity remains at 40°C, the enzyme activity is almost completely lost at 50°C Rhodococcus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.2 7
-
 assay at Rhodococcus sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.4.3.2 5.5 8.5 activity assay range Rhodococcus sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.4.3.2 5.5 7 40°C, 60 min, loss of about 20-30% activity Rhodococcus sp.
1.4.3.2 7 8.5 40°C, 60 min, highly stable Rhodococcus sp.