Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Shuster Ben-Yosef, V.; Sendovski, M.; Fishman, A.
    Directed evolution of tyrosinase for enhanced monophenolase/diphenolase activity ratio (2010), Enzyme Microb. Technol., 47, 372-376.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.18.1 expressed in Escherichia coli BL21(DE3) cells Priestia megaterium

Protein Variants

EC Number Protein Variants Comment Organism
1.14.18.1 R209H the mutant exhibits a 1.7fold increase in monophenolase activity accompanied by a 1.5fold decrease in diphenolase activity, resulting in a 2.6fold improvement in the monophenolase/diphenolase activity ratio Priestia megaterium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.18.1 0.038
-
 L-tyrosine wild type enzyme, in 50 mM Tris-HCl buffer pH 7.0, 0.01 mM CuSO4, 28°C Priestia megaterium
1.14.18.1 0.4
-
 L-Dopa wild type enzyme, in 50 mM Tris-HCl buffer pH 7.0, 0.01 mM CuSO4, 28°C Priestia megaterium
1.14.18.1 0.62
-
 L-Dopa mutant enzyme R209H, in 50 mM Tris-HCl buffer pH 7.0, 0.01 mM CuSO4, 28°C Priestia megaterium
1.14.18.1 1.2
-
 D-Dopa wild type enzyme, in 50 mM Tris-HCl buffer pH 7.0, 0.01 mM CuSO4, 28°C Priestia megaterium
1.14.18.1 1.3
-
 L-tyrosine mutant enzyme R209H, in 50 mM Tris-HCl buffer pH 7.0, 0.01 mM CuSO4, 28°C Priestia megaterium
1.14.18.1 3.8
-
 D-Dopa mutant enzyme R209H, in 50 mM Tris-HCl buffer pH 7.0, 0.01 mM CuSO4, 28°C Priestia megaterium

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.1 Priestia megaterium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.18.1 HisTrap column chromatography, gel filtration Priestia megaterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.1 D-DOPA + O2
-
 Priestia megaterium D-dopaquinone + H2O
-
 ?
1.14.18.1 L-Dopa + O2
-
 Priestia megaterium L-dopaquinone + H2O
-
 ?
1.14.18.1 L-tyrosine + O2
-
 Priestia megaterium L-DOPA + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.18.1 tyrosinase
-
 Priestia megaterium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.18.1 1.29
-
 L-tyrosine wild type enzyme, in 50 mM Tris-HCl buffer pH 7.0, 0.01 mM CuSO4, 28°C Priestia megaterium
1.14.18.1 2.24
-
 L-tyrosine mutant enzyme R209H, in 50 mM Tris-HCl buffer pH 7.0, 0.01 mM CuSO4, 28°C Priestia megaterium
1.14.18.1 6.35
-
 D-Dopa wild type enzyme, in 50 mM Tris-HCl buffer pH 7.0, 0.01 mM CuSO4, 28°C Priestia megaterium
1.14.18.1 6.88
-
 D-Dopa mutant enzyme R209H, in 50 mM Tris-HCl buffer pH 7.0, 0.01 mM CuSO4, 28°C Priestia megaterium
1.14.18.1 7.18
-
 L-Dopa mutant enzyme R209H, in 50 mM Tris-HCl buffer pH 7.0, 0.01 mM CuSO4, 28°C Priestia megaterium
1.14.18.1 10.59
-
 L-Dopa wild type enzyme, in 50 mM Tris-HCl buffer pH 7.0, 0.01 mM CuSO4, 28°C Priestia megaterium

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.14.18.1 1.72
-
 L-tyrosine mutant enzyme R209H, in 50 mM Tris-HCl buffer pH 7.0, 0.01 mM CuSO4, 28°C Priestia megaterium
1.14.18.1 1.81
-
 D-Dopa mutant enzyme R209H, in 50 mM Tris-HCl buffer pH 7.0, 0.01 mM CuSO4, 28°C Priestia megaterium
1.14.18.1 5.29
-
 D-Dopa wild type enzyme, in 50 mM Tris-HCl buffer pH 7.0, 0.01 mM CuSO4, 28°C Priestia megaterium
1.14.18.1 11.58
-
 L-Dopa mutant enzyme R209H, in 50 mM Tris-HCl buffer pH 7.0, 0.01 mM CuSO4, 28°C Priestia megaterium
1.14.18.1 26.47
-
 L-Dopa wild type enzyme, in 50 mM Tris-HCl buffer pH 7.0, 0.01 mM CuSO4, 28°C Priestia megaterium
1.14.18.1 34.05
-
 L-tyrosine wild type enzyme, in 50 mM Tris-HCl buffer pH 7.0, 0.01 mM CuSO4, 28°C Priestia megaterium