EC Number | Cloned (Comment) | Organism |
---|---|---|
1.17.1.10 | expressed in Escherichia coli BL21(DE3) cells | Burkholderia stabilis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.17.1.10 | Q223E | the mutated enzyme shows an 18fold increment in catalytic efficiency with NAD+ with a concomitant 185fold reduction in the efficiency with NADP+, drastically shifting the cofactor preference from NADP+ to NAD+ | Burkholderia stabilis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.17.1.10 | 5,5'-dithio-bis(2-nitrobenzoic acid) | complete inhibition at 1 mM | Burkholderia stabilis | |
1.17.1.10 | AgNO3 | complete inhibition at 1 mM | Burkholderia stabilis | |
1.17.1.10 | CdCl2 | slight inhibition at 1 mM | Burkholderia stabilis | |
1.17.1.10 | CoCl2 | slight inhibition at 1 mM | Burkholderia stabilis | |
1.17.1.10 | CuSO4 | slight inhibition at 1 mM | Burkholderia stabilis | |
1.17.1.10 | FeCl2 | complete inhibition at 1 mM | Burkholderia stabilis | |
1.17.1.10 | FeSO4 | slight inhibition at 1 mM | Burkholderia stabilis | |
1.17.1.10 | HgCl2 | complete inhibition at 1 mM | Burkholderia stabilis | |
1.17.1.10 | hydroxylamine | slight inhibition at 10 mM | Burkholderia stabilis | |
1.17.1.10 | additional information | ZnCl2 (1 mM), 10 mM of Mg2SO4, dithiothreitol, mercaptoethanol and phenylmethylsulfonyl fluoride do not affect enzyme activity | Burkholderia stabilis | |
1.17.1.10 | NaNO3 | slight inhibition at 1 mM | Burkholderia stabilis | |
1.17.1.10 | Sodium azide | complete inhibition at 1 mM | Burkholderia stabilis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.17.1.10 | 0.06 | - |
NAD+ | mutant enzyme Q223E, in 1 M potassium phosphate buffer (pH 7.0), at 30°C | Burkholderia stabilis | |
1.17.1.10 | 0.16 | - |
NADP+ | wild type enzyme, in 1 M potassium phosphate buffer (pH 7.0), at 30°C | Burkholderia stabilis | |
1.17.1.10 | 0.83 | - |
NADP+ | mutant enzyme Q223E, in 1 M potassium phosphate buffer (pH 7.0), at 30°C | Burkholderia stabilis | |
1.17.1.10 | 1.43 | - |
NAD+ | wild type enzyme, in 1 M potassium phosphate buffer (pH 7.0), at 30°C | Burkholderia stabilis | |
1.17.1.10 | 55.5 | - |
formate | wild type enzyme, in 1 M potassium phosphate buffer (pH 7.0), at 30°C | Burkholderia stabilis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.17.1.10 | 42000 | - |
8 * 42000, SDS-PAGE | Burkholderia stabilis |
1.17.1.10 | 340000 | - |
gel filtration | Burkholderia stabilis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.17.1.10 | Burkholderia stabilis | B5A8W5 | - |
- |
1.17.1.10 | Burkholderia stabilis 15516 | B5A8W5 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.17.1.10 | DEAE-Toyopearl column chromatography and butyl-Toyopearl 650M column chromatography | Burkholderia stabilis |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.17.1.10 | 1.4 | - |
crude enzyme, in 1 M potassium phosphate buffer (pH 7.0), at 30°C | Burkholderia stabilis |
1.17.1.10 | 5.3 | - |
after 3.8fold purification, in 1 M potassium phosphate buffer (pH 7.0), at 30°C | Burkholderia stabilis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.17.1.10 | formate + NAD+ | the activity to NAD+ is 38% of that to NADP+ | Burkholderia stabilis | CO2 + NADH + H+ | - |
? | |
1.17.1.10 | formate + NAD+ | the activity to NAD+ is 38% of that to NADP+ | Burkholderia stabilis 15516 | CO2 + NADH + H+ | - |
? | |
1.17.1.10 | formate + NADP+ | the enzyme acts only on formate as the substrate | Burkholderia stabilis | CO2 + NADPH + H+ | - |
? | |
1.17.1.10 | formate + NADP+ | the enzyme acts only on formate as the substrate | Burkholderia stabilis 15516 | CO2 + NADPH + H+ | - |
? | |
1.17.1.10 | additional information | methanol, formaldehyde, acetic acid, ethanol, propanol and glycerol are inert. When artificial electron acceptors are used, ferricyanide, nitro blue tetrazolium, phenazine methosulfate, riboflavin, ATP and FAD are not reduced in the presence of formate | Burkholderia stabilis | ? | - |
? | |
1.17.1.10 | additional information | methanol, formaldehyde, acetic acid, ethanol, propanol and glycerol are inert. When artificial electron acceptors are used, ferricyanide, nitro blue tetrazolium, phenazine methosulfate, riboflavin, ATP and FAD are not reduced in the presence of formate | Burkholderia stabilis 15516 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.17.1.10 | homooctamer | 8 * 42000, SDS-PAGE | Burkholderia stabilis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.17.1.10 | FDH | - |
Burkholderia stabilis |
1.17.1.10 | NADP+-dependent formate dehydrogenase | - |
Burkholderia stabilis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.17.1.10 | 60 | - |
- |
Burkholderia stabilis |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.17.1.10 | 55 | 60 | no loss of activity is observed at 55°C for 10 h, but about half of the enzyme activity is lost after incubation for 36 h. The enzyme can maintain a 50% activity for at least 5 h when it is incubated at 60°C | Burkholderia stabilis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.17.1.10 | 0.13 | - |
NADP+ | mutant enzyme Q223E, in 1 M potassium phosphate buffer (pH 7.0), at 30°C | Burkholderia stabilis | |
1.17.1.10 | 1.25 | - |
NAD+ | mutant enzyme Q223E, in 1 M potassium phosphate buffer (pH 7.0), at 30°C | Burkholderia stabilis | |
1.17.1.10 | 1.66 | - |
NAD+ | wild type enzyme, in 1 M potassium phosphate buffer (pH 7.0), at 30°C | Burkholderia stabilis | |
1.17.1.10 | 4.75 | - |
NADP+ | wild type enzyme, in 1 M potassium phosphate buffer (pH 7.0), at 30°C | Burkholderia stabilis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.17.1.10 | 6 | 7.5 | - |
Burkholderia stabilis |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.17.1.10 | 5 | 12 | more than 94% of the initial activity is retained at pH 5.0-12.0 after incubation for 30 min at 30°C | Burkholderia stabilis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.17.1.10 | NADP+ | the enzyme accepts NADP+ as the natural coenzyme with a much higher specificity over NAD+, the activity to NAD+ is 38% of that to NADP+ | Burkholderia stabilis |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.17.1.10 | 0.16 | - |
NADP+ | mutant enzyme Q223E, in 1 M potassium phosphate buffer (pH 7.0), at 30°C | Burkholderia stabilis | |
1.17.1.10 | 1.16 | - |
NAD+ | wild type enzyme, in 1 M potassium phosphate buffer (pH 7.0), at 30°C | Burkholderia stabilis | |
1.17.1.10 | 20 | - |
NAD+ | mutant enzyme Q223E, in 1 M potassium phosphate buffer (pH 7.0), at 30°C | Burkholderia stabilis | |
1.17.1.10 | 30 | - |
NADP+ | wild type enzyme, in 1 M potassium phosphate buffer (pH 7.0), at 30°C | Burkholderia stabilis |