BRENDA - Enzyme Database show

Nicotinamidase modulation of NAD+ biosynthesis and nicotinamide levels separately affect reproductive development and cell survival in C. elegans

Vrablik, T.L.; Huang, L.; Lange, S.E.; Hanna-Rose, W.; Development 136, 3637-3646 (2009)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.5.1.19
isolation of a pnc-1a promoter consisting of exon 1a and the intervening region between exon 1a and the adjacent gene, expression as GFP-fusion protein. Expression of His-tagged PNC proteins in Escherichia coli strain BL21(DE3)
Caenorhabditis elegans
Engineering
EC Number
Amino acid exchange
Commentary
Organism
3.5.1.19
additional information
mutations in PNC-1 cause developmental and functional defects in the reproductive system. The development of the gonad is delayed, four uterine cells die by necrosis and the mutant animals are egg-laying defective. The temporal delay in gonad development results from depletion of the salvage pathway product NAD+, whereas the uv1 cell necrosis and egg-laying defects result from accumulation of the substrate nicotinamide, phenotype, overview. The bacterial food source partially compensates for pnc-1 reproductive defects. Although the secreted isoform is sufficient to rescue all phenotypes, the concentrations of NAD+ and nicotinamide in the cytoplasm and/or cellular organelles presumably elicit phenotypes
Caenorhabditis elegans
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.5.1.19
extracellular
secreted isoform of PNC-1
Caenorhabditis elegans
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.1.19
Mg2+
required
Caenorhabditis elegans
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.1.19
nicotinamide + H2O
Caenorhabditis elegans
-
nicotinate + NH3
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.1.19
Caenorhabditis elegans
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.19
nicotinamide + H2O
-
711827
Caenorhabditis elegans
nicotinate + NH3
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.1.19
30
-
assay at
Caenorhabditis elegans
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.1.19
7.5
-
assay at
Caenorhabditis elegans
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.1.19
isolation of a pnc-1a promoter consisting of exon 1a and the intervening region between exon 1a and the adjacent gene, expression as GFP-fusion protein. Expression of His-tagged PNC proteins in Escherichia coli strain BL21(DE3)
Caenorhabditis elegans
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
3.5.1.19
additional information
mutations in PNC-1 cause developmental and functional defects in the reproductive system. The development of the gonad is delayed, four uterine cells die by necrosis and the mutant animals are egg-laying defective. The temporal delay in gonad development results from depletion of the salvage pathway product NAD+, whereas the uv1 cell necrosis and egg-laying defects result from accumulation of the substrate nicotinamide, phenotype, overview. The bacterial food source partially compensates for pnc-1 reproductive defects. Although the secreted isoform is sufficient to rescue all phenotypes, the concentrations of NAD+ and nicotinamide in the cytoplasm and/or cellular organelles presumably elicit phenotypes
Caenorhabditis elegans
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.5.1.19
extracellular
secreted isoform of PNC-1
Caenorhabditis elegans
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.1.19
Mg2+
required
Caenorhabditis elegans
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.1.19
nicotinamide + H2O
Caenorhabditis elegans
-
nicotinate + NH3
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.19
nicotinamide + H2O
-
711827
Caenorhabditis elegans
nicotinate + NH3
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.1.19
30
-
assay at
Caenorhabditis elegans
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.1.19
7.5
-
assay at
Caenorhabditis elegans
General Information
EC Number
General Information
Commentary
Organism
3.5.1.19
metabolism
nicotinamide phosphoribosyltransferase, Nampt, the equivalent enzyme in nicotinamide recycling to NAD+ in vertebrates, can functionally substitute for PNC-1
Caenorhabditis elegans
3.5.1.19
additional information
a correlation between the limited detected expression patterns of the PNC-1a and b promoters and the function of PNC-1 in development is not obvious
Caenorhabditis elegans
General Information (protein specific)
EC Number
General Information
Commentary
Organism
3.5.1.19
metabolism
nicotinamide phosphoribosyltransferase, Nampt, the equivalent enzyme in nicotinamide recycling to NAD+ in vertebrates, can functionally substitute for PNC-1
Caenorhabditis elegans
3.5.1.19
additional information
a correlation between the limited detected expression patterns of the PNC-1a and b promoters and the function of PNC-1 in development is not obvious
Caenorhabditis elegans