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Literature summary extracted from

  • Lee, A.C.; Lee, K.T.; Pan, L.Y.
    Purification and kinetic characteristics of strombine dehydrogenase from the foot muscle of the hard clam (Meretrix lusoria) (2010), Comp. Biochem. Physiol. B, 158, 38-45.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.22 acetate
-
 Meretrix lusoria
1.5.1.22 D-lactate
-
 Meretrix lusoria
1.5.1.22 iminodiacetate
-
 Meretrix lusoria
1.5.1.22 L-lactate
-
 Meretrix lusoria
1.5.1.22 oxaloacetate
-
 Meretrix lusoria
1.5.1.22 succinate
-
 Meretrix lusoria

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.22 1.39
-
 pyruvate pH 7.5, 25°C Meretrix lusoria
1.5.1.22 34.2
-
 glycine pH 7.5, 25°C Meretrix lusoria
1.5.1.22 39.2
-
 L-Ala pH 7.5, 25°C Meretrix lusoria
1.5.1.22 45.9
-
 L-2-aminobutyrate pH 7.5, 25°C Meretrix lusoria
1.5.1.22 163
-
 L-Ser pH 7.5, 25°C Meretrix lusoria
1.5.1.22 196
-
 beta-Ala pH 7.5, 25°C Meretrix lusoria
1.5.1.22 303
-
 L-Thr pH 7.5, 25°C Meretrix lusoria

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.5.1.22 Ca2+ 10% of enzymatic activity is inhibited at 10 mM Meretrix lusoria
1.5.1.22 Cu2+ 50% of enzymatic activity is inhibited at 1 mM Meretrix lusoria
1.5.1.22 Fe3+ inhibits enzyme at 0.2 mM Meretrix lusoria
1.5.1.22 Mg2+ 20% of enzymatic activity is inhibited at 10 mM Meretrix lusoria
1.5.1.22 Mn2+ 13% of enzymatic activity is inhibited at 1 mM Meretrix lusoria
1.5.1.22 Zn2+ inhibits enzyme at 0.6 mM Meretrix lusoria

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.22 46000
-
 SDS-PAGE Meretrix lusoria

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.22 Meretrix lusoria
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.22 purified over 470fold using ammonium sulfate fractionation, sephacryl S-100 chromatography, hydroxyapatite adsorption, DEAE-sepharose chromatography, Blue-sepharose chromatography Meretrix lusoria

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.22 muscle
-
 Meretrix lusoria
-

Storage Stability

EC Number Storage Stability Organism
1.5.1.22 4°C, glycerol, 2 weeks, 100% activity Meretrix lusoria

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.22 beta-Ala + pyruvate + NADH
-
 Meretrix lusoria ?
-
 ?
1.5.1.22 glycine + pyruvate + NADH Gly and Ala are preferred substrates Meretrix lusoria D-strombine + NAD+ + H2O
-
 ?
1.5.1.22 L-2-Aminobutyrate + pyruvate + NADH
-
 Meretrix lusoria ?
-
 ?
1.5.1.22 L-Ala + pyruvate + NADH Gly and Ala are preferred substrates Meretrix lusoria ?
-
 ?
1.5.1.22 L-Ser + pyruvate + NADH
-
 Meretrix lusoria ?
-
 ?
1.5.1.22 L-Thr + pyruvate + NADH
-
 Meretrix lusoria ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.22 monomer gel filtration, SDS-PAGE analysis, 1 * 46000 Meretrix lusoria

Synonyms

EC Number Synonyms Comment Organism
1.5.1.22 SDH
-
 Meretrix lusoria
1.5.1.22 strombine dehydrogenase
-
 Meretrix lusoria

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.1.22 25
-
 assay at Meretrix lusoria

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.5.1.22 45 46 temperature optimum Meretrix lusoria

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.5.1.22 56
-
 enzyme is heat labile, pretreatment at 56°C for 20 sec only retains 20% of its original activity Meretrix lusoria

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.22 7.5
-
 assay at Meretrix lusoria

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.5.1.22 7.4 7.6 pH optimum Meretrix lusoria

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.5.1.22 0.63
-
 iminodiacetate pH 7.5, 25°C Meretrix lusoria
1.5.1.22 1.35
-
 iminodiacetate pH 6.5, 25°C Meretrix lusoria
1.5.1.22 3.69
-
 acetate pH 7.5, 25°C Meretrix lusoria
1.5.1.22 3.98
-
 succinate pH 6.5, 25°C Meretrix lusoria
1.5.1.22 5.57
-
 acetate pH 6.5, 25°C Meretrix lusoria
1.5.1.22 6.93
-
 oxaloacetate pH 7.5, 25°C Meretrix lusoria
1.5.1.22 14.3
-
 oxaloacetate pH 6.5, 25°C Meretrix lusoria
1.5.1.22 15.4
-
 succinate pH 7.5, 25°C Meretrix lusoria
1.5.1.22 16.4
-
 D-lactate pH 6.5, 25°C Meretrix lusoria
1.5.1.22 17
-
 D-lactate pH 7.5, 25°C Meretrix lusoria
1.5.1.22 21.2
-
 L-lactate pH 6.5, 25°C Meretrix lusoria
1.5.1.22 22.9
-
 L-lactate pH 7.5, 25°C Meretrix lusoria