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Literature summary extracted from

  • Ferry, G.; Hecht, S.; Berger, S.; Moulharat, N.; Coge, F.; Guillaumet, G.; Leclerc, V.; Yous, S.; Delagrange, P.; Boutin, J.A.
    Old and new inhibitors of quinone reductase 2 (2010), Chem. Biol. Interact., 186, 103-109.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.10.5.1 the His-tagged enzyme is expressed in the Sf9 Spodoptera frugiperda ovarian cell line ATCC CRL-1711 Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.5.1 chrysoeriol
-
 Homo sapiens
1.10.5.1 Melatonin QR2 is inhibited in the micromolar range by melatonin, but not when using N-benzyl dihydronicotinamide and coenzyme Q2 are used as substrates Homo sapiens
1.10.5.1 additional information dicoumarol is inactive up to 0.1 mM Homo sapiens
1.10.5.1 resveratrol
-
 Homo sapiens
1.10.5.1 S26695 i.e. N-[2-(7-methylaminosulfonyl-1-naphthyl)ethyl]acetamide Homo sapiens
1.10.5.1 S29434 i.e. N-[2-(2-methoxy-6H-dipyrido[2,3-a:3,2-e]pyrrolizin-11-yl)ethyl]-2-furamide, inhibits QR2 activity with an IC50 in the low nanomolar range from 0.7 to 80 nM, depending on the substrates and co-substrates used Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.5.1 0.0102
-
 2,6-dichlorophenolindophenol using N-benzyl dihydronicotinamide as cosubstrate, at 25°C, in 50 mM Tris-HCl, pH 8.5 Homo sapiens
1.10.5.1 0.0111
-
 menadione using N-benzyl dihydronicotinamide as cosubstrate, at 25°C, in 50 mM Tris-HCl, pH 8.5 Homo sapiens
1.10.5.1 0.0113
-
 N-benzyl dihydronicotinamide using menadione as cosubstrate, at 25°C, in 50 mM Tris-HCl, pH 8.5 Homo sapiens
1.10.5.1 0.0137
-
 coenzyme Q0 using N-benzyl dihydronicotinamide as cosubstrate, at 25°C, in 50 mM Tris-HCl, pH 8.5 Homo sapiens
1.10.5.1 0.0361
-
 N-benzyl dihydronicotinamide using coenzyme Q0 as cosubstrate, at 25°C, in 50 mM Tris-HCl, pH 8.5 Homo sapiens
1.10.5.1 0.0387
-
 coenzyme Q2 using N-benzyl dihydronicotinamide as cosubstrate, at 25°C, in 50 mM Tris-HCl, pH 8.5 Homo sapiens
1.10.5.1 0.0404
-
 N-benzyl dihydronicotinamide using 2,6-dichlorophenolindophenol as cosubstrate, at 25°C, in 50 mM Tris-HCl, pH 8.5 Homo sapiens
1.10.5.1 0.0451
-
 N-benzyl dihydronicotinamide using coenzyme Q1 as cosubstrate, at 25°C, in 50 mM Tris-HCl, pH 8.5 Homo sapiens
1.10.5.1 0.0517
-
 coenzyme Q1 using N-benzyl dihydronicotinamide as cosubstrate, at 25°C, in 50 mM Tris-HCl, pH 8.5 Homo sapiens
1.10.5.1 0.0602
-
 N-benzyl dihydronicotinamide using coenzyme Q2 as cosubstrate, at 25°C, in 50 mM Tris-HCl, pH 8.5 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.10.5.1 cytosol
-
 Homo sapiens 5829
-

Organism

EC Number Organism UniProt Comment Textmining
1.10.5.1 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.5.1 Ni-NTA column chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.5.1 additional information the enzyme does not accept conventional phosphorylated nicotinamides as hydride donors Homo sapiens ?
-
 ?
1.10.5.1 N-benzyldihydronicotinamide + 2,6-dichlorophenolindophenol
-
 Homo sapiens N-benzylnicotinamide + reduced 2,6-dichlorophenolindophenol
-
 ?
1.10.5.1 N-benzyldihydronicotinamide + menadione
-
 Homo sapiens N-benzylnicotinamide + menadiol
-
 ?
1.10.5.1 N-benzyldihydronicotinamide + oxidized coenzyme Q0
-
 Homo sapiens N-benzylnicotinamide + reduced coenzyme Q20
-
 ?
1.10.5.1 N-benzyldihydronicotinamide + oxidized coenzyme Q1
-
 Homo sapiens N-benzylnicointamide + reduced coenzyme Q1
-
 ?
1.10.5.1 N-benzyldihydronicotinamide + oxidized coenzyme Q2
-
 Homo sapiens N-benzylnicotinamide + reduced coenzyme Q2
-
 ?
1.10.5.1 nicotinamide riboside + menadiol
-
 Homo sapiens dihydronicotinamide riboside + menadione
-
 ?
1.10.5.1 nicotinamide riboside + reduced 2,6-dichlorophenolindophenol
-
 Homo sapiens dihydronicotinamide riboside + 2,6-dichlorophenolindophenol
-
 ?
1.10.5.1 nicotinamide riboside + reduced coenzyme Q0
-
 Homo sapiens dihydronicotinamide riboside + coenzyme Q0
-
 ?
1.10.5.1 nicotinamide riboside + reduced coenzyme Q1
-
 Homo sapiens dihydronicotinamide riboside + coenzyme Q1
-
 ?
1.10.5.1 nicotinamide riboside + reduced coenzyme Q2
-
 Homo sapiens dihydronicotinamide riboside + coenzyme Q2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.10.5.1 QR2
-
 Homo sapiens
1.10.5.1 quinone reductase 2
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.10.5.1 FAD
-
 Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.10.5.1 0.0000007
-
 using nicotinamide riboside and menadione as substrates, at 25°C, in 50 mM Tris-HCl, pH 8.5 Homo sapiens S29434
1.10.5.1 0.000005
-
 using nicotinamide riboside and coenzyme Q2 as substrates, at 25°C, in 50 mM Tris-HCl, pH 8.5 Homo sapiens S29434
1.10.5.1 0.0000067
-
 using N-benzyl dihydronicotinamide and menadione as substrates, at 25°C, in 50 mM Tris-HCl, pH 8.5 Homo sapiens S29434
1.10.5.1 0.000016
-
 using nicotinamide riboside and menadione as substrates, at 25°C, in 50 mM Tris-HCl, pH 8.5 Homo sapiens chrysoeriol
1.10.5.1 0.000076
-
 using nicotinamide riboside and coenzyme Q2 as substrates, at 25°C, in 50 mM Tris-HCl, pH 8.5 Homo sapiens chrysoeriol
1.10.5.1 0.000079
-
 using N-benzyl dihydronicotinamide and coenzyme Q2 as substrates, at 25°C, in 50 mM Tris-HCl, pH 8.5 Homo sapiens S29434
1.10.5.1 0.000129
-
 using nicotinamide riboside and menadione as substrates, at 25°C, in 50 mM Tris-HCl, pH 8.5 Homo sapiens resveratrol
1.10.5.1 0.000143
-
 using N-benzyl dihydronicotinamide and menadione as substrates, at 25°C, in 50 mM Tris-HCl, pH 8.5 Homo sapiens resveratrol
1.10.5.1 0.00016
-
 using N-benzyl dihydronicotinamide and menadione as substrates, at 25°C, in 50 mM Tris-HCl, pH 8.5 Homo sapiens chrysoeriol
1.10.5.1 0.00082
-
 using N-benzyl dihydronicotinamide and coenzyme Q2 as substrates, at 25°C, in 50 mM Tris-HCl, pH 8.5 Homo sapiens chrysoeriol
1.10.5.1 0.0012
-
 using nicotinamide riboside and menadione as substrates, at 25°C, in 50 mM Tris-HCl, pH 8.5 Homo sapiens S26695
1.10.5.1 0.0025
-
 using N-benzyl dihydronicotinamide and coenzyme Q2 as substrates, at 25°C, in 50 mM Tris-HCl, pH 8.5 Homo sapiens S26695
1.10.5.1 0.0028
-
 using nicotinamide riboside and menadione as substrates, at 25°C, in 50 mM Tris-HCl, pH 8.5 Homo sapiens Melatonin
1.10.5.1 0.0029
-
 using nicotinamide riboside and coenzyme Q2 as substrates, at 25°C, in 50 mM Tris-HCl, pH 8.5 Homo sapiens resveratrol
1.10.5.1 0.0126
-
 using nicotinamide riboside and coenzyme Q2 as substrates, at 25°C, in 50 mM Tris-HCl, pH 8.5 Homo sapiens S26695
1.10.5.1 0.0151
-
 using N-benzyl dihydronicotinamide and menadione as substrates, at 25°C, in 50 mM Tris-HCl, pH 8.5 Homo sapiens S26695
1.10.5.1 0.0397
-
 using nicotinamide riboside and coenzyme Q2 as substrates, at 25°C, in 50 mM Tris-HCl, pH 8.5 Homo sapiens Melatonin
1.10.5.1 0.0415
-
 using N-benzyl dihydronicotinamide and menadione as substrates, at 25°C, in 50 mM Tris-HCl, pH 8.5 Homo sapiens Melatonin
1.10.5.1 0.07
-
 using N-benzyl dihydronicotinamide and coenzyme Q2 as substrates, at 25°C, in 50 mM Tris-HCl, pH 8.5 Homo sapiens resveratrol