Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Yang, Q.; Gong, Z.J.; Zhou, Y.; Yuan, J.Q.; Cheng, J.; Tian, L.; Li, S.; Lin, X.D.; Xu, R.; Zhu, Z.R.; Mao, C.
    Role of Drosophila alkaline ceramidase (Dacer) in Drosophila development and longevity (2010), Cell. Mol. Life Sci., 67, 1477-1490.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.23 additional information Bac-Dacer microsomes cause a twofold increase in ceramidase activity on D-e-C6, D-e-C12, D-e-C16, D-e-C18, and D-e-C24:1-ceramide at pH 9.0 Drosophila melanogaster

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.23 the Dacer/brainwashing or BWA gene CG13969 localizes to the 38B2-3 region of the left arm of the second chromosome, DNA and amino acid sequence determination and analysis, functional overexpression in High Five insect cells Drosophila melanogaster

Protein Variants

EC Number Protein Variants Comment Organism
3.5.1.23 additional information insertional mutagenesis leading to enzyme inactivation, transposon location of the dacer mutant, overview. The mutation increases the levels of ceamides in both the pupal and adult stages, and increases pre-adult development time, lifespan, and anti-oxidative stress capacity, overview Drosophila melanogaster

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.23 membrane bound Drosophila melanogaster 16020
-
3.5.1.23 mitochondrion
-
 Drosophila melanogaster 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.23 Ca2+ required Drosophila melanogaster

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.23 34000
-
 x * 34000, recombinant enzyme, SDS-PAGE Drosophila melanogaster

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.23 ceramide + H2O Drosophila melanogaster
-
 sphingosine + fatty acid
-
 ?
3.5.1.23 ceramide + H2O Drosophila melanogaster BL-18012
-
 sphingosine + fatty acid
-
 ?
3.5.1.23 additional information Drosophila melanogaster ceramidases are a group of enzymes that catalyze the hydrolysis of ceramides to generate sphingosine, which is phosphorylated to form sphingosine 1-phosphate ?
-
 ?
3.5.1.23 additional information Drosophila melanogaster BL-18012 ceramidases are a group of enzymes that catalyze the hydrolysis of ceramides to generate sphingosine, which is phosphorylated to form sphingosine 1-phosphate ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.23 Drosophila melanogaster Q9VIP7 gene CG13969
-
3.5.1.23 Drosophila melanogaster BL-18012 Q9VIP7 gene CG13969
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.23 recombinant enzyme from High Five insect cells Drosophila melanogaster

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.23 midgut high mRNA levels Drosophila melanogaster
-
3.5.1.23 additional information high mRNA levels in the pupal stage Drosophila melanogaster
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.23 ceramide + H2O
-
 Drosophila melanogaster sphingosine + fatty acid
-
 ?
3.5.1.23 ceramide + H2O ceramidase activity on D-e-C6, D-e-C12, D-e-C16, D-e-C18, and D-e-C24:1-ceramide Drosophila melanogaster sphingosine + fatty acid
-
 ?
3.5.1.23 ceramide + H2O
-
 Drosophila melanogaster BL-18012 sphingosine + fatty acid
-
 ?
3.5.1.23 ceramide + H2O ceramidase activity on D-e-C6, D-e-C12, D-e-C16, D-e-C18, and D-e-C24:1-ceramide Drosophila melanogaster BL-18012 sphingosine + fatty acid
-
 ?
3.5.1.23 additional information ceramidases are a group of enzymes that catalyze the hydrolysis of ceramides to generate sphingosine, which is phosphorylated to form sphingosine 1-phosphate Drosophila melanogaster ?
-
 ?
3.5.1.23 additional information ceramidases are a group of enzymes that catalyze the hydrolysis of ceramides to generate sphingosine, which is phosphorylated to form sphingosine 1-phosphate Drosophila melanogaster BL-18012 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.23 ? x * 34000, recombinant enzyme, SDS-PAGE Drosophila melanogaster

Synonyms

EC Number Synonyms Comment Organism
3.5.1.23 Dacer
-
 Drosophila melanogaster
3.5.1.23 Drosophila alkaline ceramidase
-
 Drosophila melanogaster

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.23 37
-
 assay at Drosophila melanogaster

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.23 8
-
-
 Drosophila melanogaster

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.23 6 9 assay range Drosophila melanogaster

General Information

EC Number General Information Comment Organism
3.5.1.23 metabolism Dacer plays a role in fly development and longevity by controlling the metabolism of ceramides Drosophila melanogaster
3.5.1.23 physiological function Dacer plays a role in fly development and longevity by controlling the metabolism of ceramides. Dacer inactivation delays Drosophila pre-adult development, while Dacer inactivation increases Drosophila lifespan Drosophila melanogaster