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Literature summary extracted from

  • Wang, Z.L.; Ying, S.H.; Feng, M.G.
    Gene cloning and catalysis features of a new mannitol-1-phosphate dehydrogenase (BbMPD) from Beauveria bassiana (2010), Carbohydr. Res., 345, 50-54.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.17 gene BbMPD, DNA and amino acid sequence determination and analysis, phylogenetic tree Beauveria bassiana

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.17 Ca2+ 35.9% inhibition at 10 mM of the reverse reaction Beauveria bassiana
1.1.1.17 Cu2+ 32.8% inhibition at 2 mM of the reverse reaction Beauveria bassiana
1.1.1.17 DTT 46.0% inhibition at 10 mM of the reverse reaction Beauveria bassiana
1.1.1.17 Li+ 24.4% inhibition at 50 mM of the reverse reaction Beauveria bassiana
1.1.1.17 Mg2+ 31.2% inhibition at 10 mM of the reverse reaction Beauveria bassiana
1.1.1.17 Mn2+ 31.3% inhibition at 50 mM of the reverse reaction Beauveria bassiana
1.1.1.17 additional information no or poor inhibition of the reverse reaction by EDTA and 2-mercaptoethanol Beauveria bassiana
1.1.1.17 SDS 80.1% inhibition at 1 mM of the reverse reaction Beauveria bassiana
1.1.1.17 Zn2+ 44.7% inhibition at 2 mM of the reverse reaction Beauveria bassiana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.17 D-mannitol 1-phosphate + NAD+ Beauveria bassiana
-
 D-fructose 6-phosphate + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.17 Beauveria bassiana C7FDI4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.17 native enzyme Beauveria bassiana

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.17 673.7
-
 pH 7.0, 37°C, purified enzyme Beauveria bassiana

Storage Stability

EC Number Storage Stability Organism
1.1.1.17 4°C, purified native enzyme, the enzyme retains high activity during storage for two weeks Beauveria bassiana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.17 D-mannitol 1-phosphate + NAD+
-
 Beauveria bassiana D-fructose 6-phosphate + NADH + H+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.17 BbMPD
-
 Beauveria bassiana
1.1.1.17 long-chain mannitol-1-phosphate dehydrogenase
-
 Beauveria bassiana
1.1.1.17 mannitol-1-phosphate dehydrogenase
-
 Beauveria bassiana
1.1.1.17 MPD
-
 Beauveria bassiana
1.1.1.17 NAD+-dependent mannitol-1-phosphate dehydrogenase
-
 Beauveria bassiana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.17 37
-
-
 Beauveria bassiana

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.17 15 45 activity range, profile, overview Beauveria bassiana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.17 7
-
-
 Beauveria bassiana

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.17 3 10 activity range, profile, overview Beauveria bassiana

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.17 NAD+
-
 Beauveria bassiana
1.1.1.17 NADH
-
 Beauveria bassiana

Expression

EC Number Organism Comment Expression
1.1.1.17 Beauveria bassiana induction of the enzyme in cell culture by isopropyl 1-thio-beta-D-galactopyranoside up

General Information

EC Number General Information Comment Organism
1.1.1.17 physiological function crucial role of BbMPD in the mannitol biosynthesis of Beauveria bassiana Beauveria bassiana

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.17 1310
-
 D-fructose 6-phosphate pH 7.0, 37°C Beauveria bassiana