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Literature summary extracted from

  • Javvadi, P.; Hertan, L.; Kosoff, R.; Datta, T.; Kolev, J.; Mick, R.; Tuttle, S.W.; Koumenis, C.
    Thioredoxin reductase-1 mediates curcumin-induced radiosensitization of squamous carcinoma cells (2010), Cancer Res., 70, 1941-1950.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.1.9 additional information tetrahydrocurcumin has no significant effect on TxnRd activity at doses of up to 0.05 mM Homo sapiens
1.8.1.9 trans,trans-curcumin
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.1.9 cytosol
-
 Homo sapiens 5829
-

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.9 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.1.9 FaDu cell
-
 Homo sapiens
-
1.8.1.9 HEK-293 cell HEK-293 cells show low basal levels of TxnRd1 Homo sapiens
-
1.8.1.9 HeLa cell
-
 Homo sapiens
-
1.8.1.9 keratinocyte
-
 Homo sapiens
-
1.8.1.9 MSK-Leuk1 cell
-
 Homo sapiens
-
1.8.1.9 SiHa cell
-
 Homo sapiens
-
1.8.1.9 squamous cell carcinoma cell
-
 Homo sapiens
-
1.8.1.9 UM-SCC-1 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.9 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+
-
 Homo sapiens 2-nitro-5-thiobenzoate + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.1.9 thioredoxin reductase
-
 Homo sapiens
1.8.1.9 Txnrd1
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.9 FAD
-
 Homo sapiens
1.8.1.9 NADPH
-
 Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.8.1.9 0.01
-
 pH and temperature not specified in the publication Homo sapiens trans,trans-curcumin

Expression

EC Number Organism Comment Expression
1.8.1.9 Homo sapiens TxnRd1 expression is elevated by more than 50fold in FaDu and HeLa cells and by more than 20fold in SCC-1 cells compared with either MSK-Leuk1 cells or keratinocytes. TxnRd activity is about 20fold higher in FaDu and HeLa cells and about 7fold higher in SCC-1 cells compared with that in keratinocytes or MSK-Leuk1 cells up

General Information

EC Number General Information Comment Organism
1.8.1.9 malfunction stable knockdown of TxnRd1 in both HeLa and FaDu cells nearly abolishes curcumin-mediated radiosensitization. TxnRd1 knockdown cells show decreased radiation-induced reactive oxygen species and sustained extracellular signal-regulated kinase 1/2 activation Homo sapiens
1.8.1.9 physiological function thioredoxin reductase-1 mediates curcumin-induced radiosensitization of squamous carcinoma cells. Overexpressing catalytically active TxnRd1 in HEK-293 cells, with low basal levels of TxnRd1, increases their sensitivity to curcumin alone and to the combination of curcumin and ionizing radiation Homo sapiens