EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.24.87 | expression of wild-type and mutant ADAMTS13 in HEK-293 cells | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.4.24.87 | E663A | site-directed mutagenesis | Homo sapiens |
3.4.24.87 | E664A | site-directed mutagenesis | Homo sapiens |
3.4.24.87 | G662A | site-directed mutagenesis | Homo sapiens |
3.4.24.87 | R659A | site-directed mutagenesis | Homo sapiens |
3.4.24.87 | R660A | site-directed mutagenesis | Homo sapiens |
3.4.24.87 | R660A/Y661A | site-directed mutagenesis | Homo sapiens |
3.4.24.87 | R660A/Y661A/Y665A | site-directed mutagenesis, the ADAMTS13 variant, i.e. ADAMTS13-RYY, shows a 12fold reduced catalytic efficiency arising from over 25fold reduced substrate binding | Homo sapiens |
3.4.24.87 | R660A/Y665A | site-directed mutagenesis | Homo sapiens |
3.4.24.87 | Y658A | site-directed mutagenesis | Homo sapiens |
3.4.24.87 | Y661A | site-directed mutagenesis | Homo sapiens |
3.4.24.87 | Y661A/Y665A | site-directed mutagenesis | Homo sapiens |
3.4.24.87 | Y665A | site-directed mutagenesis | Homo sapiens |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.4.24.87 | Ca2+ | required | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.24.87 | von Willebrand factor + H2O | Homo sapiens | - |
? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.24.87 | Homo sapiens | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.24.87 | recombinant wild-type and mutant ADAMTS13 from HEK-293 cells by nickel affinity chromatography | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.24.87 | additional information | binding specificities of wild-type ADAMTS13 and ADAMTS13-RYY to von Willebrand factor115, von Willebrand factor106, and full-length von Willebrand factor, overview | Homo sapiens | ? | - |
? | |
3.4.24.87 | von Willebrand factor + H2O | - |
Homo sapiens | ? | - |
? | |
3.4.24.87 | von Willebrand factor + H2O | recombinant substrate stably expressed in HEK293 cells, the ADAMTS13 metalloprotease domain cleaves the von Willebrand factor A2 domain at the Y1605-M1606 scissile bond | Homo sapiens | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.24.87 | ADAMTS13 | - |
Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.24.87 | 37 | - |
assay at | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.24.87 | 7.8 | - |
assay at | Homo sapiens |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.4.24.87 | additional information | auto-antibodies, directed toward the spacer domain of ADAMTS13 involving region Y658-Y665 , cause ADAMTS13 deficiency and, as a result, acquired thrombotic thrombocytopenic purpura | Homo sapiens |