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Literature summary extracted from

  • Raife, T.J.; Cao, W.; Atkinson, B.S.; Bedell, B.; Montgomery, R.R.; Lentz, S.R.; Johnson, G.F.; Zheng, X.L.
    Leukocyte proteases cleave von Willebrand factor at or near the ADAMTS13 cleavage site (2009), Blood, 114, 1666-1674.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.87 stable expression of His-tagged ADAMTS13 in HEK-293 cells Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.87 0.0058
-
 FRETSVWF73 peptide pH 7.5, 37°C, recombinant ADAMTS13 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.87 extracellular
-
 Homo sapiens
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.87 Ca2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.87 von Willebrand factor + H2O Homo sapiens
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.87 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.87 recombinant His-tagged ADAMTS13 from HEK-293 cells by anion exchange chromatography, Ni affinity chromatography, and gel filtration Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.87 plasma
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.87 FRETSVWF73 peptide + H2O a von Willebrand factor-derived fluorescein-labeled peptide substrate Homo sapiens ?
-
 ?
3.4.24.87 von Willebrand factor + H2O
-
 Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.24.87 ADAMTS13
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.87 37
-
 assay at Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.24.87 4.43
-
 FRETSVWF73 peptide pH 7.5, 37°C, recombinant ADAMTS13 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.87 7.5
-
 assay at Homo sapiens

General Information

EC Number General Information Comment Organism
3.4.24.87 additional information under denaturing and fluid shear stress conditions multiple leukocyte proteases cleave von Willebrand factor predominantly in the central A2 domain. Activated neutrophils, but not normal neutrophils, retain von Willebrand factor-cleaving activity in the presence of plasma inhibitors, suggesting that leukocyte proteases may regulate von Willebrand factor function under physiologic conditions, kinetics and reaction products, overview Homo sapiens
3.4.24.87 physiological function importance of ADAMTS13 metalloprotease in von Willebrand factor regulation and association between severe deficiency of ADAMTS13 and thrombotic thrombocytopenic purpura. However, ADAMTS13 activity levels do not always correlate with the clinical course of TTP, suggesting that other proteases, e.g. elastase or proteinase 3 expressed on leukocyte surfaces, might be important in regulating von Willebrand factor Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.24.87 763.8
-
 FRETSVWF73 peptide pH 7.5, 37°C, recombinant ADAMTS13 Homo sapiens