BRENDA - Enzyme Database

The role of urease activity on biofilm formation by Staphylococcus sp. T-02 isolated from the toilet bowl

Oki, K.; Washio, K.; Matsui, D.; Kato, S.; Hirata, Y.; Morikawa, M.; Biosci. Biotechnol. Biochem. 74, 583-589 (2010)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.1.5
Acetohydroxamic acid
inhibits urease activity and biofilm formation in a dose-dependent manner
Staphylococcus sp. T-02
3.5.1.5
Boric acid
inhibits urease activity and biofilm formation in a dose-dependent manner
Staphylococcus sp. T-02
3.5.1.5
Hydroxyurea
inhibits urease activity and biofilm formation in a dose-dependent manner
Staphylococcus sp. T-02
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.1.5
Staphylococcus sp. T-02
-
exhibits high urease activity and is capable of growing in urinary condition by forming robust biofilms
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.5.1.5
additional information
-
urease activites of Staphylococcus sp. T-02 cells grown in AU medium are higher. When urea is omitted from the AU medium, the overall activity is reduced up to 50%
Staphylococcus sp. T-02
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.5
urea + H2O
-
711522
Staphylococcus sp. T-02
CO2 + NH3
-
-
-
?
Temperature Optimum [░C]
EC Number
Temperature Optimum [░C]
Temperature Optimum Maximum [░C]
Commentary
Organism
3.5.1.5
37
-
assay at
Staphylococcus sp. T-02
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.1.5
7
-
assay at
Staphylococcus sp. T-02
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.1.5
Acetohydroxamic acid
inhibits urease activity and biofilm formation in a dose-dependent manner
Staphylococcus sp. T-02
3.5.1.5
Boric acid
inhibits urease activity and biofilm formation in a dose-dependent manner
Staphylococcus sp. T-02
3.5.1.5
Hydroxyurea
inhibits urease activity and biofilm formation in a dose-dependent manner
Staphylococcus sp. T-02
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.5.1.5
additional information
-
urease activites of Staphylococcus sp. T-02 cells grown in AU medium are higher. When urea is omitted from the AU medium, the overall activity is reduced up to 50%
Staphylococcus sp. T-02
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.5
urea + H2O
-
711522
Staphylococcus sp. T-02
CO2 + NH3
-
-
-
?
Temperature Optimum [░C] (protein specific)
EC Number
Temperature Optimum [░C]
Temperature Optimum Maximum [░C]
Commentary
Organism
3.5.1.5
37
-
assay at
Staphylococcus sp. T-02
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.1.5
7
-
assay at
Staphylococcus sp. T-02