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Literature summary extracted from

  • Machida, T.; Kato, E.; Ishibashi, A.; Sato, J.; Kawasaki, S.; Niimura, Y.; Honjoh, K.; Miyamoto, T.
    Expression pattern of a chloroplast NADPH-dependent thioredoxin reductase in Chlorella vulgaris during hardening and its interaction with 2-Cys peroxiredoxin (2009), Biosci. Biotechnol. Biochem., 73, 695-701.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.1.9 expressed in Escherichia coli BL21(DE3)pLysS cells Chlorella vulgaris

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.1.9 chloroplast
-
 Chlorella vulgaris 9507
-

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.9 Chlorella vulgaris B9ZYY5
-
-
1.8.1.9 Chlorella vulgaris Beijerinck IAM C-27 B9ZYY5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.9 Ni2+ affinity column chromatography Chlorella vulgaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.9 5,5'-dithiobis(2-nitrobenzoic acid) + dithiothreitol
-
 Chlorella vulgaris 2-nitro-5-thiobenzoate + oxidized dithiothreitol
-
 ?
1.8.1.9 5,5'-dithiobis(2-nitrobenzoic acid) + dithiothreitol
-
 Chlorella vulgaris Beijerinck IAM C-27 2-nitro-5-thiobenzoate + oxidized dithiothreitol
-
 ?
1.8.1.9 additional information NTRC shows both NADPH-dependent thioredoxin reductase and thioredoxin-like dithiol-disulfide oxidoreductase activities Chlorella vulgaris ?
-
 ?
1.8.1.9 additional information NTRC shows both NADPH-dependent thioredoxin reductase and thioredoxin-like dithiol-disulfide oxidoreductase activities Chlorella vulgaris Beijerinck IAM C-27 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.1.9 NTR
-
 Chlorella vulgaris
1.8.1.9 NTRC
-
 Chlorella vulgaris
1.8.1.9 thioredoxin reductase
-
 Chlorella vulgaris

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.9 FAD
-
 Chlorella vulgaris
1.8.1.9 NADPH dependent on Chlorella vulgaris

Expression

EC Number Organism Comment Expression
1.8.1.9 Chlorella vulgaris the transcript of NTRC increases throughout 24 h hardening, whereas the encoded protein amount and total NTR activity decrease once and the increase during hardening up

General Information

EC Number General Information Comment Organism
1.8.1.9 physiological function NTRC is maintained at a constant level during hardening and functions as an antioxidant with 2-Cys peroxiredoxin in the acquisition of freezing tolerance of Chlorella Chlorella vulgaris