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Literature summary extracted from

  • Ding, H.T.; Du, Y.Q.; Liu, D.F.; Li, Z.L.; Chen, X.J.; Zhao, Y.H.
    Cloning and expression in E. coli of an organic solvent-tolerant and alkali-resistant glucose 1-dehydrogenase from Lysinibacillus sphaericus G10 (2010), Biores. Technol., 102, 1528-1536.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.47 EDTA 10-100 mM, sodium phosphate buffer, pH 8.0 Lysinibacillus sphaericus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.47 expressed in Escherichia coli Lysinibacillus sphaericus
1.1.1.47 His-tagged version expressed in Escherichia coli BL21(DE3) Lysinibacillus sphaericus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.47 Ca2+ 60% inhibition at 50, 100 and 10 mM in sodium phosphate buffer at pH 8.0, Tris/HCl buffer at pH 8.0 and Tris/HCl buffer at pH 9.0, respectively Lysinibacillus sphaericus
1.1.1.47 Cd2+ more than 50% inhibition at 20 mM Lysinibacillus sphaericus
1.1.1.47 Co2+ more than 50% inhibition at 20 mM Lysinibacillus sphaericus
1.1.1.47 Cu2+ more than 50% inhibition at 20 mM Lysinibacillus sphaericus
1.1.1.47 Fe2+ complete inhibition at 10 mM Lysinibacillus sphaericus
1.1.1.47 Fe3+ complete inhibition at 10 mM Lysinibacillus sphaericus
1.1.1.47 Mn2+ more than 50% inhibition at 20 mM Lysinibacillus sphaericus
1.1.1.47 Zn2+ complete inhibition at 10 mM Lysinibacillus sphaericus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.47 0.072
-
 NADP+ pH 8.0, 25°C Lysinibacillus sphaericus
1.1.1.47 0.094
-
 NAD+ pH 8.0, 25°C Lysinibacillus sphaericus
1.1.1.47 5.1
-
 D-glucose with NADP+, pH 8.0, 25°C Lysinibacillus sphaericus
1.1.1.47 6
-
 D-glucose with NAD+, pH 8.0, 25°C Lysinibacillus sphaericus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.47 Cd2+ inhibits enzymatic activity Lysinibacillus sphaericus
1.1.1.47 Co2+ inhibits enzymatic activity Lysinibacillus sphaericus
1.1.1.47 Cu2+ inhibits enzymatic activity Lysinibacillus sphaericus
1.1.1.47 EDTA enhances activity Lysinibacillus sphaericus
1.1.1.47 Fe2+ inhibits enzymatic activity Lysinibacillus sphaericus
1.1.1.47 Fe3+ inhibits enzymatic activity Lysinibacillus sphaericus
1.1.1.47 K+ enhances activity Lysinibacillus sphaericus
1.1.1.47 K+ 50-200 mM, Tris/HCl buffer pH 9.0 Lysinibacillus sphaericus
1.1.1.47 Li+ enhances activity Lysinibacillus sphaericus
1.1.1.47 Li+ 50-200 mM, Tris/HCl buffer pH 9.0 Lysinibacillus sphaericus
1.1.1.47 Mg2+ enhances activity Lysinibacillus sphaericus
1.1.1.47 Mg2+ 1-200 mM, Tris/HCl buffer pH 8.0 Lysinibacillus sphaericus
1.1.1.47 Mn2+ inhibits enzymatic activity Lysinibacillus sphaericus
1.1.1.47 Na+ enhances activity Lysinibacillus sphaericus
1.1.1.47 Na+ 50-200 mM, Tris/HCl buffer pH 9.0 Lysinibacillus sphaericus
1.1.1.47 NH4+ enhances activity Lysinibacillus sphaericus
1.1.1.47 NH4+ 50-200 mM, Tris/HCl buffer pH 9.0 Lysinibacillus sphaericus
1.1.1.47 Zn2+ inhibits enzymatic activity Lysinibacillus sphaericus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.47 27800
-
 calculated from cDNA Lysinibacillus sphaericus
1.1.1.47 28000
-
 SDS-PAGE Lysinibacillus sphaericus
1.1.1.47 120000
-
 gel filtration Lysinibacillus sphaericus

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.1.1.47 Acetone 30%: nearly 100% activity after 1h at 25°C Lysinibacillus sphaericus
1.1.1.47 Acetone enzyme exhibited high tolerance to 30% acetone Lysinibacillus sphaericus
1.1.1.47 DMSO 60%: nearly 100% activity after 1h at 25°C Lysinibacillus sphaericus
1.1.1.47 DMSO enzyme exhibited high tolerance to 60% DMSO Lysinibacillus sphaericus
1.1.1.47 Ethanol 30%: nearly 100% activity after 1h at 25°C Lysinibacillus sphaericus
1.1.1.47 Ethanol enzyme exhibited high tolerance to 30% ethanol Lysinibacillus sphaericus
1.1.1.47 isopropanol 30%: nearly 100% activity after 1h at 25°C Lysinibacillus sphaericus
1.1.1.47 isopropanol enzyme exhibits high tolerance to 30% isopropanol Lysinibacillus sphaericus
1.1.1.47 Methanol 30%: nearly 100% activity after 1h at 25°C Lysinibacillus sphaericus
1.1.1.47 Methanol enzyme exhibited high tolerance to 30% methanol Lysinibacillus sphaericus
1.1.1.47 n-hexane 30%: nearly 100% activity after 1h at 25°C Lysinibacillus sphaericus
1.1.1.47 n-hexane enzyme exhibits high tolerance to 30% n-hexane Lysinibacillus sphaericus
1.1.1.47 n-hexanol 60%: nearly 100% activity after 1h at 25°C Lysinibacillus sphaericus
1.1.1.47 n-hexanol enzyme exhibited high tolerance to 60% n-hexanol Lysinibacillus sphaericus
1.1.1.47 n-propanol 10%: nearly 100% activity after 1h at 25°C Lysinibacillus sphaericus
1.1.1.47 n-propanol enzyme exhibits high tolerance to 10% n-propanol Lysinibacillus sphaericus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.47 Lysinibacillus sphaericus C5IFU0
-
-
1.1.1.47 Lysinibacillus sphaericus G10 C5IFU0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.47 immobilized metal ion affinity chromatography (Ni2+) Lysinibacillus sphaericus

Storage Stability

EC Number Storage Stability Organism
1.1.1.47 -20°C, 25 mM sodium phosphate buffer (pH 6.5) with 20% glycerol (w/v), 1 year, no loss of activity Lysinibacillus sphaericus
1.1.1.47 30°C, 25 mM sodium phosphate buffer (pH 6.5), 1 week Lysinibacillus sphaericus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.47 D-galactose + NAD+ relative activity: 17.3% Lysinibacillus sphaericus D-galactono-1,5-lactone + NADH + H+
-
 ?
1.1.1.47 D-galactose + NAD+ relative activity: 17.3% Lysinibacillus sphaericus G10 D-galactono-1,5-lactone + NADH + H+
-
 ?
1.1.1.47 D-glucose + NAD+ relative activity: 100% Lysinibacillus sphaericus D-glucono-1,5-lactone + NADH + H+
-
 ?
1.1.1.47 D-glucose + NAD+ about 20% activity with D-galactose, D-xylose, maltose Lysinibacillus sphaericus D-glucono-1,5-lactone + NADH + H+
-
 ?
1.1.1.47 D-glucose + NAD+ relative activity: 100% Lysinibacillus sphaericus G10 D-glucono-1,5-lactone + NADH + H+
-
 ?
1.1.1.47 D-glucose + NAD+ about 20% activity with D-galactose, D-xylose, maltose Lysinibacillus sphaericus G10 D-glucono-1,5-lactone + NADH + H+
-
 ?
1.1.1.47 D-glucose + NADP+
-
 Lysinibacillus sphaericus D-glucono-1,5-lactone + NADPH + H+
-
 ?
1.1.1.47 D-glucose + NADP+
-
 Lysinibacillus sphaericus G10 D-glucono-1,5-lactone + NADPH + H+
-
 ?
1.1.1.47 D-lactose + NAD+ relative activity: 4.2% Lysinibacillus sphaericus D-lactono-1,5-lactone + NADH + H+
-
 ?
1.1.1.47 D-maltose + NAD+ relative activity: 22.4% Lysinibacillus sphaericus ?
-
 ?
1.1.1.47 D-mannose + NAD+ relative activity: 7.6% Lysinibacillus sphaericus D-mannono-1,5-lactone + NADH + H+
-
 ?
1.1.1.47 D-xylose + NAD+ relative activity: 22.5% Lysinibacillus sphaericus D-xylono-1,5-lactone + NADH + H+
-
 ?
1.1.1.47 D-xylose + NAD+ relative activity: 22.5% Lysinibacillus sphaericus G10 D-xylono-1,5-lactone + NADH + H+
-
 ?
1.1.1.47 sucrose + NAD+ relative activity: 6.3% Lysinibacillus sphaericus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.47 homotetramer 4 * 28000, SDS-PAGE, 120000 kDa by gel filtration, calculated: 261 amino acid residues, 27.8 kDa Lysinibacillus sphaericus
1.1.1.47 homotetramer gel filtration, 4 * 28000 Lysinibacillus sphaericus

Synonyms

EC Number Synonyms Comment Organism
1.1.1.47 alkali-resistant glucose 1-dehydrogenase
-
 Lysinibacillus sphaericus
1.1.1.47 GDH
-
 Lysinibacillus sphaericus
1.1.1.47 glucose 1-dehydrogenase
-
 Lysinibacillus sphaericus
1.1.1.47 LsGDH
-
 Lysinibacillus sphaericus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.47 50
-
-
 Lysinibacillus sphaericus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.47 25 35 stable for 1 week at 30°C (pH 6.5), completely inactivated in 30 min at 55°C, limited protection by glycerol, 84% activity after 30 min at 65°C in the presence of 2 M NaCl, Lysinibacillus sphaericus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.47 93
-
 NAD+ pH 8.0, 25°C Lysinibacillus sphaericus
1.1.1.47 93
-
 NADP+ pH 8.0, 25°C Lysinibacillus sphaericus
1.1.1.47 115
-
 NAD+ pH 8.0, 25°C Lysinibacillus sphaericus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.47 9.5
-
-
 Lysinibacillus sphaericus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.47 4 10.5
-
 Lysinibacillus sphaericus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.47 6.5 10
-
 Lysinibacillus sphaericus
1.1.1.47 6.5 10.5 most stable at pH 8.5, retains more than 80% activity at pH 6.5-10.5 after 1 h at 25°C, more than 30% activity after 24 h at pH 10.0 Lysinibacillus sphaericus
1.1.1.47 10
-
 at pH 10 for 24 h enzyme retains 30% of its activity Lysinibacillus sphaericus