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Literature summary extracted from
- Enhancement of avermectin and ivermectin production by overexpression of the maltose ATP-binding cassette transporter in Streptomyces avermitilis (2010), Biores. Technol., 101, 9228-9235.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 7.5.2.1 | synthesis | overexpression of malEFG-a improves the utilization rate of starch, and thereby enhances avermectin production, which is useful for the improvement of commercial antibiotic production using starch as the main carbon source in the fermentation process | Streptomyces avermitilis |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 7.5.2.1 | genes malEFG-a, encoding the maltose ABC transporter system, transcriptional analysis of malE-a by RT-PCR, and malEFG-a gene replacement cloning | Streptomyces avermitilis |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 7.5.2.1 | additional information | a malEFG-a deletion mutant is unable to grow in a minimal medium with maltose as sole carbon source and produce avermectin. Maltose utilization and avermectin production are restored by introduction of a single copy of malEFG-a. Overexpression of malEFG-a improves the utilization rate of starch, and thereby enhances avermectin production | Streptomyces avermitilis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 7.5.2.1 | Streptomyces avermitilis | - |
contains the maltose ABC transporter system encoded by malEFG-a | - |
| 7.5.2.1 | Streptomyces avermitilis ATCC 31267 | - |
contains the maltose ABC transporter system encoded by malEFG-a | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 7.5.2.1 | additional information | - |
avermectin production by wild-type and malEFG-a utant strains, overview | Streptomyces avermitilis |
Expression
| EC Number | Organism | Comment | Expression |
|---|---|---|---|
| 7.5.2.1 | Streptomyces avermitilis | expression of malE-a is strongly repressed by glucose | down |
| 7.5.2.1 | Streptomyces avermitilis | expression of malE-a is induced by maltose | up |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 7.5.2.1 | additional information | a malEFG-a deletion mutant is unable to grow in a minimal medium with maltose as sole carbon source and produce avermectin. Maltose utilization and avermectin production are restored by introduction of a single copy of malEFG-a. Overexpression of malEFG-a improves the utilization rate of starch, and thereby enhances avermectin production | Streptomyces avermitilis |
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Release 2023.1 (January 2023)
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