Any feedback?
Literature summary extracted from
- Immobilized aculeacin A acylase from Actinoplanes utahensis: characterization of a novel biocatalyst (2010), Biores. Technol., 101, 4261-4268.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.5.1.70 | biotechnology | recombinant enzyme covalently immobilized onto several epoxy-activated supports in order to obtain a robust biocatalyst to be used in industrial bioreactors. The best biocatalyst is obtained by attaching the enzyme on Sepabeads EC-EP5 | Actinoplanes utahensis |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.5.1.70 | recombinant enzyme covalently immobilized onto several epoxy-activated supports in order to obtain a robust biocatalyst to be used in industrial bioreactors. The best biocatalyst is obtained by attaching the enzyme on Sepabeads EC-EP5 | Actinoplanes utahensis |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.1.70 | 0.45 | - |
penicillin K | recombinant immobilized enzyme, Vmax: 7.6 micromol of produced 6-aminopenicillanic acid min-1/g wet biocatalyst, pH 8, 45°C | Actinoplanes utahensis |  |
| 3.5.1.70 | 3.9 | - |
penicillin dihydroF | recombinant immobilized enzyme, Vmax: 1.45 micromol of produced 6-aminopenicillanic acid min-1/g wet biocatalyst, pH 8, 45°C | Actinoplanes utahensis | |
| 3.5.1.70 | 6.3 | - |
aculeacin A | recombinant immobilized enzyme, Vmax: 35.2 micromol of hydrolzed aculeacin A min-1/g wet biocatalyst, pH 7, 60°C | Actinoplanes utahensis | |
| 3.5.1.70 | 6.45 | - |
Penicillin V | recombinant immobilized enzyme, Vmax: 35.2 micromol of produced 6-aminopenicillanic acid min-1/g wet biocatalyst, pH 8, 45°C | Actinoplanes utahensis | |
| 3.5.1.70 | 9.5 | - |
penicillin F | recombinant immobilized enzyme, Vmax: 0.54 micromol of produced 6-aminopenicillanic acid min-1/g wet biocatalyst, pH 8, 45°C | Actinoplanes utahensis | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.70 | aculeacin A + H2O | Actinoplanes utahensis | - |
? + palmitic acid | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.1.70 | Actinoplanes utahensis | - |
- |
- |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.70 | additional information | - |
35.2 U/g wet carrier recombinant immobilized enzyme, hydrolysis of penicillin V, pH 8, 45°C | Actinoplanes utahensis |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.5.1.70 | the recombinant immobilized enzyme can be recycled for at least 30 consecutive batch reactions at pH 8.0 and 45°C without loss of catalytic activity. Both soluble and immobilized enzyme apparently suffers no loss of enzymatic activity when stored at 45°C for at least 550 min | Actinoplanes utahensis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.70 | aculeacin A + H2O | - |
Actinoplanes utahensis | ? + palmitic acid | - |
? | |
| 3.5.1.70 | additional information | enzyme hydrolyzes the acyl moieties of antifungal echinocandin antibiotics, efficiently hydrolyzes penicillin V and natural aliphatic penicillins to yield 6-aminopenicillanic acid | Actinoplanes utahensis | ? | - |
? | |
| 3.5.1.70 | penicillin dihydroF + H2O | - |
Actinoplanes utahensis | 6-aminopenicillanic acid + hexanoic acid | - |
? | |
| 3.5.1.70 | penicillin F + H2O | - |
Actinoplanes utahensis | 6-aminopenicillanic acid + (3E)-hex-3-enoic acid | - |
? | |
| 3.5.1.70 | penicillin K + H2O | - |
Actinoplanes utahensis | 6-aminopenicillanic acid + octanoic acid | - |
? | |
| 3.5.1.70 | penicillin V + H2O | - |
Actinoplanes utahensis | 6-aminopenicillanic acid + phenoxyacetic acid | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.5.1.70 | aculeacin A acylase | - |
Actinoplanes utahensis |
| 3.5.1.70 | AuAAC | - |
Actinoplanes utahensis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.70 | 45 | - |
assay at | Actinoplanes utahensis |
| 3.5.1.70 | 60 | - |
assay at, substrate aculeacin A | Actinoplanes utahensis |
| 3.5.1.70 | 75 | - |
recombinant immobilized enzyme, highest activity with penicillin V, above the activity decreased very quickly as a result of the denaturation of the enzyme, pH 8.0 | Actinoplanes utahensis |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.70 | additional information | - |
half-life of the soluble enzyme at 65°C is estimate, t1/2: 477 min | Actinoplanes utahensis |
| 3.5.1.70 | 45 | - |
both soluble and immobilized enzyme apparently suffers no loss of enzymatic activity when stored at 45°C for at least 550 min | Actinoplanes utahensis |
| 3.5.1.70 | 65 | - |
the recombinant immobilized enzyme is highly thermostable since it suffers no loss of activity at 65°C and pH 8.0 during 3 h | Actinoplanes utahensis |
| 3.5.1.70 | 75 | - |
the recombinant immobilized enzyme is completely deactivated after its incubation during two hours at temperatures above 75°C | Actinoplanes utahensis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.70 | 7 | - |
assay at, substrate aculeacin A | Actinoplanes utahensis |
| 3.5.1.70 | 8 | - |
assay at | Actinoplanes utahensis |
| 3.5.1.70 | 8.5 | - |
recombinant immobilized enzyme, highest activity with penicillin V, above the activity decreases very quickly as a result of the denaturation of the enzyme, 40°C | Actinoplanes utahensis |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.70 | 6.5 | 11 | ca. 17 U/g wet carrier at pH 6.5 and ca. 20 U/g wet carrier at pH 11 | Actinoplanes utahensis |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.70 | 6.5 | 11 | the immobilized enzyme is stable at the whole pH range from pH 6.5 to 11. Stability is tested for the storage of the biocatalyst for two hours at different pH values at 4°C | Actinoplanes utahensis |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 3.5.1.70 | physiological function | enzyme hydrolyzes the acyl moieties of antifungal echinocandin antibiotics, efficiently hydrolyzes penicillin V and natural aliphatic penicillins to yield 6-aminopenicillanic acid | Actinoplanes utahensis |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
