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Literature summary extracted from

  • Ye, Q.; Yan, M.; Yao, Z.; Xu, L.; Cao, H.; Li, Z.; Chen, Y.; Li, S.; Bai, J.; Xiong, J.; Ying, H.; Ouyang, P.
    A new member of the short-chain dehydrogenases/reductases superfamily: purification, characterization and substrate specificity of a recombinant carbonyl reductase from Pichia stipitis (2009), Biores. Technol., 100, 6022-6027.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.184 expression in Escherichia coli Scheffersomyces stipitis

General Stability

EC Number General Stability Organism
1.1.1.184 compared to activity in acetate and Tris-HCl, carbonyl reductase shows higher activity using potassium phosphate as buffer at pH 5.5 or pH 7 Scheffersomyces stipitis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.184 2-mercaptoethanol 1 mM, 20.3% residual activity Scheffersomyces stipitis
1.1.1.184 sodium dodecylsulfate 0.1% w/v, complete loss of activity Scheffersomyces stipitis
1.1.1.184 Triton X-100 1% v/v, 88% residual activity Scheffersomyces stipitis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.184 35000
-
 x * 35000, SDS-PAGE Scheffersomyces stipitis

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.1.1.184 dimethyl sulfoxide 1% v/v, 88.4% residual activity Scheffersomyces stipitis
1.1.1.184 dimethylformamide 1% v/v, 46% residual activity Scheffersomyces stipitis
1.1.1.184 tetrahydrofuran 1% v/v, 88.4% residual activity Scheffersomyces stipitis

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.184 Scheffersomyces stipitis A3GF07
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.184 recombinant enzyme Scheffersomyces stipitis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.184 337
-
 pH 6.0, 30°C Scheffersomyces stipitis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.184 2,3-butanedione + NADPH + H+ 35.2% of the activity with ethyl 4-chloro-3-oxobutanoate Scheffersomyces stipitis ? + NADP+
-
 ?
1.1.1.184 2,3-pentanedione + NADPH + H+ 58.6% of the activity with ethyl 4-chloro-3-oxobutanoate Scheffersomyces stipitis ? + NADP+
-
 ?
1.1.1.184 3-hydroxybenzaldehyde + NADPH + H+ 7.1% of the activity with ethyl 4-chloro-3-oxobutanoate Scheffersomyces stipitis 3-hydroxybenzyl alcohol + NADP+
-
 ?
1.1.1.184 3-nitrobenzaldehyde + NADPH + H+ 7.0% of the activity with ethyl 4-chloro-3-oxobutanoate Scheffersomyces stipitis 3-nitrobenzyl alcohol + NADP+
-
 ?
1.1.1.184 ethyl 2-chloro-3-oxobutanoate + NADPH + H+ 135.7% of the activity with ethyl 4-chloro-3-oxobutanoate Scheffersomyces stipitis (S)-ethyl 2-chloro-3-hydroxybutanoate + NADP+
-
 ?
1.1.1.184 ethyl 3-oxobutanoate + NADPH + H+ 25.2% of the activity with ethyl 4-chloro-3-oxobutanoate Scheffersomyces stipitis ethyl 3-hydroxybutanoate + NADP+
-
 ?
1.1.1.184 ethyl 4-chloro-3-oxobutanoate + NADPH + H+
-
 Scheffersomyces stipitis (S)-ethyl 4-chloro-3-hydroxybutanoate + NADP+
-
 ?
1.1.1.184 isatin + NADPH + H+ 17.7% of the activity with ethyl 4-chloro-3-oxobutanoate Scheffersomyces stipitis 3-hydroxy-2-oxoindole + NADP+
-
 ?
1.1.1.184 methyl 2-chloro-3-oxobutanoate + NADPH + H+ 25.1% of the activity with ethyl 4-chloro-3-oxobutanoate Scheffersomyces stipitis (S)-methyl 2-chloro-3-hydroxybutanoate + NADP+
-
 ?
1.1.1.184 methyl 4-chloro-3-oxobutanoate + NADPH + H+ 25.4% of the activity with ethyl 4-chloro-3-oxobutanoate Scheffersomyces stipitis (S)-methyl 4-chloro-3-hydroxybutanoate + NADP+
-
 ?
1.1.1.184 additional information no activity for methyl-3-oxobutanoate and simple ketones and aldehydes such as benzaldehyde and acrolein. No substrate: pyridine-4-aldehyde, ethyl 4,4,4-trifluoro-3-oxobutanoate, pyridine-3-aldehyde or p-nitrobenzaldehyde Scheffersomyces stipitis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.184 ? x * 35000, SDS-PAGE Scheffersomyces stipitis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.184 30
-
-
 Scheffersomyces stipitis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.184 additional information
-
 sharp decrease in activity above 40°C Scheffersomyces stipitis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.184 35
-
 stable for 60 min Scheffersomyces stipitis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.184 6
-
-
 Scheffersomyces stipitis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.184 5.5 6.5 more than 90% of maximum activity Scheffersomyces stipitis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.184 4.5 7.5 4°C, maintains more than 80% of maximum activity for 48 h Scheffersomyces stipitis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.184 NADPH highly specific for NADPH as cofactor Scheffersomyces stipitis