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Literature summary extracted from

  • Xue, J.; Wang, A.; Zhou, C.; Shen, S.
    Penicillin acylase immobilization depending on macromolecular crowding and catalysis in aqueous-organic medium (2009), Bioprocess Biosyst. Eng., 32, 765-772.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.1.11 synthesis penicillin acylase is one of the key pharmaceutical enzymes in the production of polysynthetic beta-lactam antibiotics Priestia megaterium

Protein Variants

EC Number Protein Variants Comment Organism
3.5.1.11 additional information immobilization of the enzyme on mesocellular silica foams from 0.1 M phosphate buffer, pH 7.0, the immobilized enzyme activity depends on molecular crowding and applied to catalysis in non-aqueous medium, addition of dextran, method optimization, using different enzyme preparations, organic solvents and mesocellular silica foams-dextran mixtures, overview Priestia megaterium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.11 penicillin G + H2O Priestia megaterium
-
 phenylacetic acid + 6-aminopenicillanate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.11 Priestia megaterium
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.11 commercial preparation 1000 U/ml, 10 mg/ml Priestia megaterium
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.11 182
-
 immobilized enzyme, pH 8.0, 37°C Priestia megaterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.11 cephalosporin G + H2O
-
 Priestia megaterium phenylacetic acid + 7-aminodeacetoxycephalosporanate
-
 ?
3.5.1.11 penicillin G + H2O
-
 Priestia megaterium phenylacetic acid + 6-aminopenicillanate
-
 ?
3.5.1.11 penicillin G + H2O
-
 Priestia megaterium phenylacetic acid + 6-aminopenicillanate detection by reaction with p-dimethyl-aminobenzaldehyde ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.11 penicillin acylase
-
 Priestia megaterium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.11 37
-
 assay at Priestia megaterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.11 8
-
 assay at Priestia megaterium

General Information

EC Number General Information Comment Organism
3.5.1.11 additional information The enzyme catalyzes the hydrolysis of the amidic bonds of penicillin G and cephalosporin G to produce key components in the synthesis of beta-lactam antibiotics Priestia megaterium