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Literature summary extracted from

  • Okazaki, M.; Nimitkeatkai, H.; Muramatsu, T.; Aoyama, H.; Ueno, K.; Mizutani, M.; Hirai, N.; Kondo, S.; Ohnishi, T.; Todoroki, Y.
    Abscinazole-E1, a novel chemical tool for exploring the role of ABA 8-hydroxylase CYP707A (2011), Bioorg. Med. Chem., 19, 406-413.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.86 CYP701A, expression in Spodoptera frugiperda Sf9 cells using the baculovirus transfection method Oryza sativa
1.14.14.137 CYP707A3, coexpression with Arabidopsis thaliana P450 reductase ATR2 in Escherichia coli Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.14.86 (E)-2-(2-((1-(4-(3-hydroxy-4,4-dimethyl-2-(1H-1,2,4-triazol-1-yl)pent-1-en-1-yl)phenyl)-1H-1,2,3-triazol-4-yl)methoxy)ethoxy)ethyl 4-methylbenzenesulfonate i.e. abscinazole-E1, orUT1-E2Ts, or Abz-E1, a specific potent inhibitor of ABA 8'-hydroxylase, EC 1.14.13.93, but a weak inhibitor of CYP701A, both in vitro and in vivo Oryza sativa
1.14.14.86 abscinazole-F1
-
 Oryza sativa
1.14.14.86 DSI-505ME
-
 Oryza sativa
1.14.14.86 uniconazole i.e. UNI Oryza sativa
1.14.14.86 UT4
-
 Oryza sativa
1.14.14.137 (1E)-1-[4-(4-butyl-1H-1,2,3-triazol-1-yl)phenyl]-4,4-dimethyl-2-(1H-1,2,4-triazol-1-yl)pent-1-en-3-ol
-
 Arabidopsis thaliana
1.14.14.137 (1E,3S)-1-(4-chlorophenyl)-4,4-dimethyl-2-(1H-1,2,4-triazol-1-yl)pent-1-en-3-ol i.e. uniconazole Arabidopsis thaliana
1.14.14.137 (E)-2-(2-((1-(4-(3-hydroxy-4,4-dimethyl-2-(1H-1,2,4-triazol-1-yl)pent-1-en-1-yl)phenyl)-1H-1,2,3-triazol-4-yl)methoxy)ethoxy)ethyl 4-methylbenzenesulfonate i.e. abscinazole-E1, or UT1-E2Ts, or Abz-E1, a specific potent inhibitor of ABA 8'-hydroxylase, that is a weak inhibitor of ent-kaurene oxidase, CYP701A, EC 1.14.13.78, both in vitro and in vivo Arabidopsis thaliana
1.14.14.137 abscinazole-F1
-
 Arabidopsis thaliana
1.14.14.137 methyl (2E)-3-[1-[(1E)-1-(4-chlorophenyl)-3-hydroxy-4,4-dimethylpent-1-en-2-yl]-1H-imidazol-5-yl]prop-2-enoate
-
 Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.86 microsome
-
 Oryza sativa
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.86 additional information Oryza sativa CYP701A catalyzes the three-step oxidation of ent-kaurene to ent-kaurenoic acid, a biosynthetic precursor of the plant hormone gibberellin ?
-
 ?
1.14.14.86 additional information Oryza sativa Nipponbare CYP701A catalyzes the three-step oxidation of ent-kaurene to ent-kaurenoic acid, a biosynthetic precursor of the plant hormone gibberellin ?
-
 ?
1.14.14.137 (+)-abscisic acid + [reduced NADPH-hemoprotein reductase] + O2 Arabidopsis thaliana
-
 8'-hydroxyabscisate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.86 Oryza sativa
-
-
-
1.14.14.86 Oryza sativa Nipponbare
-
-
-
1.14.14.137 Arabidopsis thaliana
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.86 recombinant CYP701A partially from insect Sf9 cells in microsomes Oryza sativa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.86 ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
-
 Oryza sativa ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
 ?
1.14.14.86 ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
-
 Oryza sativa Nipponbare ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
 ?
1.14.14.86 additional information CYP701A catalyzes the three-step oxidation of ent-kaurene to ent-kaurenoic acid, a biosynthetic precursor of the plant hormone gibberellin Oryza sativa ?
-
 ?
1.14.14.86 additional information CYP701A catalyzes the three-step oxidation of ent-kaurene to ent-kaurenoic acid, a biosynthetic precursor of the plant hormone gibberellin Oryza sativa Nipponbare ?
-
 ?
1.14.14.137 (+)-abscisic acid + [reduced NADPH-hemoprotein reductase] + O2
-
 Arabidopsis thaliana 8'-hydroxyabscisate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.86 CYP701A
-
 Oryza sativa
1.14.14.137 ABA 8'-hydroxylase
-
 Arabidopsis thaliana
1.14.14.137 abscisic acid 8'-hydroxylase
-
 Arabidopsis thaliana
1.14.14.137 CYP707A
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.14.86 30
-
 assay at Oryza sativa
1.14.14.137 30
-
 assay at Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.86 7.25
-
 assay at Oryza sativa
1.14.14.137 7.25
-
 assay at Arabidopsis thaliana

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.14.14.86 0.00097
-
 (E)-2-(2-((1-(4-(3-hydroxy-4,4-dimethyl-2-(1H-1,2,4-triazol-1-yl)pent-1-en-1-yl)phenyl)-1H-1,2,3-triazol-4-yl)methoxy)ethoxy)ethyl 4-methylbenzenesulfonate pH 7.25, 30°C, recombinant enzyme Oryza sativa
1.14.14.137 0.000027
-
 (E)-2-(2-((1-(4-(3-hydroxy-4,4-dimethyl-2-(1H-1,2,4-triazol-1-yl)pent-1-en-1-yl)phenyl)-1H-1,2,3-triazol-4-yl)methoxy)ethoxy)ethyl 4-methylbenzenesulfonate pH 7.25, 30°C, recombinant enzyme Arabidopsis thaliana