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Literature summary extracted from

  • Nango, E.; Yamamoto, T.; Kumasaka, T.; Eguchi, T.
    Crystal structure of 3-isopropylmalate dehydrogenase in complex with NAD(+) and a designed inhibitor (2009), Bioorg. Med. Chem., 17, 7789-7794.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.85 crystal structure of Thermus thermophilus IPMDH in a ternary complex with NAD+ and the inhibitor ((2S,3S)-3-methylmercaptomalate) is determined at 2.8 A resolution. The inhibitor exists as a decarboxylated product with an enol/enolate form in the active site. The product interacts with Arg94, Asn102, Ser259, Glu270, and a water molecule hydrogen-bonding with Arg132. All interactions between the product and the enzyme are observed in the position associated with keto-enol tautomerization Thermus thermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.85 (2S,3S)-3-methylmercaptomalate strong competitive inhibitor Thermus thermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.85 Mg2+
-
Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.85 Thermus thermophilus Q5SIY4
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.85 (2R,3S)-3-isopropylmalate + NAD+
-
Thermus thermophilus (2S)-2-isopropyl-3-oxosuccinate + NADH + H+
-
?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.85 3-isopropylmalate dehydrogenase
-
Thermus thermophilus
1.1.1.85 IPMDH
-
Thermus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.85 60
-
assay at Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.85 7.8
-
assay at Thermus thermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.85 NADH
-
Thermus thermophilus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.85 0.000062
-
(2S,3S)-3-methylmercaptomalate pH 7.8, 60°C Thermus thermophilus