BRENDA - Enzyme Database

Mechanisms of catalysis and inhibition operative in the arginine deiminase from the human pathogen Giardia lamblia

Li, Z.; Kulakova, L.; Li, L.; Galkin, A.; Zhao, Z.; Nash, T.E.; Mariano, P.S.; Herzberg, O.; Dunaway-Mariano, D.; Bioorg. Chem. 37, 149-161 (2009)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.5.3.6
expressed in Escherichia coli BL21 Star(DE3) cells
Giardia intestinalis
Engineering
EC Number
Amino acid exchange
Commentary
Organism
3.5.3.6
C424A
catalytically inactive
Giardia intestinalis
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.3.6
(2S)-2-amino-5-(N''-cyanocarbamimidamido)pentanoic acid
-
Giardia intestinalis
3.5.3.6
agmatine
very weak, mixed-type inhibitor
Giardia intestinalis
3.5.3.6
additional information
Nomega-cyano-L-arginine does not inhibit Giardia lamblia arginine deiminase
Giardia intestinalis
3.5.3.6
S-nitroso-L-homocysteine
active site directed, irreversible inhibitor
Giardia intestinalis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.3.6
0.002
-
Nomega-amino-L-arginine
in 50 mM K+-HEPES (pH 7.5), at 25°C
Giardia intestinalis
3.5.3.6
0.16
-
L-arginine
in 50 mM K+-HEPES (pH 7.5), at 25°C
Giardia intestinalis
3.5.3.6
2.1
-
L-arginine ethyl ester
in 50 mM K+-HEPES (pH 7.5), at 25°C
Giardia intestinalis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.3.6
64189
-
2 * 64189, calculated from amino acid sequence
Giardia intestinalis
3.5.3.6
64190
-
2 * 64190, ESI-MS analysis
Giardia intestinalis
3.5.3.6
127000
-
gel filtration
Giardia intestinalis
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.3.6
Giardia intestinalis
Q27657
-
-
3.5.3.6
Giardia intestinalis isolate WB, clone 1267
Q27657
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.3.6
DEAE-cellulose column chromatography and butyl-Sepharose column chromatography
Giardia intestinalis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.3.6
L-arginine + H2O
-
711372
Giardia intestinalis
L-citrulline + NH3
-
-
-
?
3.5.3.6
L-arginine + H2O
-
711372
Giardia intestinalis isolate WB, clone 1267
L-citrulline + NH3
-
-
-
?
3.5.3.6
L-arginine ethyl ester + H2O
-
711372
Giardia intestinalis
?
-
-
-
?
3.5.3.6
L-arginine ethyl ester + H2O
-
711372
Giardia intestinalis isolate WB, clone 1267
?
-
-
-
?
3.5.3.6
additional information
does not accept agmatine, L-homoarginine, Nalpha-benzoyl-L-arginine ethyl ester as substrate
711372
Giardia intestinalis
?
-
-
-
-
3.5.3.6
additional information
does not accept agmatine, L-homoarginine, Nalpha-benzoyl-L-arginine ethyl ester as substrate
711372
Giardia intestinalis isolate WB, clone 1267
?
-
-
-
-
3.5.3.6
Nalpha-benzoyl-L-arginine + H2O
-
711372
Giardia intestinalis
?
-
-
-
?
3.5.3.6
Nalpha-benzoyl-L-arginine + H2O
-
711372
Giardia intestinalis isolate WB, clone 1267
?
-
-
-
?
3.5.3.6
Nomega-amino-L-arginine + H2O
-
711372
Giardia intestinalis
hydrazine + L-citrulline
-
-
-
?
3.5.3.6
Nomega-amino-L-arginine + H2O
-
711372
Giardia intestinalis isolate WB, clone 1267
hydrazine + L-citrulline
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.5.3.6
homodimer
2 * 64189, calculated from amino acid sequence; 2 * 64190, ESI-MS analysis
Giardia intestinalis
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.5.3.6
0.58
-
Nomega-amino-L-arginine
in 50 mM K+-HEPES (pH 7.5), at 25°C
Giardia intestinalis
3.5.3.6
2.3
-
L-arginine ethyl ester
in 50 mM K+-HEPES (pH 7.5), at 25°C
Giardia intestinalis
3.5.3.6
2.5
-
Nalpha-benzoyl-L-arginine
in 50 mM K+-HEPES (pH 7.5), at 25°C
Giardia intestinalis
3.5.3.6
2.6
-
L-arginine
in 50 mM K+-HEPES (pH 7.5), at 25°C
Giardia intestinalis
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.3.6
7.5
-
-
Giardia intestinalis
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.5.3.6
5.5
9
the kcat value is maximal from pH 5.5 to pH 9.0
Giardia intestinalis
3.5.3.6
6
8
the kcat_Km value is maximal between pH 6.0 and 8.0
Giardia intestinalis
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.5.3.6
40
-
S-nitroso-L-homocysteine
in 50 mM K+-HEPES (pH 7.5), at 25°C
Giardia intestinalis
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.3.6
expressed in Escherichia coli BL21 Star(DE3) cells
Giardia intestinalis
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
3.5.3.6
C424A
catalytically inactive
Giardia intestinalis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.3.6
(2S)-2-amino-5-(N''-cyanocarbamimidamido)pentanoic acid
-
Giardia intestinalis
3.5.3.6
agmatine
very weak, mixed-type inhibitor
Giardia intestinalis
3.5.3.6
additional information
Nomega-cyano-L-arginine does not inhibit Giardia lamblia arginine deiminase
Giardia intestinalis
3.5.3.6
S-nitroso-L-homocysteine
active site directed, irreversible inhibitor
Giardia intestinalis
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.5.3.6
40
-
S-nitroso-L-homocysteine
in 50 mM K+-HEPES (pH 7.5), at 25°C
Giardia intestinalis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.3.6
0.002
-
Nomega-amino-L-arginine
in 50 mM K+-HEPES (pH 7.5), at 25°C
Giardia intestinalis
3.5.3.6
0.16
-
L-arginine
in 50 mM K+-HEPES (pH 7.5), at 25°C
Giardia intestinalis
3.5.3.6
2.1
-
L-arginine ethyl ester
in 50 mM K+-HEPES (pH 7.5), at 25°C
Giardia intestinalis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.3.6
64189
-
2 * 64189, calculated from amino acid sequence
Giardia intestinalis
3.5.3.6
64190
-
2 * 64190, ESI-MS analysis
Giardia intestinalis
3.5.3.6
127000
-
gel filtration
Giardia intestinalis
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.3.6
DEAE-cellulose column chromatography and butyl-Sepharose column chromatography
Giardia intestinalis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.3.6
L-arginine + H2O
-
711372
Giardia intestinalis
L-citrulline + NH3
-
-
-
?
3.5.3.6
L-arginine + H2O
-
711372
Giardia intestinalis isolate WB, clone 1267
L-citrulline + NH3
-
-
-
?
3.5.3.6
L-arginine ethyl ester + H2O
-
711372
Giardia intestinalis
?
-
-
-
?
3.5.3.6
L-arginine ethyl ester + H2O
-
711372
Giardia intestinalis isolate WB, clone 1267
?
-
-
-
?
3.5.3.6
additional information
does not accept agmatine, L-homoarginine, Nalpha-benzoyl-L-arginine ethyl ester as substrate
711372
Giardia intestinalis
?
-
-
-
-
3.5.3.6
additional information
does not accept agmatine, L-homoarginine, Nalpha-benzoyl-L-arginine ethyl ester as substrate
711372
Giardia intestinalis isolate WB, clone 1267
?
-
-
-
-
3.5.3.6
Nalpha-benzoyl-L-arginine + H2O
-
711372
Giardia intestinalis
?
-
-
-
?
3.5.3.6
Nalpha-benzoyl-L-arginine + H2O
-
711372
Giardia intestinalis isolate WB, clone 1267
?
-
-
-
?
3.5.3.6
Nomega-amino-L-arginine + H2O
-
711372
Giardia intestinalis
hydrazine + L-citrulline
-
-
-
?
3.5.3.6
Nomega-amino-L-arginine + H2O
-
711372
Giardia intestinalis isolate WB, clone 1267
hydrazine + L-citrulline
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.5.3.6
homodimer
2 * 64189, calculated from amino acid sequence; 2 * 64190, ESI-MS analysis
Giardia intestinalis
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.5.3.6
0.58
-
Nomega-amino-L-arginine
in 50 mM K+-HEPES (pH 7.5), at 25°C
Giardia intestinalis
3.5.3.6
2.3
-
L-arginine ethyl ester
in 50 mM K+-HEPES (pH 7.5), at 25°C
Giardia intestinalis
3.5.3.6
2.5
-
Nalpha-benzoyl-L-arginine
in 50 mM K+-HEPES (pH 7.5), at 25°C
Giardia intestinalis
3.5.3.6
2.6
-
L-arginine
in 50 mM K+-HEPES (pH 7.5), at 25°C
Giardia intestinalis
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.3.6
7.5
-
-
Giardia intestinalis
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.5.3.6
5.5
9
the kcat value is maximal from pH 5.5 to pH 9.0
Giardia intestinalis
3.5.3.6
6
8
the kcat_Km value is maximal between pH 6.0 and 8.0
Giardia intestinalis
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
3.5.3.6
0.29
-
Nomega-amino-L-arginine
in 50 mM K+-HEPES (pH 7.5), at 25°C
Giardia intestinalis
3.5.3.6
2.3
-
Nalpha-benzoyl-L-arginine
in 50 mM K+-HEPES (pH 7.5), at 25°C
Giardia intestinalis
3.5.3.6
16
-
L-arginine
in 50 mM K+-HEPES (pH 7.5), at 25°C
Giardia intestinalis
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
3.5.3.6
0.29
-
Nomega-amino-L-arginine
in 50 mM K+-HEPES (pH 7.5), at 25°C
Giardia intestinalis
3.5.3.6
2.3
-
Nalpha-benzoyl-L-arginine
in 50 mM K+-HEPES (pH 7.5), at 25°C
Giardia intestinalis
3.5.3.6
16
-
L-arginine
in 50 mM K+-HEPES (pH 7.5), at 25°C
Giardia intestinalis