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Literature summary extracted from

  • Li, Z.; Kulakova, L.; Li, L.; Galkin, A.; Zhao, Z.; Nash, T.E.; Mariano, P.S.; Herzberg, O.; Dunaway-Mariano, D.
    Mechanisms of catalysis and inhibition operative in the arginine deiminase from the human pathogen Giardia lamblia (2009), Bioorg. Chem., 37, 149-161.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.3.6 expressed in Escherichia coli BL21 Star(DE3) cells Giardia intestinalis

Protein Variants

EC Number Protein Variants Comment Organism
3.5.3.6 C424A catalytically inactive Giardia intestinalis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.3.6 (2S)-2-amino-5-(N''-cyanocarbamimidamido)pentanoic acid
-
 Giardia intestinalis
3.5.3.6 agmatine very weak, mixed-type inhibitor Giardia intestinalis
3.5.3.6 additional information Nomega-cyano-L-arginine does not inhibit Giardia lamblia arginine deiminase Giardia intestinalis
3.5.3.6 S-nitroso-L-homocysteine active site directed, irreversible inhibitor Giardia intestinalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.3.6 0.002
-
 Nomega-amino-L-arginine in 50 mM K+-HEPES (pH 7.5), at 25°C Giardia intestinalis
3.5.3.6 0.16
-
 L-arginine in 50 mM K+-HEPES (pH 7.5), at 25°C Giardia intestinalis
3.5.3.6 2.1
-
 L-arginine ethyl ester in 50 mM K+-HEPES (pH 7.5), at 25°C Giardia intestinalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.3.6 64189
-
 2 * 64189, calculated from amino acid sequence Giardia intestinalis
3.5.3.6 64190
-
 2 * 64190, ESI-MS analysis Giardia intestinalis
3.5.3.6 127000
-
 gel filtration Giardia intestinalis

Organism

EC Number Organism UniProt Comment Textmining
3.5.3.6 Giardia intestinalis Q27657
-
-
3.5.3.6 Giardia intestinalis isolate WB, clone 1267 Q27657
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.3.6 DEAE-cellulose column chromatography and butyl-Sepharose column chromatography Giardia intestinalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.3.6 L-arginine + H2O
-
 Giardia intestinalis L-citrulline + NH3
-
 ?
3.5.3.6 L-arginine + H2O
-
 Giardia intestinalis isolate WB, clone 1267 L-citrulline + NH3
-
 ?
3.5.3.6 L-arginine ethyl ester + H2O
-
 Giardia intestinalis ?
-
 ?
3.5.3.6 L-arginine ethyl ester + H2O
-
 Giardia intestinalis isolate WB, clone 1267 ?
-
 ?
3.5.3.6 additional information does not accept agmatine, L-homoarginine, Nalpha-benzoyl-L-arginine ethyl ester as substrate Giardia intestinalis ?
-
 ?
3.5.3.6 additional information does not accept agmatine, L-homoarginine, Nalpha-benzoyl-L-arginine ethyl ester as substrate Giardia intestinalis isolate WB, clone 1267 ?
-
 ?
3.5.3.6 Nalpha-benzoyl-L-arginine + H2O
-
 Giardia intestinalis ?
-
 ?
3.5.3.6 Nalpha-benzoyl-L-arginine + H2O
-
 Giardia intestinalis isolate WB, clone 1267 ?
-
 ?
3.5.3.6 Nomega-amino-L-arginine + H2O
-
 Giardia intestinalis hydrazine + L-citrulline
-
 ?
3.5.3.6 Nomega-amino-L-arginine + H2O
-
 Giardia intestinalis isolate WB, clone 1267 hydrazine + L-citrulline
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.3.6 homodimer 2 * 64189, calculated from amino acid sequence Giardia intestinalis
3.5.3.6 homodimer 2 * 64190, ESI-MS analysis Giardia intestinalis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.3.6 0.58
-
 Nomega-amino-L-arginine in 50 mM K+-HEPES (pH 7.5), at 25°C Giardia intestinalis
3.5.3.6 2.3
-
 L-arginine ethyl ester in 50 mM K+-HEPES (pH 7.5), at 25°C Giardia intestinalis
3.5.3.6 2.5
-
 Nalpha-benzoyl-L-arginine in 50 mM K+-HEPES (pH 7.5), at 25°C Giardia intestinalis
3.5.3.6 2.6
-
 L-arginine in 50 mM K+-HEPES (pH 7.5), at 25°C Giardia intestinalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.3.6 7.5
-
-
 Giardia intestinalis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.3.6 5.5 9 the kcat value is maximal from pH 5.5 to pH 9.0 Giardia intestinalis
3.5.3.6 6 8 the kcat_Km value is maximal between pH 6.0 and 8.0 Giardia intestinalis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.5.3.6 40
-
 S-nitroso-L-homocysteine in 50 mM K+-HEPES (pH 7.5), at 25°C Giardia intestinalis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5.3.6 0.29
-
 Nomega-amino-L-arginine in 50 mM K+-HEPES (pH 7.5), at 25°C Giardia intestinalis
3.5.3.6 2.3
-
 Nalpha-benzoyl-L-arginine in 50 mM K+-HEPES (pH 7.5), at 25°C Giardia intestinalis
3.5.3.6 16
-
 L-arginine in 50 mM K+-HEPES (pH 7.5), at 25°C Giardia intestinalis