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Literature summary extracted from

  • Barillas, W.; Beerhues, L.
    3-Hydroxybenzoate:coenzyme A ligase from cell cultures of Centaurium erythraea: isolation and characterization (2000), Biol. Chem., 381, 155-160.
    View publication on PubMed

General Stability

EC Number General Stability Organism
6.2.1.37 glycerol, 5% does not affect enzyme stability Centaurium erythraea
6.2.1.37 the enzyme could be stored frozen for several months without appreciable loss of activity Centaurium erythraea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.37 0.0085
-
 CoA pH 7.5, 35°C Centaurium erythraea
6.2.1.37 0.0147
-
 3-hydroxybenzoate pH 7.5, 35°C Centaurium erythraea
6.2.1.37 0.229
-
 ATP pH 7.5, 35°C Centaurium erythraea

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.37 Mg2+ strictly dependent on Mg2+ or Mn2+ ions, with concentrations of 0.5 mM leading to maximum activity. No enzyme activity was found in the presence of Ca2+ Centaurium erythraea
6.2.1.37 Mn2+ strictly dependent on Mg2+ or Mn2+ ions, with concentrations of 0.5 mM leading to maximum activity. No enzyme activity was found in the presence of Ca2+ Centaurium erythraea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.37 50000
-
 gel filtration Centaurium erythraea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.37 ATP + 3-hydroxybenzoate + CoA Centaurium erythraea the enzyme is involved in the biosynthesis of xanthones AMP + diphosphate + 3-hydroxybenzoyl-CoA
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
6.2.1.37 Glycerol 5%, does not affect enzyme stability Centaurium erythraea

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.37 Centaurium erythraea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.37
-
 Centaurium erythraea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.2.1.37 cell suspension culture
-
 Centaurium erythraea
-

Storage Stability

EC Number Storage Stability Organism
6.2.1.37 4°C, crude extracts prepared with 0.1 M potassium phosphate buffer, pH 7.5, complete loss of activity within 24 h Centaurium erythraea
6.2.1.37 the enzyme could be stored frozen for several months without appreciable loss of activity Centaurium erythraea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.37 ATP + 3-hydroxybenzoate + CoA
-
 Centaurium erythraea AMP + diphosphate + 3-hydroxybenzoyl-CoA
-
 ?
6.2.1.37 ATP + 3-hydroxybenzoate + CoA the enzyme is involved in the biosynthesis of xanthones Centaurium erythraea AMP + diphosphate + 3-hydroxybenzoyl-CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
6.2.1.37 ? two polypeptides of 41500 Da and 40500 Da are detected by SDS-PAGE, difference in their apparent molecular masses can not be attributed to glycosylation Centaurium erythraea

Synonyms

EC Number Synonyms Comment Organism
6.2.1.37 3HBL
-
 Centaurium erythraea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.2.1.37 35
-
-
 Centaurium erythraea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.37 7.5
-
-
 Centaurium erythraea

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.2.1.37 6.5 8.5 pH 6.5: about 50% of maximal activity, pH 8.5: about 50% of maximal activity Centaurium erythraea