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Literature summary extracted from

  • Silveira, C.M.; Besson, S.; Moura, I.; Moura, J.J.; Almeida, M.G.
    Measuring the cytochrome C nitrite reductase activity-practical considerations on the enzyme assays (2010), Bioinorg. Chem. Appl., 2010, 634597.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.2.2 0.0027
-
 reduced phenosafranine apparent value, in 0.2 M phosphate buffer pH 7.6, at 37°C Desulfovibrio desulfuricans
1.7.2.2 0.0064
-
 reduced diquat apparent value, in 0.2 M phosphate buffer pH 7.6, at 37°C Desulfovibrio desulfuricans
1.7.2.2 0.015
-
 reduced methyl viologen apparent value, in 0.2 M phosphate buffer pH 7.6, at 37°C Desulfovibrio desulfuricans

Organism

EC Number Organism UniProt Comment Textmining
1.7.2.2 Desulfovibrio desulfuricans Q8VNU2 catalytic subunit
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.2.2 nitrite + reduced anthraquinone-2-sulfonate + H+ about 95% conversion Desulfovibrio desulfuricans NH3 + H2O + oxidized anthraquinone-2-sulfonate
-
 ?
1.7.2.2 nitrite + reduced diquat + H+ 100% conversion Desulfovibrio desulfuricans NH3 + H2O + oxidized diquat
-
 ?
1.7.2.2 nitrite + reduced indigo carmine + H+ 75% conversion Desulfovibrio desulfuricans NH3 + H2O + oxidized indigo carmine
-
 ?
1.7.2.2 nitrite + reduced methyl viologen + H+ about 98% conversion Desulfovibrio desulfuricans NH3 + H2O + oxidized methyl viologen
-
 ?
1.7.2.2 nitrite + reduced phenosafranine + H+ about 102% conversion Desulfovibrio desulfuricans NH3 + H2O + oxidized phenosafranine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.7.2.2 ccNiR
-
 Desulfovibrio desulfuricans
1.7.2.2 cytochrome c nitrite reductase
-
 Desulfovibrio desulfuricans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.7.2.2 79
-
 reduced phenosafranine apparent value, in 0.2 M phosphate buffer pH 7.6, at 37°C Desulfovibrio desulfuricans
1.7.2.2 738
-
 reduced methyl viologen apparent value, in 0.2 M phosphate buffer pH 7.6, at 37°C Desulfovibrio desulfuricans
1.7.2.2 1064
-
 reduced diquat apparent value, in 0.2 M phosphate buffer pH 7.6, at 37°C Desulfovibrio desulfuricans

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.2.2 heme
-
 Desulfovibrio desulfuricans

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.7.2.2 0.029
-
 reduced phenosafranine apparent value, in 0.2 M phosphate buffer pH 7.6, at 37°C Desulfovibrio desulfuricans
1.7.2.2 0.049
-
 reduced methyl viologen apparent value, in 0.2 M phosphate buffer pH 7.6, at 37°C Desulfovibrio desulfuricans
1.7.2.2 0.1664
-
 reduced diquat apparent value, in 0.2 M phosphate buffer pH 7.6, at 37°C Desulfovibrio desulfuricans