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Literature summary extracted from

  • Waidmann, M.; Bleichrodt, F.; Laslo, T.; Riedel, C.
    Bacterial luciferase reporters: the swiss army knife of molecular biology (2011), Bioeng. Bugs, 2, 8-16.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.14.14.3 analysis the enzyme is used as a reporter system tool for analysis of promoter and gene expression activity, overview Aliivibrio fischeri
1.14.14.3 analysis the enzyme is used as a reporter system tool for analysis of promoter and gene expression activity, overview Photobacterium phosphoreum
1.14.14.3 analysis the enzyme is used as a reporter system tool for analysis of promoter and gene expression activity, overview Photobacterium leiognathi
1.14.14.3 analysis the enzyme is used as a reporter system tool for analysis of promoter and gene expression activity, overview Vibrio harveyi
1.14.14.3 analysis the enzyme is used as a reporter system tool for analysis of promoter and gene expression activity, overview Photorhabdus laumondii subsp. laumondii
1.14.14.3 molecular biology the enzyme is used as a reporter system tool for analysis of promoter and gene expression activity, overview Aliivibrio fischeri
1.14.14.3 molecular biology the enzyme is used as a reporter system tool for analysis of promoter and gene expression activity, overview Photobacterium phosphoreum
1.14.14.3 molecular biology the enzyme is used as a reporter system tool for analysis of promoter and gene expression activity, overview Photobacterium leiognathi
1.14.14.3 molecular biology the enzyme is used as a reporter system tool for analysis of promoter and gene expression activity, overview Vibrio harveyi
1.14.14.3 molecular biology the enzyme is used as a reporter system tool for analysis of promoter and gene expression activity, overview Photorhabdus laumondii subsp. laumondii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.3 luxCDABE operon, genetic organization, overview Aliivibrio fischeri
1.14.14.3 luxCDABE operon, genetic organization, overview Photobacterium phosphoreum
1.14.14.3 luxCDABE operon, genetic organization, overview Photobacterium leiognathi
1.14.14.3 luxCDABE operon, genetic organization, overview Vibrio harveyi
1.14.14.3 luxCDABE operon, genetic organization, overview Photorhabdus laumondii subsp. laumondii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.3 an aldehyde + FMNH2 + O2 Aliivibrio fischeri
-
a carboxylate + FMN + H2O + hnu
-
?
1.14.14.3 an aldehyde + FMNH2 + O2 Photobacterium phosphoreum
-
a carboxylate + FMN + H2O + hnu
-
?
1.14.14.3 an aldehyde + FMNH2 + O2 Photobacterium leiognathi
-
a carboxylate + FMN + H2O + hnu
-
?
1.14.14.3 an aldehyde + FMNH2 + O2 Vibrio harveyi
-
a carboxylate + FMN + H2O + hnu
-
?
1.14.14.3 an aldehyde + FMNH2 + O2 Photorhabdus laumondii subsp. laumondii
-
a carboxylate + FMN + H2O + hnu
-
?
1.14.14.3 an aldehyde + FMNH2 + O2 Photorhabdus laumondii subsp. laumondii TT01
-
a carboxylate + FMN + H2O + hnu
-
?
1.14.14.3 an aldehyde + FMNH2 + O2 Photobacterium phosphoreum ATCC 11040
-
a carboxylate + FMN + H2O + hnu
-
?
1.14.14.3 an aldehyde + FMNH2 + O2 Photobacterium leiognathi ATCC 25521
-
a carboxylate + FMN + H2O + hnu
-
?
1.14.14.3 an aldehyde + FMNH2 + O2 Vibrio harveyi ATCC BAA1116
-
a carboxylate + FMN + H2O + hnu
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.3 Aliivibrio fischeri
-
genes lux A and luxB, of the luxCDABE operon, encoding the alpha and beta subunits of the luciferase
-
1.14.14.3 Photobacterium leiognathi
-
genes lux A and luxB, of the luxCDABE operon, encoding the alpha and beta subunits of the luciferase
-
1.14.14.3 Photobacterium leiognathi ATCC 25521
-
genes lux A and luxB, of the luxCDABE operon, encoding the alpha and beta subunits of the luciferase
-
1.14.14.3 Photobacterium phosphoreum
-
genes lux A and luxB, of the luxCDABE operon, encoding the alpha and beta subunits of the luciferase
-
1.14.14.3 Photobacterium phosphoreum ATCC 11040
-
genes lux A and luxB, of the luxCDABE operon, encoding the alpha and beta subunits of the luciferase
-
1.14.14.3 Photorhabdus laumondii subsp. laumondii
-
genes lux A and luxB, of the luxCDABE operon, encoding the alpha and beta subunits of the luciferase
-
1.14.14.3 Photorhabdus laumondii subsp. laumondii TT01
-
genes lux A and luxB, of the luxCDABE operon, encoding the alpha and beta subunits of the luciferase
-
1.14.14.3 Vibrio harveyi
-
genes lux A and luxB, of the luxCDABE operon, encoding the alpha and beta subunits of the luciferase
-
1.14.14.3 Vibrio harveyi ATCC BAA1116
-
genes lux A and luxB, of the luxCDABE operon, encoding the alpha and beta subunits of the luciferase
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.3 an aldehyde + FMNH2 + O2
-
Aliivibrio fischeri a carboxylate + FMN + H2O + hnu
-
?
1.14.14.3 an aldehyde + FMNH2 + O2
-
Photobacterium phosphoreum a carboxylate + FMN + H2O + hnu
-
?
1.14.14.3 an aldehyde + FMNH2 + O2
-
Photobacterium leiognathi a carboxylate + FMN + H2O + hnu
-
?
1.14.14.3 an aldehyde + FMNH2 + O2
-
Vibrio harveyi a carboxylate + FMN + H2O + hnu
-
?
1.14.14.3 an aldehyde + FMNH2 + O2
-
Photorhabdus laumondii subsp. laumondii a carboxylate + FMN + H2O + hnu
-
?
1.14.14.3 an aldehyde + FMNH2 + O2
-
Photorhabdus laumondii subsp. laumondii TT01 a carboxylate + FMN + H2O + hnu
-
?
1.14.14.3 an aldehyde + FMNH2 + O2
-
Photobacterium phosphoreum ATCC 11040 a carboxylate + FMN + H2O + hnu
-
?
1.14.14.3 an aldehyde + FMNH2 + O2
-
Photobacterium leiognathi ATCC 25521 a carboxylate + FMN + H2O + hnu
-
?
1.14.14.3 an aldehyde + FMNH2 + O2
-
Vibrio harveyi ATCC BAA1116 a carboxylate + FMN + H2O + hnu
-
?

Subunits

EC Number Subunits Comment Organism
1.14.14.3 heterodimer alpha-subunit, encoded by the luxA gene, and beta-subunit, encoded by the luxB gene Photobacterium phosphoreum

Synonyms

EC Number Synonyms Comment Organism
1.14.14.3 bacterial luciferase
-
Aliivibrio fischeri
1.14.14.3 bacterial luciferase
-
Photobacterium phosphoreum
1.14.14.3 bacterial luciferase
-
Photobacterium leiognathi
1.14.14.3 bacterial luciferase
-
Vibrio harveyi
1.14.14.3 bacterial luciferase
-
Photorhabdus laumondii subsp. laumondii

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.3 FMNH2
-
Aliivibrio fischeri
1.14.14.3 FMNH2
-
Photobacterium phosphoreum
1.14.14.3 FMNH2
-
Photobacterium leiognathi
1.14.14.3 FMNH2
-
Vibrio harveyi
1.14.14.3 FMNH2
-
Photorhabdus laumondii subsp. laumondii