EC Number | Cloned (Comment) | Organism |
---|---|---|
1.5.99.12 | expressed in Yarrowia lipolytica | Zea mays |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.5.99.12 | wild type and L492A mutant enzymes, hanging drop vapor diffusion method | Zea mays |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.5.99.12 | E381A | the mutation mimics the active site composition of naturally occurring isozymes CKO4/6 and CKO2/3 | Zea mays |
1.5.99.12 | E381S | the mutation mimics the active site composition of naturally occurring isozymes CKO4/6 and CKO2/3 | Zea mays |
1.5.99.12 | L492A | the mutant retains an unchanged pattern of selectivity to variously substituted ureas but it is much more susceptible to the inhibition by phenylureas as IC50 values are 10-50times lower than those for wild type enzyme | Zea mays |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.5.99.12 | (2,6-dichloro-pyridin-4-yl)-carbamic acid benzyl ester | - |
Zea mays | |
1.5.99.12 | (2-chloro-pyridin-4-yl)-carbamic acid 4-chloro-phenyl ester | - |
Zea mays | |
1.5.99.12 | 1-(2-chloro-1-oxidopyridin-4-yl)-3-phenylurea | - |
Zea mays | |
1.5.99.12 | 1-imidazo[1,2-a]pyridin-7-yl-3-phenylurea | - |
Zea mays | |
1.5.99.12 | N-(1,2,3-thidiazol-5-yl)-N'-phenylurea | - |
Zea mays | |
1.5.99.12 | N-(2,6-dichloro-pyridin-4-yl)-N'-benzylurea | - |
Zea mays | |
1.5.99.12 | N-(2,6-dichloro-pyridin-4-yl)-N'-phenoxyurea | - |
Zea mays | |
1.5.99.12 | N-(2,6-dichloro-pyridin-4-yl)-N'-phenylurea | - |
Zea mays | |
1.5.99.12 | N-(2,6-dichloropyridin-4-yl)-N'-benzyl-N'-methylurea | - |
Zea mays | |
1.5.99.12 | N-(2-amino-6-chloro-pyridin-4-yl)-N'-benzylurea | - |
Zea mays | |
1.5.99.12 | N-(2-amino-6-chloro-pyridin-4-yl)-N'-phenylurea | one of the most potent inhibitors of CKO | Zea mays | |
1.5.99.12 | N-(2-amino-pyridin-4-yl)-N'-phenylurea | one of the most potent inhibitors of CKO | Zea mays | |
1.5.99.12 | N-(2-chloro-6-methoxy-pyridin-4-yl)-N'-benzylurea | - |
Zea mays | |
1.5.99.12 | N-(2-chloro-pyridin-4-yl)-N'-benzylurea | - |
Zea mays | |
1.5.99.12 | N-(2-chloro-pyridin-4-yl)-N'-phenylurea | the compound binds in a planar conformation and competes for the same binding site with natural substrates | Zea mays |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.99.12 | 0.0011 | - |
N6-(2-isopentenyl)adenine | wild type enzyme, pH and temperature not specified in the publication | Zea mays | |
1.5.99.12 | 0.003 | - |
N6-(2-isopentenyl)adenine | mutant enzyme E381A, pH and temperature not specified in the publication | Zea mays | |
1.5.99.12 | 0.0032 | - |
N6-(2-isopentenyl)adenine | mutant enzyme E381S, pH and temperature not specified in the publication | Zea mays |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.99.12 | N6-(2-isopentenyl)adenine + FAD + H2O | Zea mays | - |
adenine + 3-methylbut-2-enal + FADH2 | - |
ir | |
1.5.99.12 | zeatin + FAD + H2O | Zea mays | - |
adenine + 4-hydroxy-3-methylbut-2-enal + FADH2 | - |
ir |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.99.12 | Zea mays | Q9T0N8 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.5.99.12 | - |
Zea mays |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.99.12 | N6-(2-isopentenyl)adenine + 2,6-dichlorophenolindophenol + H2O | - |
Zea mays | adenine + 3-methylbut-2-enal + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.5.99.12 | N6-(2-isopentenyl)adenine + FAD + H2O | - |
Zea mays | adenine + 3-methylbut-2-enal + FADH2 | - |
ir | |
1.5.99.12 | zeatin + FAD + H2O | - |
Zea mays | adenine + 4-hydroxy-3-methylbut-2-enal + FADH2 | - |
ir |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.5.99.12 | CKO1 | - |
Zea mays |
1.5.99.12 | cytokinin oxidase/dehydrogenase | - |
Zea mays |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.5.99.12 | FAD | - |
Zea mays |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
1.5.99.12 | 0.00004 | - |
mutant enzyme L492A, pH and temperature not specified in the publication | Zea mays | N-(2-amino-pyridin-4-yl)-N'-phenylurea | |
1.5.99.12 | 0.00004 | - |
mutant enzyme L492A, pH and temperature not specified in the publication | Zea mays | N-(2-amino-6-chloro-pyridin-4-yl)-N'-phenylurea | |
1.5.99.12 | 0.00008 | - |
mutant enzyme L492A, pH and temperature not specified in the publication | Zea mays | N-(2,6-dichloro-pyridin-4-yl)-N'-phenylurea | |
1.5.99.12 | 0.0001 | - |
mutant enzyme L492A, pH and temperature not specified in the publication | Zea mays | 1-imidazo[1,2-a]pyridin-7-yl-3-phenylurea | |
1.5.99.12 | 0.0002 | - |
mutant enzyme L492A, pH and temperature not specified in the publication | Zea mays | (2-chloro-pyridin-4-yl)-carbamic acid 4-chloro-phenyl ester | |
1.5.99.12 | 0.0004 | - |
mutant enzyme E381A, pH and temperature not specified in the publication | Zea mays | 1-imidazo[1,2-a]pyridin-7-yl-3-phenylurea | |
1.5.99.12 | 0.0004 | - |
mutant enzyme E381A, pH and temperature not specified in the publication | Zea mays | N-(2,6-dichloro-pyridin-4-yl)-N'-phenylurea | |
1.5.99.12 | 0.0005 | - |
mutant enzyme E381S, pH and temperature not specified in the publication | Zea mays | N-(2,6-dichloro-pyridin-4-yl)-N'-phenylurea | |
1.5.99.12 | 0.0006 | - |
mutant enzyme E381A, pH and temperature not specified in the publication | Zea mays | N-(2-amino-6-chloro-pyridin-4-yl)-N'-phenylurea | |
1.5.99.12 | 0.0007 | - |
mutant enzyme E381S, pH and temperature not specified in the publication | Zea mays | N-(2-amino-6-chloro-pyridin-4-yl)-N'-phenylurea | |
1.5.99.12 | 0.001 | - |
mutant enzyme E381S, pH and temperature not specified in the publication | Zea mays | 1-imidazo[1,2-a]pyridin-7-yl-3-phenylurea | |
1.5.99.12 | 0.0011 | - |
mutant enzyme E381A, pH and temperature not specified in the publication | Zea mays | N-(2,6-dichloropyridin-4-yl)-N'-benzyl-N'-methylurea | |
1.5.99.12 | 0.0015 | - |
mutant enzyme L492A, pH and temperature not specified in the publication | Zea mays | N-(2-chloro-pyridin-4-yl)-N'-benzylurea | |
1.5.99.12 | 0.0015 | - |
mutant enzyme L492A, pH and temperature not specified in the publication | Zea mays | N-(2,6-dichloropyridin-4-yl)-N'-benzyl-N'-methylurea | |
1.5.99.12 | 0.0018 | - |
wild type enzyme, pH and temperature not specified in the publication | Zea mays | N-(2-amino-6-chloro-pyridin-4-yl)-N'-phenylurea | |
1.5.99.12 | 0.002 | - |
mutant enzyme E381S, pH and temperature not specified in the publication | Zea mays | N-(2,6-dichloropyridin-4-yl)-N'-benzyl-N'-methylurea | |
1.5.99.12 | 0.002 | - |
mutant enzyme L492A, pH and temperature not specified in the publication | Zea mays | N-(2,6-dichloro-pyridin-4-yl)-N'-benzylurea | |
1.5.99.12 | 0.002 | - |
wild type enzyme, pH and temperature not specified in the publication | Zea mays | N-(2-amino-pyridin-4-yl)-N'-phenylurea | |
1.5.99.12 | 0.002 | - |
wild type enzyme, pH and temperature not specified in the publication | Zea mays | N-(2,6-dichloropyridin-4-yl)-N'-benzyl-N'-methylurea | |
1.5.99.12 | 0.003 | - |
mutant enzyme E381A, pH and temperature not specified in the publication | Zea mays | N-(2-chloro-pyridin-4-yl)-N'-benzylurea | |
1.5.99.12 | 0.003 | - |
mutant enzyme E381A, pH and temperature not specified in the publication | Zea mays | N-(2,6-dichloro-pyridin-4-yl)-N'-benzylurea | |
1.5.99.12 | 0.004 | - |
mutant enzyme E381A, pH and temperature not specified in the publication | Zea mays | (2,6-dichloro-pyridin-4-yl)-carbamic acid benzyl ester | |
1.5.99.12 | 0.004 | - |
mutant enzyme E381S, pH and temperature not specified in the publication | Zea mays | N-(2-chloro-pyridin-4-yl)-N'-benzylurea | |
1.5.99.12 | 0.004 | - |
mutant enzyme E381S, pH and temperature not specified in the publication | Zea mays | N-(2,6-dichloro-pyridin-4-yl)-N'-benzylurea | |
1.5.99.12 | 0.0045 | - |
wild type enzyme, pH and temperature not specified in the publication | Zea mays | N-(2,6-dichloro-pyridin-4-yl)-N'-phenylurea | |
1.5.99.12 | 0.005 | - |
mutant enzyme E381A, pH and temperature not specified in the publication | Zea mays | N-(2,6-dichloro-pyridin-4-yl)-N'-phenoxyurea | |
1.5.99.12 | 0.005 | - |
mutant enzyme L492A, pH and temperature not specified in the publication | Zea mays | N-(1,2,3-thidiazol-5-yl)-N'-phenylurea | |
1.5.99.12 | 0.005 | - |
mutant enzyme L492A, pH and temperature not specified in the publication | Zea mays | 1-(2-chloro-1-oxidopyridin-4-yl)-3-phenylurea | |
1.5.99.12 | 0.005 | - |
mutant enzyme L492A, pH and temperature not specified in the publication | Zea mays | N-(2,6-dichloro-pyridin-4-yl)-N'-phenoxyurea | |
1.5.99.12 | 0.006 | - |
mutant enzyme E381A, pH and temperature not specified in the publication | Zea mays | (2-chloro-pyridin-4-yl)-carbamic acid 4-chloro-phenyl ester | |
1.5.99.12 | 0.006 | - |
mutant enzyme E381S, pH and temperature not specified in the publication | Zea mays | (2-chloro-pyridin-4-yl)-carbamic acid 4-chloro-phenyl ester | |
1.5.99.12 | 0.006 | - |
mutant enzyme L492A, pH and temperature not specified in the publication | Zea mays | N-(2-amino-6-chloro-pyridin-4-yl)-N'-benzylurea | |
1.5.99.12 | 0.006 | - |
mutant enzyme L492A, pH and temperature not specified in the publication | Zea mays | N-(2-chloro-6-methoxy-pyridin-4-yl)-N'-benzylurea | |
1.5.99.12 | 0.006 | - |
wild type enzyme, pH and temperature not specified in the publication | Zea mays | 1-imidazo[1,2-a]pyridin-7-yl-3-phenylurea | |
1.5.99.12 | 0.007 | - |
mutant enzyme E381S, pH and temperature not specified in the publication | Zea mays | (2,6-dichloro-pyridin-4-yl)-carbamic acid benzyl ester | |
1.5.99.12 | 0.007 | - |
mutant enzyme L492A, pH and temperature not specified in the publication | Zea mays | (2,6-dichloro-pyridin-4-yl)-carbamic acid benzyl ester | |
1.5.99.12 | 0.008 | - |
mutant enzyme E381S, pH and temperature not specified in the publication | Zea mays | N-(2,6-dichloro-pyridin-4-yl)-N'-phenoxyurea | |
1.5.99.12 | 0.009 | - |
wild type enzyme, pH and temperature not specified in the publication | Zea mays | N-(2,6-dichloro-pyridin-4-yl)-N'-benzylurea | |
1.5.99.12 | 0.009 | - |
wild type enzyme, pH and temperature not specified in the publication | Zea mays | (2,6-dichloro-pyridin-4-yl)-carbamic acid benzyl ester | |
1.5.99.12 | 0.015 | - |
mutant enzyme E381A, pH and temperature not specified in the publication | Zea mays | N-(1,2,3-thidiazol-5-yl)-N'-phenylurea | |
1.5.99.12 | 0.015 | - |
mutant enzyme E381A, pH and temperature not specified in the publication | Zea mays | 1-(2-chloro-1-oxidopyridin-4-yl)-3-phenylurea | |
1.5.99.12 | 0.015 | - |
mutant enzyme E381A, pH and temperature not specified in the publication | Zea mays | N-(2-chloro-6-methoxy-pyridin-4-yl)-N'-benzylurea | |
1.5.99.12 | 0.017 | - |
mutant enzyme E381S, pH and temperature not specified in the publication | Zea mays | N-(1,2,3-thidiazol-5-yl)-N'-phenylurea | |
1.5.99.12 | 0.017 | - |
mutant enzyme E381S, pH and temperature not specified in the publication | Zea mays | 1-(2-chloro-1-oxidopyridin-4-yl)-3-phenylurea | |
1.5.99.12 | 0.018 | - |
wild type enzyme, pH and temperature not specified in the publication | Zea mays | N-(2-chloro-pyridin-4-yl)-N'-benzylurea | |
1.5.99.12 | 0.02 | - |
mutant enzyme E381S, pH and temperature not specified in the publication | Zea mays | N-(2-chloro-6-methoxy-pyridin-4-yl)-N'-benzylurea | |
1.5.99.12 | 0.02 | - |
wild type enzyme, pH and temperature not specified in the publication | Zea mays | N-(2,6-dichloro-pyridin-4-yl)-N'-phenoxyurea | |
1.5.99.12 | 0.022 | - |
wild type enzyme, pH and temperature not specified in the publication | Zea mays | N-(2-amino-6-chloro-pyridin-4-yl)-N'-benzylurea | |
1.5.99.12 | 0.022 | - |
wild type enzyme, pH and temperature not specified in the publication | Zea mays | N-(2-chloro-6-methoxy-pyridin-4-yl)-N'-benzylurea | |
1.5.99.12 | 0.024 | - |
mutant enzyme E381A, pH and temperature not specified in the publication | Zea mays | N-(2-amino-6-chloro-pyridin-4-yl)-N'-benzylurea | |
1.5.99.12 | 0.032 | - |
mutant enzyme E381A, pH and temperature not specified in the publication | Zea mays | N-(2-amino-pyridin-4-yl)-N'-phenylurea | |
1.5.99.12 | 0.032 | - |
mutant enzyme E381S, pH and temperature not specified in the publication | Zea mays | N-(2-amino-6-chloro-pyridin-4-yl)-N'-benzylurea | |
1.5.99.12 | 0.035 | - |
mutant enzyme E381S, pH and temperature not specified in the publication | Zea mays | N-(2-amino-pyridin-4-yl)-N'-phenylurea | |
1.5.99.12 | 0.035 | - |
mutant enzyme L492A, pH and temperature not specified in the publication | Zea mays | N-(2-chloro-pyridin-4-yl)-N'-phenylurea | |
1.5.99.12 | 0.039 | - |
wild type enzyme, pH and temperature not specified in the publication | Zea mays | (2-chloro-pyridin-4-yl)-carbamic acid 4-chloro-phenyl ester | |
1.5.99.12 | 0.042 | - |
wild type enzyme, pH and temperature not specified in the publication | Zea mays | N-(2-chloro-pyridin-4-yl)-N'-phenylurea | |
1.5.99.12 | 0.045 | - |
mutant enzyme E381A, pH and temperature not specified in the publication | Zea mays | N-(2-chloro-pyridin-4-yl)-N'-phenylurea | |
1.5.99.12 | 0.05 | - |
wild type enzyme, pH and temperature not specified in the publication | Zea mays | N-(1,2,3-thidiazol-5-yl)-N'-phenylurea | |
1.5.99.12 | 0.055 | - |
mutant enzyme E381S, pH and temperature not specified in the publication | Zea mays | N-(2-chloro-pyridin-4-yl)-N'-phenylurea | |
1.5.99.12 | 0.13 | - |
wild type enzyme, pH and temperature not specified in the publication | Zea mays | 1-(2-chloro-1-oxidopyridin-4-yl)-3-phenylurea |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.5.99.12 | physiological function | cytokinin oxidase/dehydrogenase irreversibly degrades the plant hormones cytokinins and thereby participates in their homeostasis | Zea mays |