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Literature summary extracted from

  • Jang, H.H.; Jamakhandi, A.P.; Sullivan, S.Z.; Yun, C.H.; Hollenberg, P.F.; Miller, G.P.
    Beta sheet 2-alpha helix C loop of cytochrome P450 reductase serves as a docking site for redox partners (2010), Biochim. Biophys. Acta, 1804, 1285-1293.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
1.6.2.4 D113A the mutation increases kcat approximately 2fold, but does not affect Km at the lowest ionic strength (10 mM), the mutant displays no change in catalytic efficiency compared to the wild type enzyme Rattus norvegicus
1.6.2.4 E115A/E116A the mutations increase kcat approximately 2fold, but does not affect Km at the lowest ionic strength (10 mM), the mutant displays a slight decrease in catalytic efficiency at higher ionic strengths due to a larger increase in Km than observed for kcat Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.2.4 0.0018
-
 oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide mutant enzyme E115A/E116A, using 10 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 0.002
-
 ferricytochrome c wild type enzyme, using 30 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 0.0021
-
 oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide mutant enzyme D113A, using 10 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 0.0022
-
 oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide wild type enzyme, using 10 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 0.0024
-
 ferricytochrome c mutant enzyme E115A/E116A, using 30 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 0.0025
-
 ferricytochrome c mutant enzyme D113A, using 30 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 0.0026
-
 oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide mutant enzyme D113A, using 30 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 0.0026
-
 oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide mutant enzyme E115A/E116A, using 30 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 0.0031
-
 oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide wild type enzyme, using 30 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 0.0036
-
 ferricytochrome c mutant enzyme E115A/E116A, using 60 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 0.0038
-
 oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide mutant enzyme E115A/E116A, using 60 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 0.0039
-
 oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide wild type enzyme, using 60 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 0.004
-
 ferricytochrome c wild type enzyme, using 60 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 0.0041
-
 oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide mutant enzyme D113A, using 60 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 0.0052
-
 ferricytochrome c mutant enzyme D113A, using 60 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 0.006
-
 ferricytochrome c mutant enzyme E115A/E116A, using 110 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 0.0062
-
 oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide wild type enzyme, using 110 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 0.0063
-
 oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide mutant enzyme D113A, using 110 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 0.0065
-
 oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide mutant enzyme E115A/E116A, using 110 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 0.0085
-
 ferricytochrome c wild type enzyme, using 110 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 0.0087
-
 oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide mutant enzyme D113A, using 210 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 0.01
-
 oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide wild type enzyme, using 210 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 0.011
-
 ferricytochrome c mutant enzyme D113A, using 110 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 0.011
-
 oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide mutant enzyme E115A/E116A, using 210 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 0.013
-
 ferricytochrome c mutant enzyme E115A/E116A, using 210 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 0.016
-
 ferricytochrome c mutant enzyme E115A/E116A, using 310 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 0.02
-
 ferricytochrome c wild type enzyme, using 210 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 0.023
-
 ferricytochrome c wild type enzyme, using 310 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 0.025
-
 ferricytochrome c mutant enzyme D113A, using 310 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 0.026
-
 ferricytochrome c mutant enzyme D113A, using 210 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.2.4 78000
-
 SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.6.2.4 2 ferricytochrome P450 + NADPH Rattus norvegicus
-
 2 ferrocytochrome P450 + NADP+ + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.6.2.4 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.2.4 2',5'-ADP agarose affinity column chromatography and DEAE resin column chromatography Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.2.4 2 ferricytochrome c + NADPH
-
 Rattus norvegicus 2 ferrocytochrome c + NADP+ + H+
-
 ?
1.6.2.4 2 ferricytochrome P450 + NADPH
-
 Rattus norvegicus 2 ferrocytochrome P450 + NADP+ + H+
-
 ?
1.6.2.4 NADPH + H+ + oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
-
 Rattus norvegicus NADP+ + reduced 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.6.2.4 CPR
-
 Rattus norvegicus
1.6.2.4 cytochrome P450 reductase
-
 Rattus norvegicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.6.2.4 5.83
-
 oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide wild type enzyme, using 10 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 8.5
-
 oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide wild type enzyme, using 30 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 10.5
-
 oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide mutant enzyme D113A, using 10 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 11.67
-
 oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide wild type enzyme, using 60 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 13
-
 oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide mutant enzyme E115A/E116A, using 10 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 13.83
-
 oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide mutant enzyme D113A, using 30 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 14.67
-
 ferricytochrome c wild type enzyme, using 60 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 16.67
-
 ferricytochrome c wild type enzyme, using 30 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 18.3
-
 oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide mutant enzyme E115A/E116A, using 30 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 18.3
-
 oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide wild type enzyme, using 110 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 20
-
 oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide mutant enzyme D113A, using 60 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 20
-
 ferricytochrome c wild type enzyme, using 110 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 23.3
-
 ferricytochrome c mutant enzyme D113A, using 60 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 25
-
 ferricytochrome c mutant enzyme D113A, using 30 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 25
-
 ferricytochrome c mutant enzyme E115A/E116A, using 30 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 25
-
 ferricytochrome c mutant enzyme E115A/E116A, using 60 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 25
-
 oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide mutant enzyme E115A/E116A, using 60 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 28.3
-
 oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide wild type enzyme, using 210 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 30
-
 oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide mutant enzyme D113A, using 110 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 33.33
-
 ferricytochrome c wild type enzyme, using 210 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 35
-
 ferricytochrome c wild type enzyme, using 310 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 36.7
-
 ferricytochrome c mutant enzyme D113A, using 110 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 36.7
-
 ferricytochrome c mutant enzyme E115A/E116A, using 110 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 38.3
-
 oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide mutant enzyme E115A/E116A, using 110 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 40
-
 oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide mutant enzyme D113A, using 210 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 55
-
 ferricytochrome c mutant enzyme D113A, using 310 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 56.67
-
 oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide mutant enzyme E115A/E116A, using 210 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 58.3
-
 ferricytochrome c mutant enzyme E115A/E116A, using 210 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 58.3
-
 ferricytochrome c mutant enzyme E115A/E116A, using 310 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 65
-
 ferricytochrome c mutant enzyme D113A, using 210 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.2.4 FAD
-
 Rattus norvegicus
1.6.2.4 FMN
-
 Rattus norvegicus
1.6.2.4 NADPH
-
 Rattus norvegicus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.6.2.4 1500
-
 ferricytochrome c wild type enzyme, using 310 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 1700
-
 ferricytochrome c wild type enzyme, using 210 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 2200
-
 ferricytochrome c mutant enzyme D113A, using 310 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 2300
-
 ferricytochrome c wild type enzyme, using 110 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 2500
-
 ferricytochrome c mutant enzyme D113A, using 210 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 2700
-
 oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide wild type enzyme, using 10 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 2700
-
 oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide wild type enzyme, using 30 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 2800
-
 oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide wild type enzyme, using 210 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 3000
-
 oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide wild type enzyme, using 110 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 3000
-
 oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide wild type enzyme, using 60 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 3300
-
 ferricytochrome c mutant enzyme D113A, using 110 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 3700
-
 ferricytochrome c mutant enzyme E115A/E116A, using 310 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 3700
-
 ferricytochrome c wild type enzyme, using 60 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 4500
-
 ferricytochrome c mutant enzyme D113A, using 60 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 4500
-
 ferricytochrome c mutant enzyme E115A/E116A, using 210 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 4700
-
 oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide mutant enzyme D113A, using 210 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 4800
-
 oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide mutant enzyme D113A, using 110 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 4800
-
 oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide mutant enzyme D113A, using 60 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 5000
-
 oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide mutant enzyme D113A, using 10 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 5000
-
 oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide mutant enzyme E115A/E116A, using 210 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 5300
-
 oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide mutant enzyme D113A, using 30 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 5800
-
 oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide mutant enzyme E115A/E116A, using 110 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 6000
-
 ferricytochrome c mutant enzyme E115A/E116A, using 110 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 6500
-
 oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide mutant enzyme E115A/E116A, using 60 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 6800
-
 oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide mutant enzyme E115A/E116A, using 30 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 7000
-
 ferricytochrome c mutant enzyme E115A/E116A, using 60 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 7200
-
 oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide mutant enzyme E115A/E116A, using 10 mM oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 8300
-
 ferricytochrome c wild type enzyme, using 30 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 10000
-
 ferricytochrome c mutant enzyme D113A, using 30 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus
1.6.2.4 10500
-
 ferricytochrome c mutant enzyme E115A/E116A, using 30 mM ferricytochrome c as substrate, in 20 mM HEPES buffer (pH 7.5), at 25°C Rattus norvegicus